Type III secretion antigen delivery system assembled in non-replicating platform

空肠弯曲杆菌是美国食源性腹泻最常见的原因。尽管它是 最常与自限性腹泻病有关，一小部分感染患者会发展为 更严重的神经退行性并发症称为格林-巴利综合征。尽管有空闲时间 在空肠弯曲菌基因组的整个核苷酸序列中，人们对其作用机制知之甚少 致病性。与空肠弯曲菌感染相关的病理学，结合在 实验动物和组织培养系统表明，疾病可能是发展的结果 这些细菌和肠道上皮细胞之间有一个密切的功能接口。作为一个结果 通过这些亲密的相互作用，空肠弯曲菌可以接触到排列在肠道上皮细胞，并可以 刺激促炎细胞因子的产生。这些事件的分子和细胞基础 人们对此了解甚少。这项研究项目的长期目标是更好地了解 空肠弯曲菌与宿主细胞相互作用的分子和细胞生物学。我们建议执行以下内容 具体目标：1)确定调节空肠弯曲菌与宿主细胞相互作用的决定因素；2) 加深对导致空肠弯曲菌摄取和存活的关键事件的细胞生物学的理解 在肠上皮细胞内；3)研究空肠弯曲菌的代谢重新编程，使其 在宿主细胞内存活；以及4)确定空肠弯曲菌在体外或体内生长后的蛋白多糖。 哺乳动物细胞。

Campylobacter jejuni is the most common cause of food-born diarrhea in the United States. Although it is most often associated with self-limiting diarrheal disease, a small proportion of infected patients develop a more serious neuro-degenerative complication known as Guillain-Barre Syndrome. Despite the availability of the entire nucleotide sequence of the C. jejuni genome, remarkably little is known about its mechanisms of pathogenicity. The pathology associated with C. jejuni infections, combined with a variety of observations in experimental animals and tissue culture systems, suggest that disease may be the result of the development of an intimate functional interface between these bacteria and intestinal epithelial cells. As a consequence of these intimate interactions, C. jejuni can gain access to the cells that line the intestinal epithelium and can stimulate the production of pro-inflammatory cytokines. The molecular and cellular bases for these events are poorly understood. The long term goal of this research project is to gain a better understanding of the molecular and cell biology of the interactions of C. jejuni with host cells. We propose to execute the following specific aims: 1) To identify C. jejuni determinants which mediate its interactions with host cells; 2) To deepen the understanding of the cell biology of critical events leading to C. jejuni uptake into and survival within intestinal epithelial cells; 3) To investigate the C. jejuni metabolic reprogramming that allows its survival within host cells; and 4) To define the proteoglycome of C. jejuni after growth in vitro or within mammalian cells.