New Ops - - RNAi Screening Projects

研讨会2.基因组规模的RNAi筛选 一般说明 RNA干扰(RNAi)是一种非常强大的基因功能探测技术。从功能上讲，RNAi 其效用源于小双链RNA(小干扰RNA或siRNAs)对 具体地说，沉默基因包含的序列与小RNA中的序列相同。由于可以使用RNAi 沉默特定的病原体基因或沉默宿主细胞中可能涉及的特定基因 致病过程，这是研究宿主-病原体相互作用的一种非常有价值的方法， 确定单个微生物毒力因子的特征，并识别参与病原菌复制的宿主基因 和致命性。本研讨会将介绍RNAi技术和基因组规模的RNAi筛选能力 可在哈佛医学院的ICCB-L筛查设施获得。

Workshop 2. Genome-scale RNAi Screening General description RNA interference (RNAi) is an extremely powerful technology for probing gene function. Functionally, RNAi derives its utility from the ability of small double-stranded RNAs (small interfering RNA or siRNAs) to specifically silence genes containing sequences identical to those in the small RNAs. Since RNAi can be used to silence either specific pathogen genes or to silence specific genes in the host cell that might be involved in the pathogenic process, it represents an extremely valuable approach for studying host-pathogen interactions, characterizing individual microbial virulence factors, and identifying host genes involved in pathogen replication and virulence. This workshop will describe RNAi technology and genome-scale RNAi screening capabilities available at the ICCB-L screening facility at Harvard Medical School.