New Ops - - RNAi Screening Projects

新行动 - - RNAi 筛选项目

• 批准号: 7942387
• 负责人: CAROLINE E SHAMU
• 金额: $ 16.77万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-25 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7942387
• 关键词: Biological Assay; Cells; Data Analyses; Double-Stranded RNA; Educational workshop; Gene Silencing; Genes; Genome; Goals; Image; Individual; New England; Process; RNA Interference; Reader; Research Personnel; Screening procedure; Small Interfering RNA; Small RNA; Technology; Virulence; Virulence Factors; assay development; base; biodefense; design; gene function; medical schools; microbial; pathogen

Workshop 2. Genome-scale RNAi Screening General description RNA interference (RNAi) is an extremely powerful technology for probing gene function. Functionally, RNAi derives its utility from the ability of small double-stranded RNAs (small interfering RNA or siRNAs) to specifically silence genes containing sequences identical to those in the small RNAs. Since RNAi can be used to silence either specific pathogen genes or to silence specific genes in the host cell that might be involved in the pathogenic process, it represents an extremely valuable approach for studying host-pathogen interactions, characterizing individual microbial virulence factors, and identifying host genes involved in pathogen replication and virulence. This workshop will describe RNAi technology and genome-scale RNAi screening capabilities available at the ICCB-L screening facility at Harvard Medical School.

讲习班2.基因组规模的RNAi筛选 一般描述 RNA干扰（RNAi）是一种非常强大的基因功能检测技术。在功能上，RNAi 它的用途来自小双链RNA（小干扰RNA或siRNA）的能力， 特异性沉默含有与小RNA中的序列相同的序列的基因。由于RNAi可以用于 沉默特定的病原体基因或沉默宿主细胞中可能参与 致病过程，它代表了研究宿主-病原体相互作用的一种非常有价值的方法， 表征单个微生物毒力因子，并鉴定参与病原体复制的宿主基因 和毒性。本次研讨会将介绍RNAi技术和基因组规模的RNAi筛选能力 可在哈佛医学院的ICCB-L筛查设施获得。