FASEB SRC on Dynamic DNA Structures in Biology

FASEB SRC 关于生物学中动态 DNA 结构

• 批准号: 9543618
• 负责人: SUSAN THOMAS LOVETT
• 金额: $ 0.5万
• 依托单位: FEDERATION OF AMER SOC FOR EXPER BIOLOGY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2019-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9543618
• 关键词: Adopted; Aging; Area; B-DNA; Biology; Collaborations; DNA; DNA Sequence; DNA Structure; Development; Discipline; Disease; Educational workshop; Environment; Fostering; Funding; Genetic Diseases; Genetic Processes; Genetic Recombination; Genetic Transcription; Genome; Genomic DNA; Goals; Hand; Health; Hereditary Disease; Human; Immune response; Immunoglobulin Class Switching; Inherited; Investigation; Left; Malignant Neoplasms; Molecular Conformation; Outcome; Participant; Pathologic; Pathologic Processes; Physiological Processes; Process; Property; Repetitive Sequence; Replication-Associated Process; Request for Proposals; Research Personnel; Resolution; Role; Science; Scientist; Structure; Therapeutic; Training; Transact; Transcriptional Activation; Trinucleotide Repeats; Work; career; collaborative environment; developmental disease; genome editing; genome integrity; human disease; meetings; nervous system disorder; novel; posters; preference; programs; summer research; symposium

This proposal requests partial support for the FASEB Summer Research Conference on Dynamic DNA Structures in Biology, which will be held July 15-20, 2018, in Olean, NY. For decades after its discovery, DNA was believed to be a rigid, right-handed double helix. It is now appreciated, however, that DNA is highly dynamic molecule and can assume an enormous variety of conformations such as cruciforms, left-handed helices, three- and four-stranded helices, and slip- stranded configurations. Repetitive DNA sequences, which are overrepresented in genomic DNA, are particularly prone to such structural transitions. The transient denaturation of the double helix that occurs during most DNA transactions (transcription, replication and recombination) also promotes dynamic transitions in DNA structure. The cellular environment can also influence structure formation. Studies have confirmed that structure-prone DNA sequences are central to the normal functioning of the genome. Transitions in DNA structure are important for regulating the activation of transcription and the specialized recombination responsible for immunoglobulin class switching. Unscheduled transitions, however, can have pathological consequences. The expansion of structure-prone repeats underlies more than thirty hereditary neurological and developmental diseases in humans and dynamic DNA structures are associated with genome rearrangements observed in many human cancers. The long-term objective of this Conference is to enhance our understanding of how dynamic DNA structures form, how they contribute to normal genetic processes and how they promote pathological outcomes such as genetic disease, cancer and aging. Our aims are to explore DNA structural transitions, to define novel mechanisms that promote formation and resolution of dynamic DNA structures, to stimulate collaborations, and to foster the long-term development of this important area by promoting participation of junior scientists. To that end, we will convene ~100 participants for five intense and highly interactive days of science. The program will include two opening keynote talks, eight morning/evening scientific sessions and informal afternoon poster sessions. New this year will be a session on genome editing. In addition, another new feature will be to involve early career scientists in the planning and content of “meet the speaker” and career workshop discussions at the conference. One third of the talks will be chosen from submitted abstracts, with preference given to trainees and junior investigators. For many trainees, this will be the first opportunity to give a talk at a scientific meeting. The significance and strength of this Conference is that it uniquely brings together investigators with a common focus on dynamic DNA transitions, but who have highly varied backgrounds, expertise and experimental approaches.

该提案要求部分支持FASE B夏季动态DNA研究会议 生物学中的结构，将于2018年7月15日至20日在纽约州奥利恩举行。 在发现DNA后的几十年里，人们一直认为DNA是一个刚性的右旋双螺旋结构。它是 然而，现在人们意识到，DNA是高度动态的分子，可以呈现出巨大的多样性 构象，如十字形，左手螺旋，三链和四链螺旋，以及滑移- 搁浅的配置。在基因组DNA中过度表达的重复DNA序列是 特别容易发生这样的结构性转变。双螺旋的瞬时变性 发生在大多数DNA交易期间(转录、复制和重组)也促进 DNA结构中的动态转变。细胞环境也会影响结构的形成。 研究证实，易于结构的DNA序列是细胞正常功能的核心 基因组。DNA结构的转变对于调节转录和转录的激活非常重要 负责免疫球蛋白类别转换的专门化重组。计划外的过渡， 然而，可能会产生病理性的后果。易于构造的重复序列的扩展是基础 人类30多种遗传性神经和发育疾病与动态DNA 在许多人类癌症中观察到的结构与基因组重排有关。 本次会议的长期目标是增进我们对DNA如何具有活力的理解 结构的形成，它们如何对正常的遗传过程做出贡献，以及它们如何促进病理 遗传疾病、癌症和衰老等后果。我们的目标是探索DNA结构 转变，以定义促进动态DNA的形成和分解的新机制 结构，以促进合作，并通过以下方式促进这一重要领域的长期发展 促进初级科学家的参与。为此，我们将召集约100名与会者，共5人 紧张和高度互动的科学日。该节目将包括两次开场主旨演讲， 八个上午/晚上的科学会议和非正式的下午海报会议。新的一年将是 关于基因组编辑的会议。此外，另一个新功能将是让早期职业科学家参与进来 在大会的《与演讲者见面》和职业研讨会讨论的策划和内容中。 三分之一的演讲将从提交的摘要中选出，受训人员和 初级调查员。对于许多实习生来说，这将是他们第一次有机会在科学研讨会上演讲。 开会。本会议的意义和力量在于，它以独特的方式将 共同关注动态DNA转变的研究人员，但他们的研究结果差异很大 背景、专业知识和实验方法。