Core B - Advance Imaging Core

核心 B - 高级成像核心

• 批准号: 10397005
• 负责人: Jose Javier Bravo-Cordero
• 金额: $ 29.39万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-15 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10397005
• 关键词: Aging; Alzheimer' s Disease; Animals; Antibodies; Area; Autophagocytosis; Brain; Cell model; Characteristics; Communities; Computer software; Confocal Microscopy; Core Facility; Cost efficiency; Custom; Data; Data Analyses; Databases; Development; Disease; Dyes; Electron Microscopy; Equipment; Experimental Designs; Fluorescence; Funding; Geroscience; Goals; Image; Image Analysis; Imaging Techniques; Imaging technology; Immunofluorescence Immunologic; In Vitro; Individual; Institution; Light; Liver; Methods; Microscopy; Molecular; Monitor; Mus; Operative Surgical Procedures; Organ; Organelles; Pancreas; Peripheral; Physiological; Preparation; Procedures; Protocols documentation; Quality Control; Reagent; Reporter; Research Personnel; Resolution; Resources; Sampling; Schools; Services; Specimen; Standardization; Techniques; Technology; Testing; Text; Therapeutic; Time; Tissues; Training; Translations; base; cost efficient; data integration; future implementation; high resolution imaging; imaging capabilities; imaging modality; imaging platform; in vivo; in vivo imaging; innovation; instrumentation; interest; intravital imaging; intravital microscopy; live cell imaging; lymph nodes; member; molecular dynamics; mouse model; nanoscale; new technology; programs; proteostasis; proteotoxicity; routine imaging; single molecule; spatiotemporal; tool; two photon microscopy; two-photon

Summary The Proteostasis Advanced Imaging Core (Core B) referred in the Program Project (PP) text as “Image Core” will serve the four projects and the Innovation component of Core D of this PP. The long-term goal is to provide support on image-based studies to understand the cellular and subcellular changes in autophagy dynamics during aging and in the context of Alzheimer disease (AD)-related proteotoxicity. The specific aims of the core are: 1) to provide an imaging resource customized for the study of proteostasis in aging that will assist the PP investigators in the experimental preparation related to: basic microscopy techniques (wide-field microscopy, confocal microscopy, live cell imaging - at Mount Sinai/Einstein) and electron microscopy (at Einstein); 2) to provide a state-of-the art in vivo imaging platform based on two-photon microscopy (Innovation Unit - Mount Sinai) for dynamic analysis of changes in autophagy in brain and peripheral tissues in physiological aging and in disease; 3) to implement clearing imaging techniques and light-sheet microscopy for volume imaging to study autophagy in whole organs (Innovation Unit); 4) to develop STED superresolution imaging methods to study the dynamics of autophagy at the organelle level by nanoscale imaging (Innovation Unit). Components: 1) The Imaging unit provides advice on sample preparation/processing, techniques and procedures for imaging in vivo and fixed samples. Performs electron microscopy (at Einstein) and specialized imaging procedures (at Mount Sinai) such as intravital microscopy, light-sheet imaging, STED superresolution and assists with imaging data interpretation; 2) The innovation unit (at Mount Sinai) will develop and implement procedures specific for analysis of autophagy at the tissue level (light-sheet imaging, intravital microscopy) and at the single molecule level (superresolution imaging) in aging and AD. Services: The core will continue offering i) assistance with regular wide-field microscopy, real-time live cell imaging, confocal and electron microscopy, ii) distributing common reagents (antibodies, probes and fluorescence dyes), iii) sharing detailed protocols for image-based procedures and iv) compiling the image- based information for the PP data base. In addition, during this period the core has now incorporated: i) high- resolution imaging procedures such as intravital two-photon microscopy, superresolution STED imaging and light-sheet microscopy for volume imaging, ii) assistance with sample preparation for these procedures (in vivo mouse preparation, clearing methods) and iii) access to new image analysis software (IMARIS, ARIVIS, Huygens) available at the Microscopy CoRE at Mount Sinai. Relevance: The new technology incorporated in the core now allows dynamic and molecular resolution imaging to study aging and AD-related changes in autophagy at the subcellular level. This information is essential for future implementation of therapeutic approaches aiming at reverting these changes. The centralization of these new procedures optimizes cost efficiency and guarantees standardization and integration of the different groups.

总结 Proteostasis高级成像核心（核心B）在程序项目（PP）文本中称为“图像 核心”将服务于这四个项目和本PP核心D的创新部分。 长期目标是提供基于图像的研究支持，以了解细胞和亚细胞 自噬动力学在衰老过程中的变化和阿尔茨海默病（AD）相关的蛋白毒性的背景下。 该核心的具体目标是：1）为蛋白质稳态研究提供定制的成像资源 在老化，这将有助于PP研究人员在实验准备有关：基础显微镜 技术（宽视野显微镜，共聚焦显微镜，活细胞成像-在西奈山/爱因斯坦）和电子 显微镜（在爱因斯坦）; 2）提供基于双光子显微镜的最先进的体内成像平台 （创新单位-西奈山），用于动态分析脑和外周组织中自噬的变化， 生理老化和疾病; 3）实施透明成像技术和光片显微镜， 研究整个器官自噬的体积成像（创新单元）; 4）开发STED超分辨率 通过纳米成像在细胞器水平研究自噬动力学的成像方法（创新 单位）。 组件：1）成像单元提供有关样品制备/处理、技术和 用于体内和固定样品成像的程序。执行电子显微镜（在爱因斯坦）和专业 成像程序（在西奈山），如活体显微镜，光片成像，STED超分辨率 并协助成像数据解释; 2）创新单位（在西奈山）将开发和实施 在组织水平上分析自噬的特定程序（光片成像，活体显微镜）， 在单分子水平（超分辨率成像）在老化和AD。 服务：核心将继续提供i）定期宽视野显微镜，实时活细胞 成像、共聚焦和电子显微镜，ii）分配常用试剂（抗体、探针和 荧光染料），iii）共享用于基于图像的程序的详细协议，以及iv）编译图像- 基于PP数据库的信息。此外，在此期间，核心方案现已纳入： 分辨率成像程序，如活体双光子显微镜，超分辨率STED成像和 用于体积成像的光片显微镜，ii）协助这些程序的样品制备（体内 小鼠制备，清除方法）和iii）使用新的图像分析软件（IMARIS，ARIVIS， Huygens），可在西奈山的显微镜核心实验室获得。 相关性：核心中包含的新技术现在允许动态和分子分辨率成像 在亚细胞水平上研究衰老和AD相关的自噬变化。这些信息对于以下方面至关重要： 未来实施旨在逆转这些变化的治疗方法。这些集中化的 新程序优化了成本效益，并保证了不同群体的标准化和一体化。