Core B - Advance Imaging Core

核心 B - 高级成像核心

• 批准号: 10602538
• 负责人: Jose Javier Bravo-Cordero
• 金额: $ 29.39万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-15 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10602538
• 关键词: Aging; Alzheimer' s Disease; Animals; Antibodies; Area; Autophagocytosis; Brain; Cell model; Characteristics; Communities; Computer software; Confocal Microscopy; Core Facility; Cost efficiency; Data; Data Analyses; Databases; Dedications; Development; Disease; Dyes; Electron Microscopy; Equipment; Experimental Designs; Fluorescence; Funding; Geroscience; Goals; Image; Image Analysis; Imaging Techniques; Imaging technology; Immunofluorescence Immunologic; In Vitro; Individual; Institution; Light; Liver; Methods; Microscopy; Molecular; Monitor; Mus; Operative Surgical Procedures; Organ; Organelles; Pancreas; Peripheral; Physiological; Preparation; Procedures; Protocols documentation; Quality Control; Reagent; Reporter; Research Personnel; Resolution; Resources; Sampling; Schools; Services; Specimen; Standardization; Techniques; Technology; Testing; Text; Therapeutic; Time; Tissues; Training; Translations; cost; data integration; future implementation; high resolution imaging; imaging capabilities; imaging modality; imaging platform; in vivo; in vivo imaging; innovation; instrumentation; interest; intravital imaging; intravital microscopy; live cell imaging; lymph nodes; member; molecular dynamics; mouse model; nanoscale; new technology; programs; proteostasis; proteotoxicity; routine imaging; single molecule; spatiotemporal; superresolution imaging; superresolution microscopy; tool; two photon microscopy; two-photon; ultra high resolution

Summary The Proteostasis Advanced Imaging Core (Core B) referred in the Program Project (PP) text as “Image Core” will serve the four projects and the Innovation component of Core D of this PP. The long-term goal is to provide support on image-based studies to understand the cellular and subcellular changes in autophagy dynamics during aging and in the context of Alzheimer disease (AD)-related proteotoxicity. The specific aims of the core are: 1) to provide an imaging resource customized for the study of proteostasis in aging that will assist the PP investigators in the experimental preparation related to: basic microscopy techniques (wide-field microscopy, confocal microscopy, live cell imaging - at Mount Sinai/Einstein) and electron microscopy (at Einstein); 2) to provide a state-of-the art in vivo imaging platform based on two-photon microscopy (Innovation Unit - Mount Sinai) for dynamic analysis of changes in autophagy in brain and peripheral tissues in physiological aging and in disease; 3) to implement clearing imaging techniques and light-sheet microscopy for volume imaging to study autophagy in whole organs (Innovation Unit); 4) to develop STED superresolution imaging methods to study the dynamics of autophagy at the organelle level by nanoscale imaging (Innovation Unit). Components: 1) The Imaging unit provides advice on sample preparation/processing, techniques and procedures for imaging in vivo and fixed samples. Performs electron microscopy (at Einstein) and specialized imaging procedures (at Mount Sinai) such as intravital microscopy, light-sheet imaging, STED superresolution and assists with imaging data interpretation; 2) The innovation unit (at Mount Sinai) will develop and implement procedures specific for analysis of autophagy at the tissue level (light-sheet imaging, intravital microscopy) and at the single molecule level (superresolution imaging) in aging and AD. Services: The core will continue offering i) assistance with regular wide-field microscopy, real-time live cell imaging, confocal and electron microscopy, ii) distributing common reagents (antibodies, probes and fluorescence dyes), iii) sharing detailed protocols for image-based procedures and iv) compiling the image- based information for the PP data base. In addition, during this period the core has now incorporated: i) high- resolution imaging procedures such as intravital two-photon microscopy, superresolution STED imaging and light-sheet microscopy for volume imaging, ii) assistance with sample preparation for these procedures (in vivo mouse preparation, clearing methods) and iii) access to new image analysis software (IMARIS, ARIVIS, Huygens) available at the Microscopy CoRE at Mount Sinai. Relevance: The new technology incorporated in the core now allows dynamic and molecular resolution imaging to study aging and AD-related changes in autophagy at the subcellular level. This information is essential for future implementation of therapeutic approaches aiming at reverting these changes. The centralization of these new procedures optimizes cost efficiency and guarantees standardization and integration of the different groups.

摘要 蛋白质平衡高级成像核心(核心B)在计划项目(PP)文本中被称为“图像 CORE“将为四个项目和本方案的核心D创新部分提供服务。 长期目标是为基于图像的研究提供支持，以了解细胞和亚细胞 在衰老和阿尔茨海默病(AD)相关蛋白毒性背景下自噬动力学的变化。 核心的具体目标是：1)为蛋白质代谢研究提供定制的成像资源 在老化方面，这将帮助PP调查人员进行与以下相关的实验准备：基础显微镜 技术(广域显微镜、共聚焦显微镜、活细胞成像--在西奈山/爱因斯坦)和电子 显微镜(在爱因斯坦)；2)提供基于双光子显微镜的最先进的活体成像平台 (创新单位-西奈山)，用于动态分析脑组织和周围组织中自噬的变化 生理性衰老和疾病；3)实施透明成像技术和光片显微镜 体积成像用于研究整个器官的自噬(创新单位)；4)发展STED超分辨率 通过纳米级成像研究细胞器水平自噬动力学的成像方法(创新 单位)。 组成部分：1)成像股提供关于样品准备/处理、技术和 活体和固定样品的成像程序。执行电子显微镜(在爱因斯坦)和专门的 成像程序(在西奈山)，如活体显微镜、薄片成像、STED超分辨率 并协助成像数据解释；2)创新股(在西奈山)将开发和实施 在组织水平上分析自噬的特定程序(光片成像、活体显微镜)和 在单分子水平上(超分辨率成像)，在衰老和AD。 服务：核心将继续提供：1)利用常规广域显微镜、实时活细胞技术提供协助 成像、共聚焦和电子显微镜，二)分发通用试剂(抗体、探针和 荧光染料)，iii)分享基于图像的程序的详细协议，以及iv)编辑图像- 基于PP数据库的信息。此外，在此期间，核心现已纳入：i)高- 分辨率成像程序，如活体双光子显微镜，超分辨率STED成像和 用于体积成像的光片显微镜，二)协助这些程序的样品制备(活体内 鼠标准备、清除方法)和三)使用新的图像分析软件(IMARIS、ARIVIS、 惠更斯)可在西奈山的显微镜核心获得。 相关性：集成在核心中的新技术现在可以进行动态和分子分辨率成像 在亚细胞水平上研究衰老和AD相关自噬的变化。此信息对以下各项至关重要 未来实施旨在逆转这些变化的治疗方法。这些资源的集中化 新的程序优化了成本效率，并保证了不同群体的标准化和一体化。