Selective Control of Synaptically-Connected Circuit Elements by Interluminescence - Diversity Supplement SILVAGNOLI

通过间发光选择性控制突触连接的电路元件 - Diversity Supplement SILVAGNOLI

• 批准号: 10731169
• 负责人: UTE H HOCHGESCHWENDER
• 金额: $ 7.98万
• 依托单位: CENTRAL MICHIGAN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10731169
• 关键词: Acute; Address; Award; BRAIN initiative; Behavior; Brain; Complex; Data; Development; Disease; Education; Electrophysiology (science); Elements; Ensure; Epilepsy; Experimental Models; Fluorescence Microscopy; Fostering; Foundations; Goals; Injections; Memory Loss; Mental Depression; Mental disorders; Mentors; Methods; Mission; Neurosciences; Parents; Public Health; Research; Role; Schizophrenia; Solid; Synaptic plasticity; Therapeutic; Time; Training; United States National Institutes of Health; Validation; Work; addiction; autism spectrum disorder; career; career development; design; experience; experimental study; extracellular; in vivo; innovation; interest; meetings; nervous system disorder; neuroregulation; new technology; novel; parent grant; tool

PROJECT SUMMARY/ABSTRACT The Diversity Supplement candidate will be trained in all aspects of validating Interluminescence in vivo, from stereotaxic injections of AAV, verifying construct expression through fluorescence microscopy, to acute extracellular recordings and computational sophisticated electrophysiological analysis. The experimental approach will allow the candidate to assess Interluminescence within a well-defined experimental model while building a solid conceptual and experimental foundation for his graduate work. The training experiences the candidate will gain are critical for his understanding, designing, and interpretation of state-of-the-art neuroscience experiments. The training plan takes into consideration that the candidate is at the beginning of his graduate training, gives him a ‘hot start’ in his education, and ensures that the candidate will have an exceedingly strong background for meeting his career goals. His training will have ranged from hands- on experience in designing and executing in vivo experiments for controlling neural activity and behavior to evaluating and interpreting the collected data. Therefore, this training in in vivo electrophysiology will not only significantly contribute to the parent grant but will specifically provide him the experience required to pursue his long-term interest in synaptic plasticity and behavior. The proposed training outlined in the application is ideal given the candidate’s early career stage and is tailored to having the greatest impact at the earliest possible time point. An established team training strategy between the two mentors will foster the candidate’s ability to swiftly reach a level where he can independently investigate the role of synaptically-connected circuits. The proposed supplement activities will increase the parent award’s overall impact within the original scope of award while at the same time advancing the training and career development of the candidate.

项目摘要/摘要 多样性补充候选人将接受在体内验证相互发光的所有方面的培训，从 立体定向注射AAV，通过荧光显微镜验证构建体表达，对急性 细胞外记录和计算复杂的电生理分析。实验性的 方法将允许候选人在明确定义的实验模型中评估相互发光，而 为他的研究生工作奠定了坚实的概念和实验基础。 应聘者将获得的培训经验对他的理解、设计和解释至关重要 最先进的神经科学实验。培训计划考虑到应聘者在 他的研究生培训的开始，给了他一个热的开始他的教育，并确保候选人将 有一个非常强大的背景，以实现他的职业目标。他的训练范围从手到手- 关于设计和实施控制神经活动和行为的活体实验的经验 对收集到的数据进行评估和解释。因此，这种体内电生理学的训练不仅会 为家长拨款作出重大贡献，但会特别为他提供所需的经验，使他 对突触可塑性和行为的长期兴趣。 考虑到应聘者职业生涯的早期阶段，申请表中提出的培训是理想的，而且是量身定做的 在尽可能早的时间点产生最大的影响。一项既定的团队训练战略 这两位导师将培养候选人迅速达到他能够独立调查的水平的能力 突触连接回路的作用。 拟议的补充活动将在原范围内增加父奖项的整体影响 奖励，同时推进候选人的培训和职业发展。