Selective Control of Synaptically-Connected Circuit Elements by Interluminescence - Diversity Supplement: E. CRESPO

通过间发光选择性控制突触连接的电路元件 - 多样性补充：E. CRESPO

• 批准号: 10406018
• 负责人: UTE H HOCHGESCHWENDER
• 金额: $ 11.32万
• 依托单位: CENTRAL MICHIGAN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-15 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10406018
• 关键词: Acute; Address; Award; BRAIN initiative; Behavior; Brain; Complement; Complex; Data; Development; Disease; Electrophysiology (science); Elements; Epilepsy; Experimental Models; Fostering; Goals; Memory Loss; Mental Depression; Mental disorders; Mentors; Methods; Mission; Molecular; Neurosciences; Parents; Protein Engineering; Public Health; Research; Role; Schizophrenia; Therapeutic; Time; Training; United States National Institutes of Health; Validation; Work; addiction; autism spectrum disorder; career; career development; design; experience; extracellular; in vivo; innovation; meetings; neocortical; nervous system disorder; new technology; novel; optogenetics; parent grant; relating to nervous system; tool; tool development

PROJECT SUMMARY/ABSTRACT The Diversity Supplement candidate will be trained in all aspects of validating Interluminescence in vivo with acute extracellular recordings and computational sophisticated electrophysiological analysis. The experimental approach will allow the candidate to assess Interluminescence with a well-defined experimental model to dissect cortical dynamics. The training experience the candidate will gain are critical for his understanding, designing, and interpretation of state-of-the-art neural data. The training plan will complement his prior extensive training in molecular and cellular neuroscience. Following this experience in applying chemogenetic and optogenetic tools in vivo, the candidate will have an exceedingly strong background for meeting his career goals. His training will have ranged from hands-on experience developing novel probes at the level of the engineered protein, to their intended use in controlling neural activity and behavior. Therefore, this training in in vivo electrophysiology will not only significantly contribute to the parent grant but will specifically provide him the experience required to dissect neocortical dynamics and come “full circle” in the tool development pipeline. The proposed training outlined in the application is ideal given the candidate’s productive track record. An established team training strategy between the two mentors will foster the candidate’s ability to independently investigate the role of synaptically-connected circuits. The proposed supplement activities will increase the parent award’s overall impact within the original scope of award while at the same time advancing the training and career development of the candidate.

项目总结/摘要 多样性补充候选人将接受体内内发光验证的所有方面的培训， 急性细胞外记录和计算复杂的电生理分析。实验 该方法将允许候选人使用定义明确的实验模型来评估Interluminescence， 皮质动力学 候选人将获得的培训经验对他理解，设计和解释 最先进的神经数据培训计划将补充他之前在分子和生物学方面的广泛培训。 细胞神经科学根据在体内应用化学遗传学和光遗传学工具的经验， 候选人将有一个非常强大的背景，以满足他的职业目标。他的训练 从在工程蛋白水平上开发新型探针的实践经验，到它们的预期用途， 控制神经活动和行为。因此，这种体内电生理学的训练不仅将 显着有助于家长补助金，但将专门为他提供所需的经验，解剖 新皮层动力学，并在工具开发管道中“完整循环”。 考虑到候选人的生产记录，申请中列出的拟议培训是理想的。一个 两个导师之间建立的团队培训策略将培养候选人独立 研究突触连接回路的作用。 拟议的补充活动将在原有的范围内增加家长奖的总体影响， 奖励，同时促进候选人的培训和职业发展。