CRR LIGAND ASSAY AND ANALYSIS CORE

CRR 配体测定和分析核心

• 批准号: 10017063
• 负责人: John C Marshall
• 金额: $ 44.4万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-13 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10017063
• 关键词: Award; Biological Assay; Clinical; Consultations; Core Facility; Data; Data Analyses; Ensure; Fertility; Individual; Infertility; Ligands; Location; Measurement; Measures; Methodology; Methods; National Institute of Child Health and Human Development; Nature; Performance; Polycystic Ovary Syndrome; Publications; Reproducibility; Research; Research Personnel; Services; Standardization; Testing; Training; Visually Impaired Persons; cost effective; data reduction; instrumentation; programs; quality assurance; reproductive; reproductive hormone

The accurate and reliable measurement of reproductive hormones and related analytes are essential for successful research throughout the Fertility and Infertility Branch (FIB) of the NICHD and beyond. The Ligand Assay and Analysis Core of the Clinical and Basic Studies in Polycystic Ovary Syndrome group has been providing this service for over two decades. This open-access core is critical for our and other NCTRI centers, as well as an array of individual and program-type awards made through FIB, broader NICHD and other approved users. The Core dedicates considerable effort to characterizing each assay and to continually reviewing important parameters for each test to maintain rigor, reproducibility and optimal assay performance. The Core has continually evolved, both implementing well-characterized, state-of-the-art assay methods and developing new in-house methodologies to meet the needs of investigators. The Core provides a centralized location with a highly-trained technical staff and the appropriate instrumentation to maintain exceptional standardized methods. This reduces unnecessary and expensive redundancy of effort by other investigators and provides the further advantage that individuals blinded as to the experimental details perform the analysis. The Core's emphasis on maintaining appropriate quality assurance measures ensures analyte data avoids assay reliability pitfalls that may compromise validity and the scientific interpretation of the findings. In addition to providing high-quality, cost-effective assay and analysis services, and developing new methods, the core provides assistance and consultation on assay methodology, data reduction/analysis and data interpretation to investigators that access our Ligand Core Facility. The utility and essential nature of this core to reproductive research is demonstrated by its contribution to 119 primary publications and four reviews since 2014.

准确、可靠地测量生殖激素和相关分析物对于 在整个NICHD的生育和不孕处(FIB)和其他地方成功地进行了研究。The Ligand 多囊卵巢综合征临床及基础研究的检测与分析核心 提供这项服务已有二十多年。这一开放接入的核心对我们和其他NCTRI中心至关重要， 以及通过FIB、更广泛的NICHD和其他机构颁发的一系列个人和计划类型的奖项 已批准的用户。核心投入了相当大的努力来描述每一种化验方法，并不断地 审查每个测试的重要参数，以保持严密性、重复性和最佳的测试性能。 核心不断发展，既实现了特征良好的、最先进的分析方法，也 开发新的内部方法，以满足调查人员的需要。核心提供了一个集中的 拥有训练有素的技术人员和适当的仪器设备，以保持卓越的地理位置 标准化方法。这减少了其他调查人员不必要且代价高昂的重复工作 并提供了对实验细节视而不见的个体执行分析的进一步优势。 核心强调保持适当的质量保证措施，确保分析物数据避免 可能会影响有效性和对结果的科学解释的分析可靠性陷阱。此外 提供高质量、高性价比的化验和分析服务，以及开发新的方法，核心 在分析方法、数据减少/分析和数据解释方面提供帮助和咨询 进入我们的配基核心设施的调查人员。这个核心对生殖的效用和本质 自2014年以来，其对119份主要出版物和4份评论的贡献证明了这项研究。