REPOSITORY PULL FOR SUICIDE AND MATERNAL IMMUNE ACTIVITY STUDY

该项目需要访问来自围产期合作项目的2371份样本，这些样本已储存在-20 ℃的储存库中。 考虑到非最佳储存条件和样本的极端老化，我们的经验是，许多样本被干燥或蒸发到无法获得所需的3 ml血清的程度。 我们使用这些样品的最后一个项目，我们发现4%的原始样品要求不可用。 存储库指出，在某些情况下，这可能高达40%。 因此，我们正在为储存库提供资源，以额外提取25%的标本，以支付为满足这一要求而重复提取的成本。 这项研究将把1959 - 1966年美国围产期合作项目（CPP）参与者所生的52，966名儿童的数据与国家死亡指数联系起来，确定他们到2013年的生命状态，并调查自杀完成的产前，社会经济，行为，认知和神经风险。 我们将进行一项巢式病例对照研究，以调查妊娠期免疫活性对胎儿自杀起源的贡献。 DIPHR已经在NICHD-DIPHR储存库中从CPP参与者中确定了2371份血清标本，我们将比较自杀死亡的后代和匹配的非自杀对照组之间的免疫系统生物标志物水平。 我们假设生物标志物浓度与母体免疫系统的扰动一致。

This project requires accessing 2371 samples from the Collaborative Perinatal Project that have been stored at the repository at -20C. Given the non-optimal storage conditions and extreme age of the specimens it has been our experience that many of these samples are desiccated or evaporated to the degree that the required 3 ml serum is not attainable. Our last project using these samples we found 4% of the original samples requested were unavailable. The repository has noted that in some case this could be up to 40%. Thus, we are providing resources for the repository to pull an additional 25% specimens to cover the costs of repeated pulls to satisfy this request. This study will link data from 52,966 children born to participants in the United States Collaborative Perinatal Project (CPP) between 1959 – 1966 to the National Death Index, determine their vital status through 2013, and investigate the prenatal, socioeconomic, behavioral, cognitive, and neurologic risks for completed suicide. We will conduct a nested case-control study to investigate the contributions of gestational immune activity to fetal origins of suicide. The DIPHR has identified 2371 serum specimens in the NICHD-DIPHR repository from CPP participants from which we will compare levels of immune system biomarkers between offspring who died by suicide and a matched group of non-suicide controls. We hypothesize biomarker concentrations consistent with a perturbation of the maternal immune system.