STORAGE OF NUMOM2B FETAL DNA ALIQUOTS FROM CORD BLOOD FOR THE PREGNANCY AND PERINATOLOGY BRANCH OF NICHD

NuMoM 2b研究是一项前瞻性观察性研究，纳入了超过10，000例未经产的患者 妇女们从怀孕的头三个月一直跟踪到分娩，以了解这种机制， 不良妊娠结局的病因开始于妊娠早期。 它是由 八个临床试验点。 所有研究参与者都接受了一系列评估，包括 人口统计学、营养、家族史、母体人体测量、医疗 病史、心理社会因素评估、胎儿生长超声测量、胎盘 功能（子宫动脉多普勒）和生物样本收集（母体血清，血浆， 尿液、宫颈/阴道样本、分娩时的胎盘）。 博士印第安纳州大学的哈斯将从每个参与者的脐带血样本中纯化DNA 在NuMom 2b研究中。在他的费用，他将收到货物，并产生DNA从 大约5，526份脐带血样本。 然后，他将返回NICHD三等份 将200微升浓度为50 ng/uL的DNA从每个大约 5,526个样本。 这将导致总共16，578个DNA样本将通过NICHD DASH等项目提供给批准的研究人员。

The NuMoM2b study was a prospective observational study of over 10,000 nulliparous women followed from the first trimester through delivery to understand the mechanism and etiology of adverse pregnancy outcomes starting in the first trimester. It was conducted at eight clinical sites. All study participants underwent serial evaluations including demographics, nutrition, family history, maternal anthropometric measurements, medical history, assessments of psychosocial factors, ultrasound measures of fetal growth, placental function (uterine artery Doppler) and biospecimen collection (maternal serum, plasma, urine, cervical/vaginal samples, placenta at delivery). Dr. Haas of Indiana University will purify DNA from the cord blood specimens from each participant in the NuMom2b study. At his expense he will receive shipment of and generate DNA from approximately 5,526 cord blood specimens. He will then return to NICHD three aliquots of 200 microliters of DNA at a concentration of 50ng/uL from each of the approximately 5,526 specimens. This will result in a total of 16,578 DNA specimens that will be available to approved researchers through programs such as NICHD DASH.