Defining nanomaterial-biological interactions to enhance biocompatibility and bio

定义纳米材料-生物相互作用以增强生物相容性和生物

• 批准号: 7515119
• 负责人: Robyn L Tanguay
• 金额: $ 29.61万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7515119
• 关键词: Academia; Address; Animals; Anti-Inflammatory Agents; Anti-inflammatory; Area; Benign; Biocompatible; Biological; Biological Assay; Biological Markers; Biological Models; Biomedical Research; Biomedical Technology; Bionics; Biosensor; Characteristics; Charge; Chemicals; Classification; Complex; Couples; Cream; Cultured Cells; Data; Detection; Development; Diagnostic; Dose; Drug Delivery Systems; Electronics; Embryo; Engineering; Environment; Environmental Health; Evaluation; Exhibits; Exposure to; Fishes; Gene Expression; Goals; Gold; Histopathology; Home environment; Image; Individual; Industry; Information Dissemination; Investigation; Knowledge; Ligands; Link; Methods; Modeling; Modification; Molecular; Molecular Profiling; Nanotechnology; Oregon; Particle Size; Performance; Personal Satisfaction; Pharmacology; Pregnancy Tests; Process; Property; Prosthesis; Public Health; Range; Reading; Regenerative Medicine; Relative (related person); Research; Route; Safety; Science; Series; Services; Shapes; Site; Skin; Solutions; Structure-Activity Relationship; Surface; System; Testing; Therapeutic; Toxic effect; Transgenic Organisms; Translating; Trust; Universities; Zebrafish; aqueous; base; biomaterial compatibility; chemical property; data mining; design; desire; improved; in vitro Assay; in vivo; knowledge base; nanobiotechnology; nanomaterials; nanoparticle; nanoscale; nanosensors; novel; particle; pathogen; photonics; repository; response; size; tool; vector; zeta potential

DESCRIPTION (provided by applicant): Nanotechnology is an enabling platform that will provide a broad range of novel applications and improved technologies for biomedical science due to the unique physical and chemical properties inherent to nanomaterials. Pertinent to the development of promising biomedical nanotechnologies, and to the safety of nanomaterials in general, is a thorough understanding of nanomaterial-biological interactions. Yet, the principal characteristics that may be predictive of nanomaterial interactions with biological systems have not been elucidated because of the current lack of data, the enormous diversity of nanomaterials, and the lack of coordinated efforts to share findings and translate data into knowledge. The embryonic zebrafish model is a dynamic in vivo system that offers the power of whole-animal investigations with the convenience of cell culture to rapidly evaluate interactions between engineered nanomaterials and biological systems. Investigations using this model system can reveal subtle interactions at multiple levels of biological organization, i.e. molecular, cellular, systems, organismal. Our approach couples the many advantages of the embryonic zebrafish assay with an ideal nanoparticle platform in order to systematically assess the relative influence of various physiochemical parameters on overall biological responses to nanomaterial exposure. High-purity, ligand-functionalized gold nanoparticles (AuNPs) synthesized in aqueous environments can be precisely engineered such that individual aspects of the material can be evaluated independently. It is well understood that data from this emerging field will be extremely diverse including a multitude of widely varying nanomaterials that are being/or will be tested in a broad array of animal systems and in vitro assays. Knowledge of nanomaterial-biological interactions will likely only be arrived at upon inclusion and consideration of the entire body of data produced from global efforts in this research area. To address these needs in the nascent field of nanobiotechnology, our group has developed a collaborative knowledgebase of Nanomaterial-Biological Interactions (NB). The NBI knowledgebase serves as a repository for annotated data on nanomaterial characterization, synthesis methods, and nanomaterial-biological interactions define at multiple levels of biological organization. Relevant computational, analytic and data mining tools will be incorporated into NBI to the framework for species, route, dose and scenario extrapolations and for identification of key data required to predict the biological interactions of nanomaterials. PUBLIC HEALTH RELEVANCE: New nanomaterials are rapidly being developed for a wide range of biomedical applications (e.g. high-performance diagnostic probes, site-selective therapeutics, prosthetics, regenerative medicine, imaging, etc.), so it is surprising that so little is known about how or why nanomaterials interact with biological systems and even less is known about how to design them to exhibit a desired effect in whole animals. The immediate need to gain comprehensive information on biological-nanomaterial interactions requires systematic, collaborative scientific investigation to define nanomaterial-biological interactions and describe how specific properties of nanomaterials govern biological responses. Timely evaluation and dissemination of information on nanomaterial-biological interactions will provide much needed data, improve public trust of the nanotechnology industry, and provide nanomaterial designers in academia and industry with information to direct the development of high-performance, safe nanomaterials and resulting biomedical technologies.

描述（由申请人提供）：纳米技术是一个有利的平台，由于纳米材料固有的独特物理和化学特性，它将为生物医学科学提供广泛的新颖应用和改进的生物医学科学技术。 与有前途的生物医学纳米技术以及一般纳米材料的安全性有关的发展是对纳米材料生物学相互作用的彻底理解。 然而，由于目前缺乏数据，纳米材料的巨大多样性以及缺乏协调的努力来共享发现并将数据转化为知识，因此尚未阐明与生物系统纳米材料相互作用的主要特征。 胚胎斑马鱼模型是一个动态的体内系统，可提供整体研究的力量，并提供细胞培养的便利性，以快速评估工程纳米材料与生物系统之间的相互作用。 使用此模型系统的研究可以揭示生物组织多个级别的微妙相互作用，即分子，细胞，系统，生物体。 我们的方法将胚胎斑马鱼测定的许多优势与理想的纳米颗粒平台相结合，以系统地评估各种生理化学参数对纳米材料暴露的整体生物学反应的相对影响。 可以精确地设计在水性环境中合成的高纯度，配体功能化的金纳米颗粒（AUNP），以便可以独立评估材料的各个方面。 众所周知，来自这个新兴领域的数据将非常多样化，包括在广泛的动物系统和体外测定中进行的许多纳米材料的广泛变化。 纳米材料生物相互作用的知识可能只有在包含并考虑到该研究领域全球努力产生的整个数据时才能得出。 为了满足纳米生物技术的新生领域的这些需求，我们的小组开发了纳米材料生物学相互作用（NB）的协作知识基础。 NBI知识基础是纳米材料表征，合成方法和纳米材料生物学相互作用的注释数据的存储库，在生物组织的多个级别上定义了。 相关的计算，分析和数据挖掘工具将被纳入NBI中，以纳入物种，路线，剂量和场景外推的框架，以及鉴定预测纳米材料生物学相互作用所需的关键数据。 公共卫生相关性：针对广泛的生物医学应用正在迅速开发新的纳米材料（例如，高性能诊断探针，现场选择性治疗剂，假体，修复学，再生医学，成像等），因此，与nananomials的相互作用相互作用以及如何表现出了如何涉及的方式，令人惊讶的是，很少知道如此众所周知，这是如此之多。 获得有关生物纳米材料相互作用的全面信息的直接需求需要系统的，协作的科学研究来定义纳米材料生物学相互作用，并描述纳米材料的特定特性如何控制生物学反应。 及时评估和传播有关纳米材料生物学相互作用的信息将提供急需的数据，改善纳米技术行业的公共信任，并为学术界和行业的纳米材料设计师提供信息，以指导高性能，安全纳米材料的发展，并导致生物医​​学技术。