Cellular Imaging and Morphometric Analysis Core

细胞成像和形态测量分析核心

• 批准号: 10011811
• 负责人: JAMES Francis MCGINNIS
• 金额: $ 21.47万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10011811
• 关键词: 3-Dimensional; Center Core Grants; Collaborations; Computer software; Core Facility; Disease; Equipment; Eye; Fixatives; Fluorescence; Fluorescence Microscopy; Fostering; Funding; Guidelines; Health; Histologic; Hour; Image; Label; Laboratories; Left; Methods; Microscope; Microtome - medical device; Paraffin; Postdoctoral Fellow; Productivity; Recommendation; Research; Research Personnel; Resolution; Scanning; Sepharose; Services; Slide; Stains; Techniques; Tissues; Training; Vision; Vision research; cellular imaging; cryostat; digital imaging; imaging modality; innovation; member; programs; ranpirnase; student training

ABSTRACT Cellular Imaging and Morphometric Analysis Core Vision research at OUHSC has a very strong focus on understanding the function of ocular tissues at the cellular level in health and disease. Labeling, imaging, and quantitative analysis at the tissue, cellular, and subcellular levels are critical to every vision research lab on our campus. Furthermore, the rapid advancement of the field requires that our researchers incorporate new imaging modalities, analytical approaches, and hardware into their research programs. The methods, training, and facilities required for high quality cellular imaging are expensive, necessitating shared core facilities to foster innovative new research approaches and collaborations, to sustain high productivity, and remain at the forefront of the field. The Cellular Imaging and Morphometric Analysis Core provides highly knowledgeable expert support along with the bright field, epifluorescence, and scanning fluorescence microscopy at the macro (MVX10) and micro levelS (FV500 & FV1200). The highly sophisticated software packages (Metamorph and Imaris) enable vision researchers to generate new images and analyze them by software previously unavailable. The training of students, postdoctoral fellows, staff, and PIs in the use of the Cellular Imaging Core facilities greatly expands their investigative capabilities, develops creative ideas, and promotes collaborative interactions.

摘要 细胞成像和形态分析核心 OUHSC的视觉研究非常注重了解眼睛组织的功能， 在健康和疾病的细胞水平。在组织、细胞和组织中进行标记、成像和定量分析。 亚细胞水平对我们校园里的每个视觉研究实验室都至关重要。此外，快速发展 该领域的发展要求我们的研究人员采用新的成像方式，分析方法， 硬件到他们的研究项目中。高质量蜂窝通信所需的方法、培训和设施 成像是昂贵的，需要共享的核心设施，以促进创新的新的研究方法， 合作，以保持高生产力，并保持在该领域的最前沿。细胞成像和 形态分析核心提供了知识渊博的专家支持沿着明亮的视野， 在宏观水平（MVX 10）和微观水平（FV 500 & FV1200）。高度复杂的软件包（Metamorph和Imaris）使视觉研究人员能够 生成新的图像并通过以前不可用的软件对其进行分析。学生的培训， 博士后研究员，工作人员和PI在使用细胞成像核心设施大大扩展了他们的 调查能力，开发创造性的想法，并促进合作互动。