Eliminating the human factor from stereotaxic surgeries

消除立体定向手术中的人为因素

• 批准号: 10080673
• 负责人: Achim Klug
• 金额: $ 25.2万
• 依托单位: POPNEURON LLC
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10080673
• 关键词: 3-Dimensional; Animal Experimentation; Animal Experiments; Animals; Area; Atlases; Base of the Brain; Brain; Caliber; Computer Vision Systems; Computer software; Computers; Data; Databases; Devices; Dorsal; Electrodes; Elements; Ensure; Frustration; Goals; Human; Image; Injections; Intervention; Laboratories; Lighting; Location; MRI Scans; Magnetic Resonance Imaging; Manuals; Measurement; Measures; Mechanics; Monitor; Neuronavigation; Operative Surgical Procedures; Persons; Positioning Attribute; Procedures; Reporting; Reproducibility; Research; Research Personnel; Research Project Grants; Resolution; Robotics; Sampling; Savings; Scanning; Side; Site; Speed; Structure; Surgical sutures; System; Techniques; Technology; Time; Touch sensation; Translations; Vision; Work; X-Ray Computed Tomography; age group; base; bone; bone imaging; brain tissue; cloud based; cost effective; cranium; design; experimental study; genetic strain; hexapod; in vivo; laboratory experience; member; millimeter; novel; operation; optical fiber; programs; prototype; soft tissue; software development; tool; virtual; wasting

Project Summary: The main goal of this research project is to develop a new line of new stereotaxic devices for small animal research that outperforms existing devices in terms of accuracy, reproducibility, and ease of use. Advancing a tool such as an electrode, injection pipette or optical fiber through a small hole in the cranium, sometimes over long distances, and placing it precisely in a particular brain area, often much less than one millimeter in diameter, is a significant experimental challenge. Any time an investigator misses the target brain area and the experiment fails as a result, a significant amount of work is lost, additional animals get sacrificed, materials are wasted, and the pace of scientific discovery has been slowed. Even in cases when experiments succeed, they can be difficult to reproduce because many research groups rely on their most experienced lab members and their “special touch” to perform these procedures – thereby adding an element of non- quantitativeness to the procedures, effectively making the experiment less reproducible. We propose to develop a novel stereotaxic apparatus which will overcome many of these shortcomings. Our device features a radically different mechanical design which is natively compatible with both traditional and novel in-vivo techniques. We propose to combine computer 3D vision and robotics for automatic and software guided adjustments of the animal's skull. Landmarks are measured with 3D vision, based on structured illumination at a level of accuracy that has not been accomplished by any of the existing devices. This information will guide a robotic platform to position the animal for the experiment. Finally, we propose to develop an open software platform for neuronavigation that will allow investigators to use the platform with any small animal species they desire to use. Brain atlas systems for neuronavigation can either be downloaded from a cloud based site, or produced de-novo by the investigator by preparing a single set of MRI and CT scans from one sample animal. Our device will help make stereotaxic procedures more accurate and less dependent on human input and thereby increase the repeatability of experiments within a laboratory as well as the reproducibility of experiments across laboratories.

项目概要： 本研究项目的主要目标是开发一种新的小动物立体定位装置 在准确性、再现性和易用性方面优于现有设备的研究。推进 例如电极、注射吸管或光纤等工具穿过颅骨中的小孔，有时 并精确地将其放置在特定的大脑区域，通常小于一毫米 是一个重大的实验挑战。任何时候调查人员错过了目标大脑区域 结果实验失败，大量的工作损失，更多的动物被牺牲， 材料被浪费了，科学发现的速度被放慢了。即使在实验中 如果成功，它们可能很难复制，因为许多研究小组依赖于他们最有经验的实验室 成员和他们的“特殊触摸”来执行这些程序-从而增加了一个非- 程序的定量性，有效地降低了实验的可重复性。 我们建议开发一种新的立体定位装置，将克服许多这些缺点。我们 设备具有完全不同的机械设计，与传统的 和新的体内技术。我们建议将联合收割机计算机三维视觉和机器人技术相结合， 软件引导调整动物的头骨。地标采用3D视觉测量，基于 结构化照明的精确度水平还没有被任何现有设备实现。 这些信息将引导机器人平台定位动物进行实验。最后提出 开发一个开放的神经导航软件平台，允许研究人员使用该平台 任何他们想用的小动物种类。用于神经导航的脑图谱系统可以是 从基于云的网站下载，或由研究者通过准备一组 一只样本动物的MRI和CT扫描。 我们的设备将有助于使立体定位程序更准确，更少地依赖于人类输入， 从而提高实验室内实验的可重复性以及 跨实验室的实验