A Comprehensive Functional Map of Human Protein-RNA Interactions

人类蛋白质-RNA 相互作用的综合功能图谱

• 批准号: 10087952
• 负责人: Brenton R. Graveley
• 金额: $ 75.66万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-05 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10087952
• 关键词: Antibodies; Binding; Binding Sites; Biological Assay; Biology; Cell Line; Cells; Collection; Complement; Data; Elements; Encyclopedias; Epitopes; Foundations; Genes; Goals; Human; Human Biology; Human Cell Line; Human Genome; Immunofluorescence Immunologic; In Vitro; Knock-out; Maps; Measurement; Methods; Pattern; Post-Transcriptional Regulation; Production; Proteins; RNA; RNA Binding; RNA Sequences; RNA-Binding Proteins; RNA-Protein Interaction; Resources; Role; Specificity; Untranslated RNA; base; human disease; preference; transcriptome; transcriptome sequencing

PRODUCTION CORE – PROJECT SUMMARY The objective of the ENCORE (Encyclopedia of RNA Elements) project is to develop a foundational, functional map of protein-RNA interactions of RNA binding proteins (RBPs) encoded in the human genome, and the RNA elements they bind to across the transcriptome. These RNA elements, when expressed, form the basis of co- and post- transcriptional regulation of human genes. Our strategy consists of developing and integrating a physical map of 300 RBPs in two different human cell lines with transcriptome-wide measurements of the effects of depleting these RBPs, their localization patterns and their binding preferences independent of co-factor associations. In this data production core, we will capitalize on the highly efficient data production pipeline we have established over the past four years to generate RNA bind- n-seq, RNA-seq, protein localization and eCLIP data for an additional 300 RBPs. When combined with the data we generated over the past four years, these efforts will culminate in a comprehensive map of the functional RNA elements recognized by essentially all RBPs expressed in two human cell lines, representing approximately half of the known complement of human RBPs. In summary, the data we will produce in this project will enable a more systematic and comprehensive understanding of the role of RBPs and RNA biology in the contribution to human biology and disease.

生产核心-项目总结 ENCORE（Encyclopedia of RNA Elements）项目的目标是开发一个 RNA结合蛋白的蛋白质-RNA相互作用的基本功能图 在人类基因组中编码的RBP，以及它们在整个基因组中结合的RNA元件， 转录组这些RNA元件，当表达时，形成共-和后-的基础。 人类基因的转录调控。我们的战略包括发展和 整合两种不同人类细胞系中300个RBP的物理图谱， 在转录组范围内测量消耗这些限制性商业惯例的影响， 定位模式及其与辅因子无关的结合偏好 协会.在这个数据生产核心中，我们将利用高效的数据 我们在过去四年中建立了生产管道，以产生RNA结合- 另外300个RBP的n-seq、RNA-seq、蛋白定位和eCLIP数据。当 结合我们过去四年的数据，这些努力将 最终得到了一个由RNA识别的功能性RNA元件的综合图谱， 基本上所有的RBP都在两种人类细胞系中表达，占大约一半 已知的人类RBPs的互补物。总而言之，我们将在本报告中产生的数据 项目将使人们能够更系统和全面地了解 RBP和RNA生物学对人类生物学和疾病的贡献。