Very High Resolution Laboratory MicroCT Scanner

超高分辨率实验室 MicroCT 扫描仪

• 批准号: 7796469
• 负责人: D Rick Sumner
• 金额: $ 49.6万
• 依托单位: RUSH UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-24 至 2012-06-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7796469
• 关键词: 3-Dimensional; Academic Medical Centers; Animal Model; Architecture; Autopsy; Biochemical; Biological; Blood Vessels; Cartilage; Connective and Soft Tissue; Degenerative polyarthritis; Disease; Educational process of instructing; Equipment; Financial Support; Funding; Goals; Grant; Histology; Human; Inflammatory; Institution; Intestines; Investigation; Laboratories; Malignant Neoplasms; Marrow; Measurement; Mechanics; Minor; Mission; Osteoporosis; Permeability; Postdoctoral Fellow; Public Health; Replacement Arthroplasty; Research; Research Personnel; Research Training; Resolution; Rheumatoid Arthritis; Specimen; Stem cells; Structure; Students; Syndrome; Techniques; Tissue Engineering; Tissues; United States National Institutes of Health; Work; X-Ray Computed Tomography; angiogenesis; bone; cost; design; improved; injury and repair; instrument; instrumentation; medical schools; meetings; public health relevance; skeletal injury; trafficking; tumor

DESCRIPTION (provided by applicant): The goal of this project is to extend the capability of a multi-ser facility at Rush Medical College that will allow non-destructive, 3-dimensional measurement of tissue architecture through the use of very high resolution laboratory x-ray micro computed tomography. Bone, particular cartilage and other soft connective tissues as well as vascular tissues will be examined at approximately 4-fold improved resolution compared to current capabilities. This facility is designed to meet the needs of multiple investigators who are supported by funded NIH grants and who need the capabilities of the requested instrument in order to further the specific aims of their ongoing research. The approaches made possible in this new instrument will significantly extend their work beyond that which can be accomplished using techniques currently available. Extensive financial support is being provided by Rush Medical College as evidenced by an institutional commitment totaling $197,022 for dedicated laboratory space and partial operating costs for a period of 5 years. This facility will initially support the specific aims of 9 major users and 7 minor users, with a total of 13 NIH-funded grants. The projects are being conducted on the general topic of skeletal injury and repair, encompassing joint replacement, osteoarthritis, rheumatoid arthritis, tissue engineering; stem cell trafficking within the marrow microenvironment; and angiogenesis as related to tumors and the permeability of the gut lining. The investigations use animal models and human autopsy material. We anticipate being able to combine very sophisticated three- dimensional characterization of tissue architecture (0.5 <m isotropic voxels [2 <m resolution] as opposed to 6 <m isotropic voxels [9 <m resolution] with our current equipment) and to provide quicker turnaround for the nearly 5-fold increase in demand for our current equipment. In many cases, other information such as mechanical strength, biochemical composition, or histology can be collected from the same specimens since microCT is nondestructive. The new facility will also greatly enhance the research training of students, residents and post-doctoral fellows. Consequently, the facility is expected to make a major contribution to the research and teaching missions of Rush University Medical Center as well as collaborating institutions. PUBLIC HEALTH RELEVANCE: The requested instrumentation will provide a much improved means of assessing biological structures such as particular cartilage, bone and blood vessels in animal models. These measurements are critical to assess potential new treatments for diseases such as osteoarthritis, osteoporosis, rheumatoid arthritis, cancer and inflammatory bowel syndrome. The requested equipment is, therefore, relevant to public health.

描述（由申请人提供）：本项目的目标是扩展拉什医学院多服务器设施的能力，该设施将允许通过使用非常高分辨率的实验室X射线微计算机断层扫描对组织结构进行非破坏性三维测量。骨、特别是软骨和其他软结缔组织以及血管组织将以比现有能力提高约4倍的分辨率进行检查。该设施旨在满足多名研究人员的需求，这些研究人员由NIH资助赠款并且需要所需仪器的能力，以便进一步实现其正在进行的研究的特定目标。这一新文书所采用的方法将大大扩展他们的工作，使之超出使用现有技术所能完成的范围。拉什医学院正在提供广泛的财政支持，证明了一个机构的承诺总额为197,022美元的专用实验室空间和部分运营成本，为期5年。 该设施最初将支持9个主要用户和7个次要用户的具体目标，总共有13个NIH资助的赠款。这些项目的主题是骨骼损伤和修复，包括关节置换、骨关节炎、类风湿性关节炎、组织工程;骨髓微环境中的干细胞运输;以及与肿瘤和肠内膜渗透性有关的血管生成。研究使用动物模型和人类尸检材料。我们期望能够将组织结构的非常复杂的三维表征（0.5 μ m各向同性体素[2 μ m分辨率]与我们当前的设备的6 μ m各向同性体素[9 μ m分辨率]相对）结合联合收割机，并为我们当前设备的需求增加近5倍提供更快的周转。在许多情况下，其他信息，如机械强度，生化成分，或组织学可以从相同的标本收集，因为microCT是无损的。 新设施还将大大加强学生，居民和博士后研究员的研究培训。因此，该设施有望为拉什大学医学中心以及合作机构的研究和教学任务做出重大贡献。 公共卫生相关性：所要求的仪器将提供一种大大改进的评估生物结构的手段，例如动物模型中的特定软骨、骨和血管。这些测量对于评估骨关节炎、骨质疏松症、类风湿性关节炎、癌症和炎症性肠综合征等疾病的潜在新疗法至关重要。因此，所要求的设备与公共卫生有关。

项目成果

Optimizing a micro-computed tomography-based surrogate measurement of bone-implant contact.

• DOI: 10.1002/jor.23716
• 发表时间: 2018-03
• 期刊: Journal of orthopaedic research : official publication of the Orthopaedic Research Society
• 作者: Meagher MJ;Parwani RN;Virdi AS;Sumner DR
• 通讯作者: Sumner DR

Bone Matrix Maturation in a Rat Model of Intra-Cortical Bone Remodeling.

皮质内骨重塑大鼠模型中的骨基质成熟。

• DOI: 10.1007/s00223-017-0270-7
• 发表时间: 2017
• 期刊: Calcified tissue international
• 影响因子: 4.2
• 作者: Ross,RyanD;Sumner,DRick
• 通讯作者: Sumner,DRick