Temperature Controlled Rodent Activity, Feeding and Indirect Calorimeter System

温控啮齿动物活动、喂食和间接热量计系统

• 批准号: 7794110
• 负责人: KC KENT LLOYD
• 金额: $ 49.2万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-11 至 2011-03-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794110
• 关键词: Adipose tissue; Animal Model; Area; Award; Behavior; Biology; Body Composition; Carbohydrates; Carbon Dioxide; Complement; Devices; Diabetes Mellitus; Eating; Energy Metabolism; Equilibrium; Equipment; Evaluation; Exercise; Exercise Physiology; Faculty; Fatty acid glycerol esters; Frequencies; Functional disorder; Funding; Grant; Home environment; Homeostasis; Individual; Intake; Knowledge; Liver; Macronutrients Nutrition; Measurement; Measures; Metabolic; Metabolic syndrome; Minor; Mission; Modeling; Mus; Muscle; Names; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Output; Phenotype; Physical activity; Proteins; Public Health; Rattus; Regulation; Research; Research Personnel; Risk; Rodent Control; Site; System; Temperature; Time; Tissues; United States National Institutes of Health; Weight; Whole Organism; base; energy balance; feeding; mouse model; nutrition; programs; respiratory gas

DESCRIPTION (provided by applicant): Measurement of the individual factors that drive energy balance and tissue macronutrient fuel choice is fundamental to understanding the mechanisms influencing food intake behavior, energy expenditure, and ultimately, weight gain and body composition. Such knowledge is directly relevant to pressing public health issues such as obesity, type 2 diabetes mellitus, metabolic syndrome, and others for which risk is associated with metabolic dysfunction and long-term positive energy balance that results in excessive calorie storage as fat (in adipose tissue, liver, and other sites). To fully evaluate the complexities of energetics and regulation of metabolic homeostasis, animal models are frequently employed since energetics measurements can be correlated to intensive evaluations of biology from the whole-organism down to tissue and metabolite levels. Optimally, studies using these models should include measures of a variety of factors that influence net calorie balance and the utilization of carbohydrate, fat, or protein for energy, including, e.g., (a) calorie intake rates and meal frequencies, (b) energy expenditure and behavior- activity levels that contribute to calorie demands, and (c) respiratory gas exchange ratios (CO2 output vs. O2 intake) to calculate whole-body tissue macronutrient fuel choice. In this application, we propose to complement existing UC Davis campus strengths in diabetes and obesity research, exercise physiology and muscle biology, and nutrition and energetics through the purchase of a multi-user device with comprehensive capabilities to simultaneously and automatically record food intake/meal frequencies, activity levels, and metabolic rate and fuel choice. The latter can be determined in a home cage, in the course of physical activity shifts or exercise, or with changes in environmental temperature. This unit has the advantage of being able to accommodate large numbers of mice or rats, thus enabling individual investigators to complete statistically-robust studies in relatively short periods of time, and allowing for broader-based metabolic phenotyping efforts in genetically-modified mouse models in support of the UC Davis Mouse Biology Program mission area. Nine NIH funded faculty with 14 grants active at the time of award are named major and minor users of this equipment.

描述（由申请人提供）：测量驱动能量平衡和组织宏量营养素燃料选择的个体因素对于理解影响食物摄入行为、能量消耗以及最终体重增加和身体组成的机制至关重要。这些知识与紧迫的公共卫生问题直接相关，如肥胖症，2型糖尿病，代谢综合征，以及其他风险与代谢功能障碍和长期正能量平衡相关的问题，导致过量的卡路里储存为脂肪（在脂肪组织，肝脏和其他部位）。为了充分评估能量学和代谢稳态调节的复杂性，经常采用动物模型，因为能量学测量可以与从整个生物体到组织和代谢物水平的生物学的深入评价相关。最理想的情况是，使用这些模型的研究应包括对影响净卡路里平衡和碳水化合物、脂肪或蛋白质能量利用的各种因素的测量，包括，例如，(a)卡路里摄入率和进餐频率，（B）能量消耗和有助于卡路里需求的行为活动水平，以及（c）呼吸气体交换比（CO2输出与O2摄入），以计算全身组织大量营养素燃料选择。在此应用中，我们建议通过购买具有全面功能的多用户设备来补充现有的加州大学戴维斯分校在糖尿病和肥胖研究，运动生理学和肌肉生物学以及营养和能量学方面的优势，以同时自动记录食物摄入/用餐频率，活动水平，代谢率和燃料选择。后者可以在笼子里，在身体活动转变或锻炼的过程中，或随着环境温度的变化而确定。该单位的优势是能够容纳大量的小鼠或大鼠，从而使个别研究人员能够在相对较短的时间内完成药理学稳健的研究，并允许在基因修饰小鼠模型中进行更广泛的代谢表型分析，以支持加州大学戴维斯分校小鼠生物学计划使命领域。九个NIH资助的教师与14个赠款活动在授予时被命名为该设备的主要和次要用户。