In Vivo Model and Histology Core

体内模型和组织学核心

• 批准号: 10090195
• 负责人: Gehua Zhen
• 金额: $ 49.91万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-15 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10090195
• 关键词: 3-Dimensional; Aging; Animal Model; Animals; Behavior; Biomechanics; Blinded; Cartilage; Categories; Charge; Classification; Clinical; Continuing Education; Data; Data Analyses; Degenerative Disorder; Degenerative polyarthritis; Ensure; Environment; Equipment; Evaluation; Fast Green; Functional disorder; Goals; Grouping; Histologic; Histology; Histopathologic Grade; Histopathology; Human Resources; Hyperalgesia; Image Analysis; Individual; International; Intervertebral disc structure; Investigation; Joints; Knee joint; Laboratories; Laboratory Personnel; Measurement; Mechanics; Medial meniscus structure; Methods; Microsurgery; Modeling; Morphology; Operative Surgical Procedures; Organism; Outcome; Pain; Pathogenicity; Pathologic; Persons; Procedures; Program Research Project Grants; Protocols documentation; Reproducibility; Reproducibility of Results; Research; Research Personnel; Resources; Running; Scanning; Services; Severities; Skeleton; Societies; Specialist; Spinal; Spinal Ganglia; Stains; Standardization; Supervision; Symptoms; System; Testing; Therapeutic; Time; Tissues; Training; Translating; Variant; Vascularization; Vertebral column; age related; articular cartilage; base; behavior test; bone; conditioned place preference; cost; design; drug candidate; experience; experimental study; gait examination; in vivo; in vivo Model; in vivo imaging; insight; joint destruction; lumbar vertebra bone structure; member; microCT; mouse model; multidisciplinary; operation; physically handicapped; pre-clinical; programs; skeletal; spine bone structure; subchondral bone; tissue preparation; tomography

PROJECT SUMMARY The purpose of the in vivo Animal model & histology core is to consolidate key personnel and equipment to provide a centralized facility that will enhance collaborative and multidisciplinary investigations into multiple mechanisms in age related skeletal degenerative disease. Core B will serve as a center to realize an optimized and reasonable utilization and allocation of experimental and human resources. Employing skillful personnel to conduct the common procedures in a standardized manner is not only a cost- and time-efficient strategy but also can maximally reduce the potential for bias and increase the scientific rigor. Ranging from tissue histological analysis to functional behavior test, Core B will facilitate the investigation of age related skeletal degenerative diseases from four aspects: 1. Animal models and microsurgeries. The proposed surgical animal models: destabilization of the medial meniscus (DMM), lumbar spine instability (LSI), dorsal root ganglion (DRG) exposure for in vivo imaging will be conducted in the Core B. Our core will set up a team with members who have extensive experience in conducting complicated microsurgeries in small animals. 2. Histological grading. Core B will perform histological grading based on the Safranin O/fast green and H&E staining. The osteoarthritis research society international (OARSI) scoring system will be applied to assess the joint degenerative status while the grading method for classification of age-related changes in lumbar intervertebral discs will be used to determine the degenerative status of lumbar discs. 3. Micro-CT analysis. Micro-CT measurements will be done in excised lumbar vertebrae and knee joints to determine the individual volumetric, densitometric and 3- dimensional microarchitecture of subchondral bone or endplate. These parameters are essential for investigating the biomechanical impact of subchondral bone on articular cartilage or endplate on intra-vertebral discs. 4: Behavior test. To better translate the preclinical results into clinical settings, behavior test will be applied to all the 4 projects within this PPG program. Core B will perform gait analysis, Von Frey test, Mechanical hyperalgesia and cold sensitivity test for individual projects. To reduce the variability that potentially generated because of the changes of environment, the different handling of animals, Core B will designate well trained personnel in charging of the animal behavioral tests and equipment/facility management. 5. Training. The Core will train and maintain the continuing education of a key laboratory person from each project to help perform the routine histological, behavior and micro-CT analysis.

项目总结 活体动物模型和组织学核心的目的是巩固关键人员和设备，以 提供集中式设施，可加强对多个领域的协作调查 年龄相关性骨骼退行性疾病的发病机制。核心B将作为中心，实现优化的 合理利用和配置实验和人力资源。聘请熟练的人员来 以标准化的方式执行共同程序不仅是一种节省成本和时间的策略，而且还 可以最大限度地减少偏见的可能性，并提高科学的严谨性。从组织组织学到 功能行为测试分析，核心B将有助于年龄相关性骨骼退行性变的研究 疾病从四个方面：1。动物模型和显微手术。建议的外科动物模型： 内侧半月板不稳(DMM)、腰椎不稳(LSI)、背根神经节(DRG) 活体成像的曝光将在核心B进行。我们的核心将成立一个团队，成员包括 在小动物身上进行复杂的显微手术方面有丰富的经验。2.组织学分级。 Core B将根据藏红花O/坚固绿和H&E染色进行组织学分级。骨性关节炎 将应用国际研究学会(OARSI)评分系统来评估关节退变状况 而腰椎间盘增龄性改变的分级方法将用于 确定腰椎间盘退变状态。3.显微CT分析。将进行Micro-CT测量 在切除的腰椎和膝关节中测定个体的体积、密度和3- 软骨下骨或终板的三维微结构。这些参数对于调查是必不可少的 软骨下骨对关节软骨或椎间盘终板的生物力学影响。4： 行为测试。为了更好地将临床前结果转化为临床环境，将对所有人进行行为测试 这个PPG计划中的4个项目。核心B将执行步态分析、冯·弗雷测试、机械性痛觉过敏 以及个别项目的冷敏试验。以减少可能由于 环境的变化，动物的不同处理，核心B将指定训练有素的人员 负责动物行为测试和设备/设施管理。5.培训。核心将训练和 对每个项目中的关键实验室人员进行继续教育，以帮助执行常规工作 组织学、行为学和显微CT分析。