Proteomics and Metabolomics Shared Resource

蛋白质组学和代谢组学共享资源

基本信息

  • 批准号:
    10570946
  • 负责人:
  • 金额:
    $ 20.66万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1997
  • 资助国家:
    美国
  • 起止时间:
    1997-04-01 至 2024-02-29
  • 项目状态:
    已结题

项目摘要

PROJECT SUMMARY – PROTEOMICS AND METABOLOMICS SHARED RESOURCE The primary goal of the Proteomics and Metabolomics Shared Resource (PMSR) is to provide comprehensive proteomics and metabolomics services to Cancer Center members as well as other regional NCI-designated Cancer Centers (Fox Chase Cancer Center, Thomas Jefferson University Cancer Center), and secondarily to investigators in other academic institutions. Prior to December 2017, major mass spectrometry (MS) instruments in the PMSR included a Thermo Q Exactive HF, a Thermo Q Exactive Plus, and a Sciex QTRAP 5500 hybrid triple quadrupole mass spectrometer. These instruments have nano-UHPLCs (ultra-high pressure liquid chromatography) with cooled-autosamplers on the front end for proteomic analyses. A conventional flow UHPLC is used for metabolite analyses. These LC-MS systems represent some of the highest performing, most sensitive instruments currently available. The PMSR will continue to offer routine proteomic services such as LC-MS/MS protein identifications from gel bands or pull-down assays, and intact protein analysis by electrospray ionization. Over the last budget cycle, the PMSR has increasingly engaged in more challenging proteomics problems, particularly global quantitative comparisons of proteomes or sub-proteomes using label- free quantitation (integrated MS ion currents), quantitation using stable isotopes such as SILAC or isobaric tags (TMT or iTRAQ), and in-depth global analysis of phosphoproteome, ubiquitomes, and acetylomes. The PMSR will continue to improve its analytical capabilities for in-depth comprehensive analysis of global proteome and posttranslational modifications. The PMSR has also responded to the growing need of Cancer Center members in the field of cancer metabolism by establishing targeted relative quantitation of approximately 180 polar metabolites. To further increase its capacity in metabolomics analysis, the PMSR applied for and was recently awarded an S10 shared instrumentation grant (S10 OD023586). This grant together with Cancer Center funds were used to purchase a high-resolution Thermo Q Exactive HF-X mass spectrometer and a Vanquish UHPLC system. This instrument was installed in December 2017 and will be dedicated to untargeted (global) metabolomics, targeted metabolomics, untargeted lipidomics, targeted lipidomics and metabolic flux analysis using stable isotope tracers. The PMSR also recently purchased a Seahorse XFe96 Analyzer funded by another S10 grant (S10 OD023658). This instrument can quantify mitochondrial respiration, glycolytic activity, endogenous and exogenous fatty acid oxidation, substrate oxidation, and metabolic phenotype, providing complementary analytical capacity to the new metabolomics LC-MS/MS system. The availability of these new instruments will ensure that the PMSR remains state-of-the-art in providing proteomics, metabolomics, and lipidomics services to Cancer Center members. The major emphasis of the PMSR will be to provide expert consultation and state-of-the-art proteomics and metabolomics technologies operating at maximum performance and affordable costs to address complex cancer biology questions.
项目概要-蛋白质组学和代谢学共享资源 蛋白质组学和代谢组学共享资源(PMSR)的主要目标是提供全面的 蛋白质组学和代谢组学服务,以癌症中心成员以及其他地区的国家癌症研究所指定 癌症中心(福克斯蔡斯癌症中心,托马斯杰斐逊大学癌症中心),其次是 其他学术机构的研究人员。在2017年12月之前,主要的质谱(MS) PMSR中的仪器包括Thermo Q Exactive HF、Thermo Q Exactive Plus和Sciex QTRAP 5500混合三重四极杆质谱仪。这些仪器具有纳米UHPLC(超高压 液相色谱法),在前端具有冷却的自动进样器,用于蛋白质组学分析。常规流 UHPLC用于代谢物分析。这些LC-MS系统代表了一些最高性能, 目前最敏感的仪器。PMSR将继续提供常规蛋白质组学服务, 作为凝胶带或下拉试验的LC-MS/MS蛋白质鉴定,以及通过 电喷雾电离在上一个预算周期,PMSR越来越多地参与更具挑战性的 蛋白质组学问题,特别是使用标签的蛋白质组或亚蛋白质组的全球定量比较, 自由定量(积分MS离子流),使用稳定同位素(如SILAC或同量异位素标签)定量 (TMT或iTRAQ),以及对磷酸化蛋白质组、泛素组和乙酰基组的深入全球分析。PMSR 将继续提高其分析能力,对全球蛋白质组进行深入全面的分析, 翻译后修饰PMSR还响应了癌症中心日益增长的需求, 癌症代谢领域的成员通过建立约180 极性代谢物。为了进一步提高其代谢组学分析能力,PMSR申请并 最近获得了S10共享仪器补助金(S10 OD 023586)。这份补助金与癌症一起 中心资金用于购买高分辨率Thermo Q Exactive HF-X质谱仪和 Vanquish UHPLC系统。该仪器于2017年12月安装,将专门用于非目标 (全局)代谢组学、靶向代谢组学、非靶向脂质组学、靶向脂质组学和代谢通量 使用稳定同位素示踪剂进行分析。PMSR最近还购买了Seahorse XFe 96分析仪, 通过另一个S10授权(S10 OD 023658)。该仪器可以定量线粒体呼吸、糖酵解 活性、内源性和外源性脂肪酸氧化、底物氧化和代谢表型, 为新的代谢组学LC-MS/MS系统提供补充分析能力。的可用性 这些新仪器将确保PMSR在提供蛋白质组学方面保持最新水平, 为癌症中心成员提供代谢组学和脂质组学服务。PMSR的主要重点是 提供专家咨询和最先进的蛋白质组学和代谢组学技术, 最大的性能和可承受的成本来解决复杂的癌症生物学问题。

项目成果

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DAVID W. SPEICHER其他文献

DAVID W. SPEICHER的其他文献

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{{ truncateString('DAVID W. SPEICHER', 18)}}的其他基金

Purchase of a Q Exactive HF mass spectrometer system for metabolomics
购买用于代谢组学的 Q Exactive HF 质谱仪系统
  • 批准号:
    9274622
  • 财政年份:
    2017
  • 资助金额:
    $ 20.66万
  • 项目类别:
Proteomics Facility
蛋白质组学设施
  • 批准号:
    7945027
  • 财政年份:
    2009
  • 资助金额:
    $ 20.66万
  • 项目类别:
Identification of ovarian cancer plasma biomarkers
卵巢癌血浆生物标志物的鉴定
  • 批准号:
    7736151
  • 财政年份:
    2009
  • 资助金额:
    $ 20.66万
  • 项目类别:
Identification of Ovarian Cancer Plasma Biomarkers
卵巢癌血浆生物标志物的鉴定
  • 批准号:
    8759302
  • 财政年份:
    2009
  • 资助金额:
    $ 20.66万
  • 项目类别:
Identification of ovarian cancer plasma biomarkers
卵巢癌血浆生物标志物的鉴定
  • 批准号:
    8192927
  • 财政年份:
    2009
  • 资助金额:
    $ 20.66万
  • 项目类别:
Identification of ovarian cancer plasma biomarkers
卵巢癌血浆生物标志物的鉴定
  • 批准号:
    8296102
  • 财政年份:
    2009
  • 资助金额:
    $ 20.66万
  • 项目类别:
Protein Modification with Oxidative Stress in ALI
ALI 中氧化应激的蛋白质修饰
  • 批准号:
    7796691
  • 财政年份:
    2009
  • 资助金额:
    $ 20.66万
  • 项目类别:
Identification of ovarian cancer plasma biomarkers
卵巢癌血浆生物标志物的鉴定
  • 批准号:
    7881494
  • 财政年份:
    2009
  • 资助金额:
    $ 20.66万
  • 项目类别:
Identification of Ovarian Cancer Plasma Biomarkers
卵巢癌血浆生物标志物的鉴定
  • 批准号:
    8910661
  • 财政年份:
    2009
  • 资助金额:
    $ 20.66万
  • 项目类别:
Purchase of an ion trap mass spectrometer
购买离子阱质谱仪
  • 批准号:
    7217102
  • 财政年份:
    2007
  • 资助金额:
    $ 20.66万
  • 项目类别:

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