Administrative supplement for the flow cytometry cell sorter purchase

南卡罗来纳大学科布雷靶向治疗中心(CTT)支持 寻求开发针对各种疾病的新的、更有效的药物类别的研究，通过 这些药物针对的是在疾病中发挥关键作用的分子和细胞靶点。FACS是一种强有力的方法 收集和分析药物治疗后的各种细胞群。重要的是，这些细胞可以来自 从动物模型中分离出的组织或血液。然后可以对分类的种群进行进一步的 分析，如基因表达或免疫细胞标记物分类。CTT中现有的细胞分选机 显微镜和流式细胞仪核心(MFCC)已经使用了19年，有几个局限性 相对于更现代的仪器设备。更改输入像元源时很难不引起 污染，从分离的样品几乎总是污染仪器。去污是 这很困难，而且正在削弱旧的射流系统。这项建议的目的是获得资金，以购买一个 索尼SH800细胞分选机。该仪器采用一次性微流体芯片，消除了交叉污染。 较旧的仪器遇到问题。此外，它的操作要容易得多，与之相比，对细胞进行分类的速度也更快 给上一代人。已经有几名调查人员对此表示支持 申请。它们的应用包括在乳腺动物模型中表征浸润性免疫细胞 癌症；从发育中的斑马鱼中分离细胞以了解颅面发育的机制；分选 抗生素致小鼠早发性结直肠癌模型中结肠微生物区系和上皮细胞的变化 目前的CTT目标项目是收集造血干细胞，用于移植研究。 将这一仪器加入MFCC将扩大核心的能力，并帮助CTT实现 它的目标是为开发靶向疗法的研究人员提供所需的资源。

The COBRE Center for Targeted Therapeutics (CTT) at the University of South Carolina (UofSC) supports research that seeks to develop new and more effective classes of drugs against various diseases, by aiming these drugs at molecular and cellular targets that play a key role in the disease. FACS is a powerful method to collect and analyze various populations of cells after a drug treatment. Importantly, the cells can be from dissociated tissue or blood from animal models. The sorted populations can then be subjected to further analysis, such as gene expression or immune cell marker classification. The existing cell sorter in the CTT Microscopy and Flow Cytometry Core (MFCC) has been in service for 19 years and has several limitations relative to more modern instrumentation. It is difficult to change the source of input cells without causing contamination, and samples from dissociated almost always contaminates the instrument. Decontamination is difficult and is weakening the old fluidics system. The purpose of this proposal is to acquire funds to purchase a Sony SH800 Cell Sorter. This instrument uses disposable microfluids chips to eliminate the cross-contamination problem experienced with the older instrument. Also, it is much easier to operate and sorts cells faster compared to the previous generation. There are several investigators who have already expressed their support for this application. Their applications include characterizing infiltrating immune cells in an animal model of breast cancer; sorting cells from developing zebrafish to understand mechanisms of craniofacial development; sorting the microbiota and epithelial cells of colon in a mouse model of early onset colorectal cancer caused by antibiotic treatment; and a current CTT-target project, collect hematopoietic stem cells to use in transplantation studies. The addition of this instrument to the MFCC will expand the capability of the core and help the CTT to achieve its goals to provide needed resources to investigators developing targeted therapeutics.