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The Biophysics Collaborative Access Team

生物物理学协作访问团队

基本信息

批准号：
10032571
负责人：
THOMAS C IRVING
金额：
$ 375.3万
依托单位：
ILLINOIS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-02-01 至 2025-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10032571
关键词：
Address Advisory Committees Aging Amyloid Automation Biochemical Biocompatible Materials Biological Biology Biophysics Biotechnology Cardiomyopathies Cell physiology Cells Charge Collaborations Communities Complex Computer software Connective Tissue Data Data Analyses Development Disease model Drug Design Educational workshop Ensure Equilibrium Equipment Evolution Fiber Funding Grant Human Pathology Illinois Image Individual Infrastructure Institutes Instruction Laboratories Leadership Ligands Light Maintenance Mission Modification Molecular Molecular Biology Molecular Sieve Chromatography Muscle Muscle Fibers Myocardium National Center for Research Resources National Institute of General Medical Sciences Neoplasm Metastasis Noise Nucleic Acid Folding Nucleic Acids Pathology Persons Photons Physics Physiological Policies Principal Investigator Productivity Proteins Publications Radiation induced damage Recruitment Activity Research Research Personnel Research Project Grants Research Technics Resources Sampling Scanning Schedule Science Shapes Signal Transduction Site Skeletal Muscle Source Structure Students Synchrotrons System Technology Time Tissue Sample Training Training Activity Traumatic Brain Injury United States National Institutes of Health Update Visit X ray diffraction analysis base beamline biological systems biophysical properties computerized data processing data acquisition data quality experience experimental study fibrous protein improved inherited cardiomyopathy instrumentation interest macromolecule operation outreach prevent professor programs protein structure repaired structural biology symposium synchrotron radiation tool training opportunity user-friendly web site

项目摘要

The mission of the Biophysics Collaborative Access Team (BioCAT) is to provide user access to a state-of-the- art synchrotron beamline (18ID) for diffraction and scattering of non-crystalline biological materials at the Advanced Photon Source, Argonne National Lab. Continued evolution of the beamline since first light in 1997 has resulted in a unique in the USA fiber diffraction program, and a world-class Bio-SAXS program with unique capabilities both in the equilibrium and time-resolved realms. The use of size exclusion chromatography in line with the SAXS camera has ensured unprecedentedly high data quality even with biochemically challenging samples otherwise thought to be intractable for SAXS. The recent incorporation of MALS and DLS in line with SEC allows additional biophysical characterization that can be invaluable in interpreting SAXS data from difficult systems. Our new coflow sheath flow sample cell has revolutionized SAXS data acquisition by preventing radiation damage, enhancing efficiency accompanied by significant increases in signal to noise. BioCAT maintains specialized apparatuses for combined time-resolved X-ray diffraction and physiological studies of cardiac and skeletal muscle. These studies have extended in scope from basic biophysical problems to address new disease models including cardiomyopathies. A traditional strength is fiber diffraction of general fibrous protein systems that has recently expanded into scanning diffraction imaging of fibrous protein structures in complex tissue samples. A major strength of the resource is the experienced and dedicated scientific staff who assist users seven days a week in data acquisition, processing, analysis and interpretation. BioCAT is directed by Dr. Thomas Irving, who has more than 20 years of experience overseeing synchrotron facilities for non-crystalline diffraction and scattering. Dr. Jesse Hopkins oversaw installation of the new coflow SAXS sample cell, established new user-friendly GUI based data acquisition interfaces, and provide advanced tools to interpret SEC-SAXS data. Dr. Hopkins develops and maintains the BioXTAS RAW SAXS data processing and analysis software package and will extend it to allow increased automation to optimize productivity while minimizing on-site effort for users. Dr. Srinivas Chakravarthy BioCAT’s resident biochemist coordinates the SAXS user program, assists users with conducting experiments on site, and also maintains the biochemical infrastructure. Dr. Weikang Ma is an expert in fiber diffraction of muscle and is responsible for providing comprehensive support to all our fiber diffraction users. All four of these individuals are involved in training and dissemination activities, including scientific publications, presentations at conferences, organization of BioCAT workshops and participating in community organized workshops. BioCAT has an updated website with a comprehensive set of instructions for all aspects of the resource that are sufficient for a user to plan and execute successful experiments at the beamline. Together the SAXS and Fiber Diffraction user programs host 70-90 user groups per year and consistently produce 30-40 publications per year.

生物物理协作访问小组（BioCAT）的任务是为用户提供对状态的访问