The Biophysics Collaborative Access Team

生物物理学协作访问团队

• 批准号: 10032571
• 负责人: THOMAS C IRVING
• 金额: $ 375.3万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10032571
• 关键词: Address; Advisory Committees; Aging; Amyloid; Automation; Biochemical; Biocompatible Materials; Biological; Biology; Biophysics; Biotechnology; Cardiomyopathies; Cell physiology; Cells; Charge; Collaborations; Communities; Complex; Computer software; Connective Tissue; Data; Data Analyses; Development; Disease model; Drug Design; Educational workshop; Ensure; Equilibrium; Equipment; Evolution; Fiber; Funding; Grant; Human Pathology; Illinois; Image; Individual; Infrastructure; Institutes; Instruction; Laboratories; Leadership; Ligands; Light; Maintenance; Mission; Modification; Molecular; Molecular Biology; Molecular Sieve Chromatography; Muscle; Muscle Fibers; Myocardium; National Center for Research Resources; National Institute of General Medical Sciences; Neoplasm Metastasis; Noise; Nucleic Acid Folding; Nucleic Acids; Pathology; Persons; Photons; Physics; Physiological; Policies; Principal Investigator; Productivity; Proteins; Publications; Radiation induced damage; Recruitment Activity; Research; Research Personnel; Research Project Grants; Research Technics; Resources; Sampling; Scanning; Schedule; Science; Shapes; Signal Transduction; Site; Skeletal Muscle; Source; Structure; Students; Synchrotrons; System; Technology; Time; Tissue Sample; Training; Training Activity; Traumatic Brain Injury; United States National Institutes of Health; Update; Visit; X ray diffraction analysis; base; beamline; biological systems; biophysical properties; computerized data processing; data acquisition; data quality; experience; experimental study; fibrous protein; improved; inherited cardiomyopathy; instrumentation; interest; macromolecule; operation; outreach; prevent; professor; programs; protein structure; repaired; structural biology; symposium; synchrotron radiation; tool; training opportunity; user-friendly; web site

The mission of the Biophysics Collaborative Access Team (BioCAT) is to provide user access to a state-of-the- art synchrotron beamline (18ID) for diffraction and scattering of non-crystalline biological materials at the Advanced Photon Source, Argonne National Lab. Continued evolution of the beamline since first light in 1997 has resulted in a unique in the USA fiber diffraction program, and a world-class Bio-SAXS program with unique capabilities both in the equilibrium and time-resolved realms. The use of size exclusion chromatography in line with the SAXS camera has ensured unprecedentedly high data quality even with biochemically challenging samples otherwise thought to be intractable for SAXS. The recent incorporation of MALS and DLS in line with SEC allows additional biophysical characterization that can be invaluable in interpreting SAXS data from difficult systems. Our new coflow sheath flow sample cell has revolutionized SAXS data acquisition by preventing radiation damage, enhancing efficiency accompanied by significant increases in signal to noise. BioCAT maintains specialized apparatuses for combined time-resolved X-ray diffraction and physiological studies of cardiac and skeletal muscle. These studies have extended in scope from basic biophysical problems to address new disease models including cardiomyopathies. A traditional strength is fiber diffraction of general fibrous protein systems that has recently expanded into scanning diffraction imaging of fibrous protein structures in complex tissue samples. A major strength of the resource is the experienced and dedicated scientific staff who assist users seven days a week in data acquisition, processing, analysis and interpretation. BioCAT is directed by Dr. Thomas Irving, who has more than 20 years of experience overseeing synchrotron facilities for non-crystalline diffraction and scattering. Dr. Jesse Hopkins oversaw installation of the new coflow SAXS sample cell, established new user-friendly GUI based data acquisition interfaces, and provide advanced tools to interpret SEC-SAXS data. Dr. Hopkins develops and maintains the BioXTAS RAW SAXS data processing and analysis software package and will extend it to allow increased automation to optimize productivity while minimizing on-site effort for users. Dr. Srinivas Chakravarthy BioCAT’s resident biochemist coordinates the SAXS user program, assists users with conducting experiments on site, and also maintains the biochemical infrastructure. Dr. Weikang Ma is an expert in fiber diffraction of muscle and is responsible for providing comprehensive support to all our fiber diffraction users. All four of these individuals are involved in training and dissemination activities, including scientific publications, presentations at conferences, organization of BioCAT workshops and participating in community organized workshops. BioCAT has an updated website with a comprehensive set of instructions for all aspects of the resource that are sufficient for a user to plan and execute successful experiments at the beamline. Together the SAXS and Fiber Diffraction user programs host 70-90 user groups per year and consistently produce 30-40 publications per year.

生物物理协作访问团队(BioCAT)的任务是为用户提供对最新状态的访问 ART同步加速器光束线(18ID)，用于非晶态生物材料的衍射和散射 高级光子源，阿贡国家实验室。自1997年第一道亮光以来光束线的持续演变 已经产生了在美国独一无二的光纤衍射计划，以及具有独一无二的世界级Bio-SAXS计划 在平衡领域和时间分辨领域的能力。体积排阻层析法在联用中的应用 使用SAXS相机确保了前所未有的高数据质量，即使在生物化学方面具有挑战性 其他情况下被认为对SAXS很难处理的样本。最近合并了MAL和DLS，以符合 SEC允许额外的生物物理表征，这在解释SAXS数据方面可能是无价的 复杂的系统。我们新的同流鞘流样品池通过以下方式彻底改变了SAXS数据采集 防止辐射损伤，提高效率，同时显著提高信噪比。 BioCAT拥有结合时间分辨X射线衍射和生理学的专门仪器 研究心肌和骨骼肌。这些研究的范围从基本的生物物理问题扩展到 以应对包括心肌病在内的新疾病模型。一种传统的强度是一般的纤维衍射。 最近扩展到纤维蛋白扫描衍射成像的纤维蛋白系统 复杂组织样本中的结构。资源的一个主要优势是经验丰富和敬业的 每周七天协助用户获取、处理、分析和解释数据的科学工作人员。 BioCAT由Thomas Irving博士指导，他有20多年监管同步加速器的经验 用于非晶态衍射和散射的设备。杰西·霍普金斯博士监督安装新的Coflow SAXS样品池，建立了新的基于用户友好的图形用户界面的数据采集界面，并提供了先进的 用于解释SEC-SAXS数据的工具。霍普金斯博士开发和维护BioXTAS原始SAXS数据 处理和分析软件包，并将对其进行扩展，以提高自动化程度以优化 提高工作效率，同时最大限度地减少用户的现场工作量。生物猫的常驻生物化学家斯里尼瓦斯·查克拉瓦西博士 协调SAXS用户程序，协助用户进行现场实验，并维护 生化基础设施。马伟康博士是肌肉纤维衍射方面的专家，负责 为我们所有的光纤衍射用户提供全面的支持。这四个人都参与了 培训和传播活动，包括科学出版物、在会议上的发言、 组织生物CAT讲习班，并参加社区组织的讲习班。BioCAT有一个 更新的网站，其中包含资源所有方面的全面说明，这些说明足以满足 用户可以在光束线上计划和执行成功的实验。小角X射线衍射与光纤衍射的结合 用户计划每年拥有70-90个用户组，每年持续出版30-40份出版物。