Hybrid Rat Diversity Program - cryopreservation resource for rat models

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Project Summary The ongoing goal of this program is to extend the use of rat models for clinical translation through establishing an animal resource and genomic dataset to facilitate the translation of laboratory research to clinical relevance. Specifically, the Hybrid Rat Diversity Panel (HRDP) resource provides the research community with a foundation for systems genetics and a tool for forward genetics. The HRDP parent award aims to 1) to continue to establish the 96 strain Hybrid Rat Diversity Panel comprised of 33 classic inbred strains, 30 HXB/BXH recombinant inbred strains and 33 FXLE/LEXF recombinant inbred strains through embryo rederivation and importation, 2) to characterize, maintain and distribute the HRDP, 3) to provide genomic and transcriptomic sequencing of the HRDP and 4) and provide data analysis, integration and dissemination primarily through the Rat Genome Database (RGD). Specifically, this supplement will 1) evaluate embryo quality pre- and post-cryopreservation, 2) improve the efficiency of embryo collection for cryopreservation, and 3) develop cryopreservation protocols customized to strain characteristics. Together these results will have broad utility the scientific community through the genetic and phenotypic diversity represented in the strains selected for the supplement project in combination with the available data from the HRDP parent award. Data and protocols will be made available to the research community through the Rat Genome Database, publications, and through rat repositories.