Hybrid Rat Diversity Program - cryopreservation resource for rat models

杂交大鼠多样性计划 - 大鼠模型的冷冻保存资源

• 批准号: 10609632
• 负责人: MELINDA R DWINELL
• 金额: $ 20.34万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2026-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10609632
• 关键词: Age; Award; Biomedical Research; Birth Rate; COVID-19 pandemic; Cattle; Characteristics; Collection; Communication; Communities; Complex; Cryopreservation; Custom; Czech Republic; Data; Data Analyses; Development; Disasters; Disease; Disease model; Documentation; Domestic Animals; Dose; Embryo; Embryo Transfer; Ensure; Evaluation; Evaluation Indexes; Female; Ficoll; Follicle Stimulating Hormone; Foundations; Genetic; Genetic Variation; Genomics; Genotype; Goals; Gonadotropins; Grant; Hormones; Human Chorionic Gonadotropin; Hybrids; Implant; In Vitro; Inbred Strain; Inbred Strains Rats; Inbreeding; Infrastructure; Institution; Japan; Laboratories; Laboratory Research; Methods; Modeling; Monoclonal Antibody R24; Mus; PMSG (Gonadotropins); Parents; Phenotype; Positioning Attribute; Process; Propylene Glycols; Protocols documentation; Publications; Rat Genome Database; Rat Strains; Rattus; Recombinant Inbred Strain; Recombinants; Recovery; Reporting; Research; Research Personnel; Resources; Serum; Sucrose; Superovulation; Survival Rate; System; Toxic effect; Translations; animal resource; clinical translation; clinically relevant; embryo cryopreservation; embryo quality; ethylene glycol; forward genetics; genomic data; genomic locus; improved; in vivo; inhibin; natural Blastocyst Implantation; novel strategies; personalized approach; pregnant; preservation; programs; pup; repository; reproductive; restoration; success; tool; trait; transcriptomics; zygote

Project Summary The ongoing goal of this program is to extend the use of rat models for clinical translation through establishing an animal resource and genomic dataset to facilitate the translation of laboratory research to clinical relevance. Specifically, the Hybrid Rat Diversity Panel (HRDP) resource provides the research community with a foundation for systems genetics and a tool for forward genetics. The HRDP parent award aims to 1) to continue to establish the 96 strain Hybrid Rat Diversity Panel comprised of 33 classic inbred strains, 30 HXB/BXH recombinant inbred strains and 33 FXLE/LEXF recombinant inbred strains through embryo rederivation and importation, 2) to characterize, maintain and distribute the HRDP, 3) to provide genomic and transcriptomic sequencing of the HRDP and 4) and provide data analysis, integration and dissemination primarily through the Rat Genome Database (RGD). Specifically, this supplement will 1) evaluate embryo quality pre- and post-cryopreservation, 2) improve the efficiency of embryo collection for cryopreservation, and 3) develop cryopreservation protocols customized to strain characteristics. Together these results will have broad utility the scientific community through the genetic and phenotypic diversity represented in the strains selected for the supplement project in combination with the available data from the HRDP parent award. Data and protocols will be made available to the research community through the Rat Genome Database, publications, and through rat repositories.

项目概要 该计划的持续目标是扩大大鼠模型在临床转化中的使用 通过建立动物资源和基因组数据集来促进翻译 实验室研究与临床相关性。具体来说，杂交大鼠多样性小组 (HRDP) 资源为研究界提供了系统遗传学的基础和工具 正向遗传学。 HRDP家长奖旨在1）继续建立96品系 杂交大鼠多样性面板由 33 个经典近交系、30 个 HXB/BXH 重组体组成 通过胚胎再衍生和 33 个 FXLE/LEXF 重组近交系 导入，2) 表征、维护和分发 HRDP，3) 提供基因组和 HRDP 的转录组测序和 4) 并提供数据分析、整合和 主要通过大鼠基因组数据库（RGD）传播。具体而言，本补充 将 1) 评估冷冻保存前后的胚胎质量，2) 提高冷冻保存的效率 用于冷冻保存的胚胎收集，以及 3) 开发定制的冷冻保存方案 应变特性。这些结果将对科学界产生广泛的效用 通过所选菌株所代表的遗传和表型多样性 补充项目结合 HRDP 母奖的可用数据。数据 实验方案将通过大鼠基因组提供给研究界 数据库、出版物和大鼠存储库。