Hybrid Rat Diversity Program

项目摘要 这项计划的持续目标是扩大大鼠模型在临床翻译中的使用 通过建立动物资源和基因组数据库，促进动物资源的翻译 实验室研究与临床相关。具体地说，杂交大鼠多样性小组(HRDP) 资源为研究社区提供了系统遗传学的基础和工具 正向遗传学。为了实现这一目标，我们提出了以下四个具体目标：1) 继续建立由33个经典近交系组成的96品系杂交大鼠多样性小组 30个HXB/BXH重组自交系和33个FXLE/LEXF重组自交系 通过胚胎再衍生和进口，2)表征、维护和分配 HRDP，3)提供HRDP的基因组和转录序列，以及4)并提供 主要通过大鼠基因组数据库进行数据分析、整合和传播 (RGD)。这一资源将用于研究复杂疾病特征的机制，通过 通过向研究人员提供可再生的近交系大鼠面板来实现系统遗传学 菌株和生物库组织，可重复使用的表型数据，可重复使用的基因组和转录组 数据，以及通过HRDP探索、分析和可视化这些资源的基础设施 位于RGD的门户。实验大鼠和来自整个HRDP小组的生物库组织可以 用于其他基因组研究，包括表观基因组、蛋白质组和代谢组。《老鼠》 资源与研究中心(RRRC)正在与HRDP合作，为 研究界。人权与发展计划将通过以下方式对科学界产生广泛的效用 用于研究的96株菌株的遗传和表型多样性 高血压、中风、糖尿病、癌症、关节炎、生殖障碍、哮喘、衰老、酒精 偏爱、焦虑、运动和上瘾。

Project Summary The ongoing goal of this program is to extend the use of rat models for clinical translation through establishing an animal resource and genomic dataset to facilitate the translation of laboratory research to clinical relevance. Specifically, the Hybrid Rat Diversity Panel (HRDP) resource provides the research community with a foundation for systems genetics and a tool for forward genetics. To achieve this goal, we propose the following four specific aims: 1) to continue to establish the 96 strain Hybrid Rat Diversity Panel comprised of 33 classic inbred strains, 30 HXB/BXH recombinant inbred strains and 33 FXLE/LEXF recombinant inbred strains through embryo rederivation and importation, 2) to characterize, maintain and distribute the HRDP, 3) to provide genomic and transcriptomic sequencing of the HRDP and 4) and provide data analysis, integration and dissemination primarily through the Rat Genome Database (RGD). This resource will be used to study mechanisms of complex disease traits through systems genetics by providing investigators with access to a renewable panel of inbred rat strains and biobanked tissues, reusable phenotypic data, reusable genomic and transcriptomic data, and the infrastructure to explore, analyze and visualize these resources through the HRDP Portal at RGD. Experimental rats and biobanked tissues from the entire HRDP panel can be used for other omic studies, including the epigenome, proteome, and metabolome. The Rat Resource & Research Center (RRRC) is partnering with the HRDP to provide the rat models to the research community. The HRDP will have broad utility to the scientific community through the genetic and phenotypic diversity represented in the 96 strain panel used to study hypertension, stroke, diabetes, cancer, arthritis, reproductive disorders, asthma, aging, alcohol preference, anxiety, exercise and addiction.