Hybrid Rat Diversity Program

项目摘要 这个项目的持续目标是将大鼠模型用于临床转化 通过建立动物资源和基因组数据集， 实验室研究与临床相关性。杂交大鼠多样性小组（HRDP） 资源为研究社区提供了系统遗传学的基础和工具， 遗传学前沿为达致这个目标，我们提出以下四个具体目标： 继续建立由33个经典近交系组成的96系杂交大鼠多样性小组 HXB/BXH重组自交系30株，FXLE/LEXF重组自交系33株 通过胚胎再衍生和进口，2）表征，维持和分配 HRDP，3）提供HRDP的基因组和转录组测序，以及4）并提供 主要通过大鼠基因组数据库进行数据分析、整合和传播 （RGD）。该资源将用于研究复杂疾病特征的机制， 系统遗传学，为研究人员提供了一个可再生的小组近交系大鼠 菌株和生物库组织，可重复使用的表型数据，可重复使用的基因组和转录组 数据，以及通过HRDP探索、分析和可视化这些资源的基础设施 入口在RGD。实验大鼠和来自整个HRDP组的生物库组织可以 用于其他组学研究，包括表观基因组、蛋白质组和代谢组。大鼠 资源与研究中心（RRRC）与HRDP合作，为以下动物提供大鼠模型： 研究界。HRDP将通过以下方式对科学界产生广泛的效用： 遗传和表型多样性代表了96个菌株面板用于研究 高血压、中风、糖尿病、癌症、关节炎、生殖障碍、哮喘、衰老、酒精 偏好、焦虑、锻炼和成瘾。

Project Summary The ongoing goal of this program is to extend the use of rat models for clinical translation through establishing an animal resource and genomic dataset to facilitate the translation of laboratory research to clinical relevance. Specifically, the Hybrid Rat Diversity Panel (HRDP) resource provides the research community with a foundation for systems genetics and a tool for forward genetics. To achieve this goal, we propose the following four specific aims: 1) to continue to establish the 96 strain Hybrid Rat Diversity Panel comprised of 33 classic inbred strains, 30 HXB/BXH recombinant inbred strains and 33 FXLE/LEXF recombinant inbred strains through embryo rederivation and importation, 2) to characterize, maintain and distribute the HRDP, 3) to provide genomic and transcriptomic sequencing of the HRDP and 4) and provide data analysis, integration and dissemination primarily through the Rat Genome Database (RGD). This resource will be used to study mechanisms of complex disease traits through systems genetics by providing investigators with access to a renewable panel of inbred rat strains and biobanked tissues, reusable phenotypic data, reusable genomic and transcriptomic data, and the infrastructure to explore, analyze and visualize these resources through the HRDP Portal at RGD. Experimental rats and biobanked tissues from the entire HRDP panel can be used for other omic studies, including the epigenome, proteome, and metabolome. The Rat Resource & Research Center (RRRC) is partnering with the HRDP to provide the rat models to the research community. The HRDP will have broad utility to the scientific community through the genetic and phenotypic diversity represented in the 96 strain panel used to study hypertension, stroke, diabetes, cancer, arthritis, reproductive disorders, asthma, aging, alcohol preference, anxiety, exercise and addiction.