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New Targets for Reproductive Control of Mosquito Vectors

蚊媒生殖控制的新目标

基本信息

批准号：
10586390
负责人：
Laura C Harrington
金额：
$ 54.04万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-06-01 至 2027-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10586390
关键词：
Aedes Affect Ammonium Anopheles Genus Arbovirus Infections Attention Behavior Behavioral Biological Assay Biology Blood CRISPR/Cas technology Cell Nucleus Cell physiology Ceramics Charge Chikungunya virus Chromatography Complex Culicidae Data Dengue Dengue Virus Development Disease Vectors Drosophila genus Ecdysone Ecdysteroids Event Female Fertility Fractionation Funding Gene Expression Gene Proteins Genes Gland Goals Grant Homologous Gene Human Hydrophobicity Hydroxyapatites Individual Infection Insecta Insecticides Interruption Juvenile Hormones Life Longevity Maintenance Mass Spectrum Analysis Mediating Men&apos s Role Methods Molecular Mosquito Control Mosquito-borne infectious disease Movement Oviposition Partner in relationship Physiological Play Population Production Proteins Proteolysis RNA Interference Refractory Reproduction Reproductive Biology Research Rest Role Secretory Cell Seminal Seminal fluid Serine Protease Solid Source Testing Time Virus Work Yellow Fever ZIKA base chikungunya design egg experience feeding fitness inhibitor insight knock-down life history male male fertility mating behavior metabolomics mutant novel peptide analog peptide hormone reproductive reproductive fitness reproductive success reproductive tract response screening small molecule sperm cell sperm function sperm protein sperm viability success tool transcriptome sequencing transmission process vector vector control vector mosquito

项目摘要

New Targets for Reproductive Control of Mosquito Vectors Project summary/abstract In this project, we will investigate the actions of specific male seminal fluid molecules in promoting fertility and fitness of the major disease vector of dengue, Zika yellow fever and chikungunya viruses, Aedes aegypti. We will also define and analyze Aedes male and female molecules that associate with and support sperm. Despite the crippling impact of arbovirus infections, conventional mosquito control using insecticides can be operationally difficult and is often ineffective. Strategies to replace or reduce populations through modified male-releases show promise, but their success requires a deep understanding of mosquito reproductive biology. Ae. aegypti males transfer molecules in their seminal fluid to females during mating that induce female behavioral and physiological changes, including stimulation of egg development and oviposition, increased survival, and reluctance to re-mate with subsequent males. Despite the potential of these molecules as vector-control targets, the molecular identity and actions of these molecules are as yet unknown. During the current funding period, we utilized the latest tools to gain novel insights into key aspects of male mating success, sperm biology and female reproductive fitness, including identifying a limited subset of seminal fluid proteins (Sfps) that induce post-mating changes in females, and small-molecules such as juvenile hormone (JHIII) in seminal fluid. We propose two specific aims to (1) identify the role of individual male seminal molecules on female remating, fecundity, fertility, oviposition, lifetime reproductive success and survival and (2) to evaluate the role of male- and female-derived molecules on sperm storage, function, and survival. Our long-term goal is to design and deploy vector control strategies that target these key components of mosquito life history to reduce the tremendous global burden of mosquito-borne diseases.

蚊媒繁殖控制的新靶点项目概要/摘要在这个项目中，我们将研究特定的男性精液分子在生殖系统中的作用。促进登革热、寨卡黄热病等主要疾病媒介的繁殖力和适应性和基孔肯雅病毒、埃及伊蚊。我们还将定义和分析伊蚊男性和支持精子的女性分子。尽管这场灾难虫媒病毒感染的影响，使用杀虫剂的常规蚊子控制可以操作困难，而且往往效率低下。取代或减少人口的战略通过修改男性释放显示的承诺，但他们的成功需要一个深刻的了解蚊子的生殖生物学。AE.埃及雄性转移分子在交配过程中向雌性提供精液，生理变化，包括刺激卵发育和产卵，增加了存活率，并且不愿意与随后的雄性重新交配。尽管这些分子作为载体控制靶标的潜力、分子特性和作用这些分子中有多少是未知的。在当前融资期间，我们利用最新的工具，以获得新的见解，男性交配成功的关键方面，精子生物学和女性生殖健康，包括确定一个有限的子集，诱导雌性交配后变化的液体蛋白（Sfps）和小分子如精液中保幼激素（JHIII）。我们提出两个具体目标：（1）确定单个雄性精液分子对雌性再交配，生育力，生育力，产卵，终生繁殖成功率和存活率和（2）评估男性和女性衍生分子对精子储存、功能和存活的作用。我们的长期目标是设计和部署针对这些疾病的病媒控制战略，蚊子生活史的关键组成部分，以减少巨大的全球负担，蚊子传播的疾病