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Male accessory gland proteins of the dengue vector mosquito Aedes aegypti: potent

登革热媒介蚊子埃及伊蚊的雄性附属腺蛋白：有效

基本信息

批准号：
7652394
负责人：
Laura C Harrington
金额：
$ 19.25万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-07-10 至 2011-06-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): In this study we will use a novel combination of genomics tools with field studies to address the function of male reproductive proteins (Acps for Accessory gland proteins) of the dengue vector mosquito, Aedes aegypti, and their potential as targets for genetic control strategies. Male Acps were first identified in Drosophila melanogaster, where they have been shown to have dramatic effects on the reproductive biology of the female. Individual Acps identified from male Drosophila affect female egg production and egg laying, sperm storage, mating and feeding behaviors. Earlier studies with Ae. aegypti demonstrated an effect of "matrone", a partially purified male accessory gland substance, on female behavior and physiology. Matrone or accessory gland fluid injected into female Ae. aegypti has affected female sexual refractoriness, blood feeding behavior, blood digestion, flight, ovarian development, and oviposition. This early work and our own preliminary data indicate that male Acps will be important in Ae. aegypti and makes it imperative to investigate them in detail and determine their function. We will test the hypothesis that Ae. aegypti males transfer Acps to females during mating, and that these proteins have a significant impact on the female mosquito biology and reproductive fitness. Ultimately, these Acps may influence a variety of female traits that drive vector-borne disease dynamics. PUBLIC HEALTH RELEVANCE In this study we will identify male reproductive proteins of the dengue vector mosquito, Aedes aegypti. We will investigate the impact of these proteins on female vector biology and their potential as targets for genetic control strategies though a novel combination of laboratory and field studies.

描述（由申请人提供）：在这项研究中，我们将使用基因组学工具与实地研究的新型组合来解决登革热媒介蚊子埃及伊蚊的雄性生殖蛋白（Acps，即辅助腺蛋白）的功能，以及它们作为遗传控制策略目标的潜力。雄性 Acps 首次在黑腹果蝇中被发现，并被证明对雌性的生殖生物学具有显着影响。从雄性果蝇中鉴定出的个体 Acps 会影响雌性的产卵和产卵、精子储存、交配和摄食行为。早期对 Ae 的研究。埃及伊蚊证明了“matrone”（一种部分纯化的雄性附属腺物质）对雌性行为和生理的影响。将Matrone或附属腺液注入雌性Ae体内。埃及伊蚊影响女性性难耐、吸血行为、血液消化、飞行、卵巢发育和产卵。这项早期工作和我们自己的初步数据表明，雄性 Acps 在 Ae 中很重要。埃及伊蚊，因此有必要对其进行详细研究并确定其功能。我们将检验 Ae 的假设。雄性埃及伊蚊在交配过程中将 Acps 转移给雌性，这些蛋白质对雌性蚊子的生物学和生殖健康具有重大影响。最终，这些 Acps 可能会影响多种女性特征，从而驱动媒介传播疾病的动态。公共卫生相关性在这项研究中，我们将鉴定登革热媒介蚊子埃及伊蚊的雄性生殖蛋白。我们将通过实验室和现场研究的新颖结合来研究这些蛋白质对雌性媒介生物学的影响及其作为遗传控制策略目标的潜力。