权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

New Targets for Reproductive Control of Mosquito Vectors

蚊媒生殖控制的新目标

基本信息

批准号：
10084795
负责人：
Laura C Harrington
金额：
$ 44.72万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-06-01 至 2022-09-20
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10084795
关键词：
Address Aedes Behavior Biological Assay Blood Chikungunya virus Child Clustered Regularly Interspaced Short Palindromic Repeats Colombia Culicidae DNA Sequence DNA sequencing Dengue Dengue Virus Digestion Disease Disease Vectors Exhibits Feeding behaviors Female Fertility Florida Fractionation Funding Future Gene Expression Genes Genetic Variation Gland Goals High-Throughput RNA Sequencing Incidence Injections Insecta Knock-out Male Sterility Mass Spectrum Analysis Measures Mediating Men&apos s Role Methods Molecular Mosquito Control Mutate Mutation Ovulation Partner in relationship Phenotype Physiological Physiology Population Process Production Proteins Refractory Reproduction Reproductive Physiology Scourge Seminal Seminal fluid Source Testing Thailand Variant Virus Work Yellow Fever Yellow fever virus ZIKA Zika Virus base chikungunya design disease transmission disorder control effectiveness evaluation egg emerging pathogen feeding genome editing human disease in vivo innovation knock-down male mating behavior mosquito reproduction novel pathogen reproductive reproductive success response tool trait transcriptome transmission process vector vector control vector mosquito vector-borne infection viral transmission

项目摘要

New Targets for Reproductive Control of Mosquito Vectors Project summary/abstract In this project, we will investigate the male seminal fluid proteins (SFPs) of a major disease vector of dengue, Zika and chikungunya viruses: the yellow fever mosquito (Aedes aegypti). Insect SFPs trigger major changes in female physiology, conducive to high fertility (e.g. increased egg production), regulating re-mating and, in mosquitoes, potentially regulating blood feeding physiology. Dengue is an old scourge, but new pathogens such as chikungunya and Zika that cycled silently in their sylvatic backdrop have surged forward and crossed hemispheres with a vengeance. There is an immediate need to develop novel and effective vector control strategies for these vectors. In the present funding period, we identified Aedes SFPs and obtained bioactive fractions and identified components. We developed assays for testing the effects of the components, and have been successful in using CRISPR-based genome editing to mutate our first candidates for bioactive SFPs. We also determined the effect of mating on SFP stores (and production) in males, and on the transcriptome of the mated female. In this renewal application, for continued support for this project, we propose three specific aims to (1) identify and functionally characterize bioactive SFPs in vivo in Ae. aegypti, (2) determine female molecular responses to SFPs in general and physiological and molecular responses to specific, bioactive, SFPs and (3) understand the phenotypic and genetic variation of SFPs and their responsive targets in field relevant populations of this mosquito vector. Our goal is to identify SFPs, and SFP targets, that could be manipulated as a novel means of reproductive control, or to modify the blood feeding physiology of these insects that transmit deadly pathogens, with the long-term goal to reduce or eliminate virus transmission to people.

蚊媒生殖控制的新靶点