GENETIC MANAGEMENT OF MACAQUES BY DNA RFLP ANALYSIS

通过 DNA RFLP 分析对猕猴进行遗传管理

• 批准号: 3566196
• 负责人: DAVID Glenn SMITH
• 金额: $ 7.92万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-30 至 1993-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3566196
• 关键词: AIDS; DNA; Macaca mulatta; animal colony; animal population genetics; endonuclease; gene frequency; genetic markers; germ free condition; nucleic acid structure; reproduction; veterinary science

The development of Retrovirus and Herpes B virus Specific Pathogen Free (SPF), rhesus macaques has become a primary goal in captive breeding programs. These SPF colonies are important for the conduct of AIDS research and in protecting the health of personnel who care for animals or handle animal tissue. The goal of this project is to develop technology that will permit the monitoring of genetic diversity in the SPF rhesus macaque breeding colonies. Rhesus macaque nuclear DNA will be examined with restriction enzymes and clones to identify hypervariable restriction fragment length polymorphisms, (RFLP). Three large group housed populations of rhesus macaques with known paternity data will then be examined with RFLP analysis to determine the efficiency of hypervariable regions in defining pedigrees. At the same time protocols for use of different kinds of tissue samples such as unrefrigerated whole blood, saliva, hair, and skin will also be developed to extend the research applications of this technology. Once the technique is refined pedigree determination by RFLP analysis will be applied to SPF colony founder stock at the California Primate Research Center and offered as a service to other institutions to extend beyond the duration of this grant. A long term research goal of this project is to compare RFLP regions with medical, reproductive, mortality, and morbidity data on individuals to hopefully identify genetic markers for disease predisposition and/or reproductive performance.

逆转录病毒和B型疱疹病毒特异性研究进展 无病原体（SPF），恒河猴已成为主要目标 在圈养繁殖计划中。 这些SPF菌落很重要 开展艾滋病研究和保护 照料动物或处理动物组织的人员。 目标 该项目的目的是开发技术， SPF恒河猴遗传多样性监测 繁殖殖民地 恒河猴的核DNA 用限制性内切酶和克隆进行检测， 高变限制性片段长度多态性（RFLP）。 三个大的群体居住的恒河猴种群， 然后将用RFLP分析检查已知的亲子关系数据， 确定高变区在定义 血统 与此同时，不同类型的使用协议 组织样本，如未冷藏的全血，唾液，毛发， 还将开发皮肤以扩展研究应用 of this technology技术. 一旦技术完善血统 将通过RFLP分析的测定应用于SPF菌落 加州灵长类动物研究中心的创始人股票， 作为一项服务提供给其他机构，以扩大 这一补助金的期限。 本项目的长期研究目标 是将RFLP区域与医疗、生殖、 死亡率和发病率数据，希望能确定 疾病易感性和/或生殖遗传标记 性能