Cellular Visualization and Analysis

细胞可视化和分析

• 批准号: 7684762
• 负责人: Thomas E Finger
• 金额: $ 33.89万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7684762
• 关键词: 3-Dimensional; Advanced Development; Biological; Biomedical Research; Brain; Chemicals; Collaborations; Complex; Computer Graphics; Confocal Microscopy; Consultations; Costs and Benefits; Coupled; Data; Data Set; Development; Discipline; Electron Microscopy; Electrons; Epithelium; Functional Imaging; Goals; Histological Techniques; Image; Imagery; In Situ Hybridization; Individual; Label; Laboratories; Maps; Methodology; Methods; Microscopic; Molecular Probes; Neurosciences; Other Resources; Procedures; Process; Protocols documentation; Purpose; Quality Control; Reagent; Research; Research Personnel; Services; Statistical Methods; Structure; System; Taste Buds; Techniques; Technology; Test Result; Testing; Tissues; Universities; cost; experience; immunocytochemistry; innovation; insight; instrument; instrumentation; light microscopy; multi-photon; olfactory bulb; programs; ranpirnase; relating to nervous system; software development

A major focus of research in the RMTSC laboratories is the use of specific molecular probes to delineate cellular composition and function in chemosensory systems. The major function of this core is to facilitate research of the participating investigators which relies on anatomically oriented methods to probe the organization, development or function of chemosensory epithelia or neural centers primarily involved in processing chemosensory information. The Core functions are encompassed by 3 specific Aims: 1. Support & Development of advanced histoiogical techniques 2. Confocal and Electron Microscopy 3. Quantitative Analysis and Visualization of Complex Anatomical Data Sets. Although each of the RMTSC laboratories utilizes anatomical techniques as a part of their overall research program, not all laboratories have equal access or experience in using advanced methodologies such as dual-labeling, functional imaging and electron microscopy. This Core facilitates sharing of expertise among the laboratories and makes available to all, specialized facilities available only at one of the participating Universities. Not only will the Core serve the local RMTSC investigators, but it will also serve as a resource for other chemosensory researchers especially in regards to development of software for mapping chemosensory representation in the brain.

RMTSC实验室的一个主要研究重点是使用特定的分子探针来描绘化学感受系统中的细胞组成和功能。该核心的主要功能是促进参与研究者的研究，其依赖于解剖学导向的方法来探测主要参与处理化学感觉信息的化学感觉上皮或神经中心的组织、发育或功能。核心职能包含三个具体目标：1.支持和发展先进的组织学技术2.共聚焦显微镜和电子显微镜3.复杂解剖数据集的定量分析与可视化。虽然每个RMTSC实验室利用解剖技术作为其整体研究计划的一部分，但并非所有实验室都有使用先进方法（如双标记，功能成像和电子显微镜）的平等机会或经验。这一核心促进了实验室之间的专业知识共享，并向所有人提供只有一所参与大学才能提供的专门设施。核心不仅将为当地的RMTSC调查人员服务，而且还将作为一种资源 对于其他化学感受研究人员，特别是在开发用于绘制大脑中化学感受表征的软件方面。