Nitric Oxide-Releasing Glucose Biosensors

摘要 这项建议的目的是探索利用新型NO释放传感器膜来开发 改进的皮下葡萄糖生物传感器，在体内发挥更可靠的作用。使用本地NO的概念 释放以增强体内皮下传感器的性能代表了一种新的方法，可以 克服阻碍开发可靠工作的体内生物传感器的关键障碍 一旦植入患者体内。目前关于NO在血管生成中的作用的科学知识， 吞噬、血栓形成和伤口愈合提示NO在传感器界面的受控释放 可以增加流向传感器的血流量，减少炎症，并通过抑制细菌促进伤口愈合 粘附性和最小化后续胶囊的厚度。因此，良好的生物兼容性可能会使 宿主的生理反应，目前的皮下生物传感器的体内性能将是 有了显著改善。在合成具有足够分析物的NO释放传感器膜之后 渗透性和广泛的NO释放特性，包括通量和持续时间，我们将：1)评估 这种材料体内组织生物相容性随NO释放特性的变化；2)制备功能性 NO释放型葡萄糖微型传感器；3)评价此类传感器在猪体内的分析性能 模特。 这项拟议的研究有可能导致植入式葡萄糖传感器的生物污垢减少。 以及细菌感染、促进伤口愈合和改进分析性能。一种功能性葡萄糖 具有这些特征的传感器将影响数百万糖尿病患者，他们可能是 连续血糖监测设备。项目叙事 这项建议的目的是探索使用新型的一氧化氮(NO)释放传感器膜来 开发改进的皮下葡萄糖生物传感器，在体内发挥更可靠的作用。建议数 研究有可能导致植入式葡萄糖传感器显示减少生物污垢和细菌 感染、促进伤口愈合和改进分析性能。

ABSTRACT The objective of this proposal is to explore the use of novel NO releasing sensor membranes for developing improved subcutaneous glucose biosensors that function more reliably in vivo. The concept of using local NO release to enhance the performance of in vivo subcutaneous sensors represents a novel approach that may overcome the key obstacles that have prevented the development of in vivo biosensors that function reliably once implanted in patients. Current scientific knowledge regarding the role of NO in angiogenesis, phagocytosis, thrombosis, and wound healing suggests that the controlled release of NO at a sensor interface may increase blood flow to the sensor, reduce inflammation, and promote wound healing by inhibiting bacterial adhesion and minimizing the thickness of the ensuing capsule. Favorable biocompatibility thus may minimize host physiological responses such that the in vivo performance of current subcutaneous biosensors would be dramatically improved. Following the synthesis of NO-releasing sensor membranes with adequate analyte permeability and a wide range of NO release characteristics including flux and duration, we will: 1) evaluate the tissue biocompatibility of such materials in vivo as a function of NO release properties; 2) fabricate functional NO-releasing glucose microsensors; 3) evaluate the in vivo analytical performance of such sensors in a pig model. The proposed research has the potential to lead to implantable glucose sensors that exhibit reduced biofouling and bacterial infection, enhanced wound healing, and improved analytical performance. A functional glucose sensor with these characteristics would impact millions of diabetic patients who are potential candidates for continuous glucose monitoring devices. PROJECT NARRATIVE The objective of this proposal is to explore the use of novel nitric oxide (NO)-releasing sensor membranes for developing improved subcutaneous glucose biosensors that function more reliably in vivo. The proposed research has the potential to lead to implantable glucose sensors that exhibit reduced biofouling and bacterial infection, enhanced wound healing, and improved analytical performance.