Nitric Oxide-Releasing Glucose Biosensors

释放一氧化氮的葡萄糖生物传感器

• 批准号: 6663153
• 负责人: Mark H Schoenfisch
• 金额: $ 33.63万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-25 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6663153
• 关键词: adhesions; angiogenesis; antiinfective agents; bacteria; bioengineering /biomedical engineering; biomaterial compatibility; biomedical equipment development; biosensor device; blood glucose; capillary; histology; implant; laboratory rat; medical implant science; nitric oxide; slow release drug; wound healing

DESCRIPTION (provided by applicant): The objective of the proposed research is to develop a new class of nitric oxide (NO)-releasing bioanalytical sensors and evaluate their utility for in vivo subcutaneous sensor applications. The concept of using local NO release to enhance the performance of in vivo subcutaneous sensors represents a novel approach to overcoming the difficulties that have thus far prevented the development of reliable in vivo biosensors. Current scientific knowledge regarding the role of NO in angiogenesis, phagocytosis, thrombosis, and wound healing suggests that controlled in situ NO release may effectively help reduce biofouling and increase blood flow to the sensor, thus minimizing physiological responses that tend to diminish the in vivo performance of subcutaneous sensors. Specifically, it is envisioned that slow release of NO locally at the implant site will both a) reduce bacterial adhesion and associated biofouling problems, and b) enhance overall wound healing and the formation of capillaries near the implant site such that analyte diffusion from blood to the sensor electrode is enhanced. The fundamental question to be answered by the proposed research is whether electrochemical bioanalytical sensors that continuously release NO can be prepared with improved biocompatibility without compromising the sensor's analytical response. Thus, we seek to determine if the chemistries required for sustained NO release can be made compatible with the chemistries required for selective and sensitive detection of glucose. In addition, we aim to employ micropatterning methods to create unique sensor architectures that support NO release while retaining superior analytical sensitivity. The versatility of the sol-gel process in terms of tuning NO release properties by varying the amount of aminosilane, combined with the variety of pattern geometries that may be created using micropatterning techniques, will allow for the development of numerous types of heterogeneous NO-releasing surfaces with a broad range of possible applications.

描述（由申请人提供）：拟议研究的目的是开发新的一氧化氮（NO）释放生物分析传感器，并评估其用于体内皮下传感器应用的实用性。使用局部NO释放来增强体内皮下传感器的性能的概念是一种新的方法来克服迄今为止无法开发可靠的体内生物传感器的困难。当前关于NO在血管生成，吞噬作用，血栓形成和伤口愈合中的作用的科学知识表明，原位控制NO释放可能有效地减少生物污染物并增加流向传感器的血液流动，从而最大程度地减少皮下传感器体内的生理反应。具体而言，可以预见的是，在植入物部位不局部的NO局部缓慢释放将同时a）减少细菌粘附和相关的生物污染问题，b）增强了整体伤口愈合，并在植入物部位附近的毛细血管形成，从而增强了分析物从血液从血液到传感器电极的扩散。拟议的研究要回答的基本问题是，如果不损害传感器的分析响应，是否可以改善生物相容性，是否可以改善生物相容性。因此，我们试图确定是否可以持续的化学分子与选择性和敏感葡萄糖所需的化学分配兼容。此外，我们旨在采用微图解方法来创建独特的传感器体系结构，在保留出色的分析灵敏度的同时，支持无释放。 Sol-Gel工艺在调整无释放性能方面的多功能性通过改变氨基硅烷的量，并结合使用微图解技术创建的各种模式几何形状，将允许开发多种类型的异构无频率的表面以及可能应用的广泛应用。