Nitric Oxide-Releasing Glucose Biosensors

描述(申请人提供)：拟议研究的目标是开发一类新的释放一氧化氮(NO)的生物分析传感器，并评估其在体内皮下传感器应用的有效性。使用局部NO释放来提高体内皮下传感器的性能的概念代表了一种新的方法，以克服迄今为止阻碍可靠的体内生物传感器发展的困难。目前关于NO在血管生成、吞噬、血栓形成和伤口愈合中的作用的科学知识表明，原位受控的NO释放可能有效地帮助减少生物污垢和增加流向传感器的血流量，从而最小化往往会降低皮下传感器体内性能的生理反应。具体地说，可以预见，NO在植入部位局部的缓慢释放将a)减少细菌黏附和相关的生物污垢问题，以及b)促进整体伤口愈合和植入部位附近毛细血管的形成，从而增强分析物从血液到传感器电极的扩散。这项拟议的研究要回答的基本问题是，能否在不影响传感器分析响应的情况下，制备出持续释放NO的电化学生物分析传感器，并改善其生物兼容性。因此，我们试图确定持续释放NO所需的化学物质是否可以与选择性和灵敏地检测葡萄糖所需的化学物质相兼容。此外，我们的目标是使用微图案化方法来创建独特的传感器架构，该架构支持无释放，同时保持卓越的分析灵敏度。在通过改变氨基硅烷的量来调节NO释放特性方面，溶胶凝胶法的多功能性，与可使用微图案化技术产生的各种图案几何形状相结合，将允许开发具有广泛可能应用的多种类型的异质NO释放表面。

DESCRIPTION (provided by applicant): The objective of the proposed research is to develop a new class of nitric oxide (NO)-releasing bioanalytical sensors and evaluate their utility for in vivo subcutaneous sensor applications. The concept of using local NO release to enhance the performance of in vivo subcutaneous sensors represents a novel approach to overcoming the difficulties that have thus far prevented the development of reliable in vivo biosensors. Current scientific knowledge regarding the role of NO in angiogenesis, phagocytosis, thrombosis, and wound healing suggests that controlled in situ NO release may effectively help reduce biofouling and increase blood flow to the sensor, thus minimizing physiological responses that tend to diminish the in vivo performance of subcutaneous sensors. Specifically, it is envisioned that slow release of NO locally at the implant site will both a) reduce bacterial adhesion and associated biofouling problems, and b) enhance overall wound healing and the formation of capillaries near the implant site such that analyte diffusion from blood to the sensor electrode is enhanced. The fundamental question to be answered by the proposed research is whether electrochemical bioanalytical sensors that continuously release NO can be prepared with improved biocompatibility without compromising the sensor's analytical response. Thus, we seek to determine if the chemistries required for sustained NO release can be made compatible with the chemistries required for selective and sensitive detection of glucose. In addition, we aim to employ micropatterning methods to create unique sensor architectures that support NO release while retaining superior analytical sensitivity. The versatility of the sol-gel process in terms of tuning NO release properties by varying the amount of aminosilane, combined with the variety of pattern geometries that may be created using micropatterning techniques, will allow for the development of numerous types of heterogeneous NO-releasing surfaces with a broad range of possible applications.