Purchase of a 700MHz NMR Spectrometer for Liquid Applications

购买用于液体应用的 700MHz NMR 波谱仪

• 批准号: 7498283
• 负责人: JOHN MONTGOMERY
• 金额: $ 132.47万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-14 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7498283
• 关键词: Address; Age; Area; Arts; Biological Factors; Biological Process; Carbohydrates; Cell Nucleus; Chemicals; Chemistry; Complex; Cyclopentane; Detection; Development; Disease Progression; Electronics; Enzymes; Free Radicals; Funding; Health; Human; Institution; Investigation; Kinetics; Liquid substance; Michigan; Molecular; Molecular Conformation; NMR Spectroscopy; Nuclear Magnetic Resonance; Pharmaceutical Preparations; Prevention; Pyrrolidines; Relative (related person); Research Personnel; Research Project Grants; Resolution; Solutions; Structure; Students; System; Techniques; Technology; Time; Training; Transcription Coactivator; Universities; design; improved; instrument; instrumentation; public health relevance; pyrrolidine; small molecule; stem; stereochemistry; tetrahydrofuran; tool

DESCRIPTION (provided by applicant): Purchase of a 700MHz NMR Spectrometer for Liquid Applications Project Summary Solution nuclear magnetic resonance (NMR) spectroscopy is one of the most powerful spectroscopic tools available to the synthetic chemist for the elucidation of the structure of a molecule. Advances in computing power, high-field magnet technology, and improved electronics have dramatically increased both sensitivity and chemical shift dispersion such that detection of low natural abundance nuclei is now routine. Presently in the Chemistry department at the University of Michigan (UM, Ann Arbor) there is a rapidly growing gap between the existing capabilities offered by our aging/obsolete NMR spectrometers and the demands of our NIH-funded investigators. This demand stems from the fact that not only have the molecular systems under investigation grown in size and complexity, the questions being addressed by NMR are also more ambitious and include atomic characterization of molecular configuration, conformation, dynamics, interactions and mechanisms. The availability of a high field, multi-channel NMR spectrometer dedicated to liquid applications will have a significant impact on the investigation of complex natural products bearing multiple stereocenters, determination of relative stereochemistry of 5-membered carbocyclic ring systems such as cyclopentanes, tetrahydrofurans, and pyrrolidines possessing up to 4 stereocenters, characterization of unstable organoboron and organosilicon intermediates, and time-resolved kinetic studies of reactive organometallic intermediates formed in low concentrations. High-resolution structural information will be highly valuable in designing small molecule isoxazolidine transcriptional activators, as well as probing how enzymes use free radicals to catalyze chemically difficult transformations. Progress in these research projects and the training of chemistry students in cutting-edge NMR techniques are hampered due to the lack of an accessible modern high field NMR spectrometer. Therefore, to close this gap, we propose the purchase of a multi-channel 700 MHz NMR spectrometer outfitted with the latest in cryogenically cooled, triple resonance probe technology. Additionally, access to such a state-of-the-art instrument will be made available to NIH-funded chemistry projects at nearby institutions such as the University of Toledo, where significant benefit would be realized in the area of complex carbohydrate synthesis. PUBLIC HEALTH RELEVANCE The NIH-funded projects described herein will contribute to a greater understanding of areas that impact human health. Advances enabled by the requested instrumentation include the discovery of potential drug leads, the understanding of biological processes involved in disease progression or prevention, and the development of new strategies in synthesis that enable creative solutions to research projects of this type.

描述（由申请人提供）：购买用于液体应用的700 MHz NMR光谱仪项目概要解决方案核磁共振（NMR）光谱是合成化学家用于阐明分子结构的最强大的光谱工具之一。计算能力、高场磁体技术和改进的电子技术的进步显着提高了灵敏度和化学位移分散度，使得低自然丰度核的检测现在已成为常规。目前，在密歇根大学（UM，安阿伯）的化学系，我们老化/过时的NMR光谱仪提供的现有功能与我们NIH资助的研究人员的需求之间的差距正在迅速扩大。这一需求源于这样一个事实，即不仅所研究的分子系统的大小和复杂性增加，NMR所解决的问题也更加雄心勃勃，包括分子构型、构象、动力学、相互作用和机制的原子表征。专用于液体应用的高场、多通道NMR光谱仪的可用性将对具有多个立体中心的复杂天然产物的研究、具有多达4个立体中心的5元碳环系统（如环戊烷、四氢呋喃和吡咯烷）的相对立体化学的测定、不稳定的有机硼和有机硅中间体的表征、和低浓度下形成的反应性有机金属中间体的时间分辨动力学研究。高分辨率的结构信息将是非常有价值的设计小分子异恶唑烷转录激活剂，以及探测酶如何使用自由基催化化学困难的转换。这些研究项目的进展和化学专业学生在尖端核磁共振技术方面的培训由于缺乏现代高场核磁共振光谱仪而受到阻碍。因此，为了缩小这一差距，我们建议购买一台配备最新低温冷却三重共振探针技术的多通道700 MHz NMR光谱仪。此外，获得这样一个国家的最先进的仪器将提供给国家卫生研究院资助的化学项目在附近的机构，如托莱多大学，其中显着的好处将在复杂的碳水化合物合成领域实现。公共卫生相关性本文描述的NIH资助的项目将有助于更好地了解影响人类健康的领域。所要求的仪器实现的进展包括发现潜在的药物线索，了解疾病进展或预防所涉及的生物过程，以及开发新的合成策略，从而为此类研究项目提供创造性的解决方案。