The automated measurement of foci in fluorecence microscopy

我们与丹麦技术大学 Flemming Hansen 博士的实验室合作，在图像分析程序 Image Pro Plus 中以宏的形式开发了全自动细胞识别和荧光焦点测量程序。这在检测大肠杆菌细胞中质粒和特定染色体位点形成的荧光灶的基本任务中非常成功。随着我们对荧光图像分析的需求变得越来越严格，我们不断开发和调整该系统来满足这些需求。我们正在开发一种分析工具 Inspect，它有助于分析具有两种颜色荧光信息的图像。结合数据收集程序，我们正在开发一系列电子表格程序来分析和描绘数据。

In collaboration with the laboratory of Dr, Flemming Hansen, Technical University of Denmark, we have developed a fully automated cell recognition and fluorescent focus-measuring program in the form of a macro within the image analysis program Image Pro Plus. This has been very successful in the basic tasks of detecting fluorescent foci formed by plasmids and specific chromosomal loci in cells of the bacterium Escherichia coli. As our needs for analysis of fluorescent images become more demanding, we continue to develop and adapt this system to meet them. We are developing an analytical tool Inspect that facilitates the analysis of images that have two color fluorescence information. In conjunction with the data collection programs, we are developing an array of spreadsheet programs to analyze and portray the data.