Role of Fucosyl Saccharides and O-GlcNAc Glycosylation in Neuronal Communication

岩藻糖基糖和 O-GlcNAc 糖基化在神经元通讯中的作用

• 批准号: 7846392
• 负责人: Linda C Hsieh-Wilson
• 金额: $ 48.29万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7846392
• 关键词: Acetylglucosamine; Address; Age; Alzheimer' s Disease; Animal Model; Animals; Applications Grants; Arts; Behavior; Behavioral Paradigm; Biochemistry; Brain; Brain Injuries; CREB1 gene; Carbohydrates; Cells; Chemicals; Chemistry; Cognition; Cognitive deficits; Communication; Communications Media; Complement; Complex; DNA; Defect; Dendrites; Dendritic Spines; Detection; Development; Disease; Drosophila melanogaster; Drug Addiction; Electrophysiology (science); Enzymes; Epilepsy; Exhibits; Fluorescence Microscopy; Fragile X Syndrome; Fucose; Funding; Galactose; Gel; Gene Expression; Genetic; Genetically Engineered Mouse; Glycoproteins; Goals; Grant; Human; Image; Imagery; Infection; Inflammation; Information Storage; Ischemia; Label; Laboratories; Lead; Learning; Lectin; Life; Link; Long-Term Potentiation; Maps; Measures; Mediating; Memory; Memory impairment; Mental Retardation; Methodology; Methods; Modeling; Modification; Molecular; Molecular Target; Monitor; Morphology; Mus; Nature; Neoplasm Metastasis; Nerve; Neurobiology; Neurodegenerative Disorders; Neurons; O-GlcNAc transferase; Oximes; Pathway interactions; Pharmacologic Substance; Phosphorylation; Physiological; Physiology; Play; Post-Translational Protein Processing; Probability; Process; Property; Protein Biosynthesis; Proteins; Proteome; Proteomics; Recovery; Regulation; Rodent; Role; Science; Signal Pathway; Signal Transduction; Site; Slice; Structure; Structure-Activity Relationship; Synapses; Synapsin I; Synapsins; Synaptic Transmission; Synaptic Vesicles; Synaptic plasticity; System; Time; United States National Institutes of Health; Up-Regulation; Work; aging brain; analog; base; carbohydrate structure; cognitive function; conditioned fear; cycloaddition; design; embryonic stem cell; glycosylation; human FRAP1 protein; improved; in vivo; insight; long term memory; morris water maze; neuron development; neuronal cell body; neuronal growth; neurotransmitter release; novel; novel therapeutics; pluripotency; postsynaptic; prepulse inhibition; protein structure function; public health relevance; relating to nervous system; response; self-renewal; small molecule; sugar; tool; trafficking; transcription factor

DESCRIPTION (provided by applicant): This revision application to 5R01 GM084724-06 has been submitted in response to Notice Number NOT-OD-09-058, entitled "NIH Announces the Availability of Recovery Act Funds for Competitive Revision Applications." The application represents a significant expansion of the scope of the original project to investigate the role of O-GlcNAc (O-linked N-acetyglucosamine) glycosylation in learning and memory. Developing an understanding of the molecular mechanisms that underlie learning and memory stands as one of the central challenges of modern science. Our proposed studies will focus on a carbohydrate modification that plays a central role in this process: O-GlcNAc glycosylation. O-GlcNAc glycosylation is a dynamic, intracellular modification found on proteins involved in gene expression, cell signaling, and synaptic plasticity. A major goal of our work is to develop an understanding of the molecular mechanisms by which this sugar influences neuronal communication and information storage. Long-term memory is widely believed to occur through changes in synapse number and strength during learning. Such changes, termed "synaptic remodeling," require new protein synthesis in dendrites, the branched projections on the cell that conduct nerve impulses from the synapse to the cell body. Blockade of protein synthesis has been shown to inhibit learning and memory, demonstrating a direct functional link between dendritic protein synthesis, synaptic remodeling, and behavior. Recently, we made the exciting discovery that O-GlcNAc glycosylation is required for activity-dependent protein synthesis in dendrites. Here, we will investigate this discovery in greater mechanistic detail to understand how O-GlcNAc glycosylation regulates dendritic protein synthesis and its consequences for synaptic plasticity. In addition, we will probe whether elimination of O-GlcNAc glycosylation in the brain leads to learning and memory deficits in mice. A unique feature of this proposal is the seamless integration of chemistry with challenging neurobiological studies. We believe that the combination of cutting-edge chemical tools with state-of- the-art neurobiological approaches will be necessary to address the complex, fundamental question of how memories are stored. The proposed studies will significantly advance our understanding of the structure-activity relationships of carbohydrates in the brain and reveal new insights into the molecular basis of learning and memory. At the same time, our studies may ultimately impact the development of pharmaceuticals by revealing novel molecular targets and processes for the treatment of cognitive deficits associated with aging, brain injury, mental retardation, and neurodegenerative disease. PUBLIC HEALTH RELEVANCE: A major goal of this work is to elucidate molecular mechanisms that underlie neuronal communication and hence form the basis of learning and memory. Through the discovery of novel small molecules, proteins and pathways involved in neural communication and function, this work may aid ultimately in the development of new pharmaceuticals designed to improve cognition deficits associated with aging and neurodegenerative disease.

描述（由申请人提供）：已提交给5R01 GM01G084724-06的修订申请，以响应通知号而不是OD-09-09-058，标题为“ NIH宣布竞争性修订申请的恢复法案资金的可用性”。该应用程序代表了原始项目范围的显着扩展，以研究O-GlCNAC（O-联轴N-环葡聚糖）在学习和记忆中的作用。 对学习和记忆为基础的分子机制发展成为现代科学的主要挑战之一。我们提出的研究将集中于在此过程中起着核心作用的碳水化合物修饰：O-GLCNAC糖基化。 O-GLCNAC糖基化是一种在涉及基因表达，细胞信号传导和突触可塑性的蛋白质上的动态，细胞内修饰。我们工作的主要目的是对这种糖影响神经元通信和信息存储的分子机制发展理解。人们普遍认为，长期记忆是通过学习过程中的突触数量和力量的变化而发生的。这种变化称为“突触重塑”，需要在树突中进行新的蛋白质合成，这是细胞上的分支投影，从而导致神经冲动从突触到细胞体。蛋白质合成的阻断已显示可抑制学习和记忆，证明了树突状蛋白质合成，突触重塑和行为之间的直接功能联系。最近，我们提出了令人兴奋的发现，即树突中活性依赖性蛋白质合成需要O-GlcNAC糖基化。在这里，我们将以更大的机械细节进行研究，以了解O-GlCNAC糖基化如何调节树突蛋白的合成及其对突触可塑性的影响。此外，我们将探测消除大脑中O-GLCNAC糖基化是否会导致小鼠的学习和记忆缺陷。 该提案的一个独特特征是化学与充满挑战的神经生物学研究的无缝整合。我们认为，必须将尖端的化学工具与最先进的神经生物学方法结合在一起，以解决如何存储记忆的复杂，基本问题。拟议的研究将大大提高我们对大脑碳水化合物的结构活性关系的理解，并揭示对学习和记忆的分子基础的新见解。同时，我们的研究最终可能会通过揭示新的分子靶标和过程来治疗与衰老，脑损伤，智力低下和神经退行性疾病相关的认知缺陷来影响药物的发展。 公共卫生相关性：这项工作的主要目标是阐明基于神经元沟通的分子机制，从而构成了学习和记忆的基础。通过发现新型的小分子，涉及神经交流和功能的蛋白质和途径，这项工作最终可能有助于开发新的药物，旨在改善与衰老和神经退行性疾病相关的认知缺陷。