MECHANISM OF HOST INVASION BY SCHISTOSOMES

血吸虫宿主入侵机制

• 批准号: 8170519
• 负责人: James H. McKerrow
• 金额: $ 0.71万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170519
• 关键词: Amino Acid Sequence; Biochemical; Bioinformatics; Blood Circulation; Chemicals; Computer Graphics; Computer Retrieval of Information on Scientific Projects Database; Data; Digestion; Extracellular Matrix; Funding; Genomics; Grant; Hemoglobin; Infection; Institution; Laboratories; Larva; Lipids; Maps; Methods; Molecular; Peptide Hydrolases; Peptide Sequence Determination; Plasma Proteins; Prevention approach; Protein Analysis; Proteins; Proteomics; Research; Research Personnel; Resources; Schistosoma; Schistosomiasis; Sequence Analysis; Source; Structural Models; Techniques; United States National Institutes of Health; interest; response; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Characterization of the molecular mechanisms of host invasion by schistosomes will be an important step in developing rational approaches to prevention or control of schistosomiasis. Our interest is in studying the biochemical and molecular mechanisms by which a proteolytic mixture, elaborated by schistosome larvae in response to lipid, facilitates degradation of host extracellular matrix and entrance of infectious larvae into the host bloodstream. This project combines chemical proteomics techniques with the wealth of recently available schistosome genomic sequence data to examine the key protein components that facilitate host invasion. Our methods include protein separation techniques followed mass spectrometric analysis of the proteins. We then analyze the derived protein sequences using bioinformatics and sequence analysis tools available through the Computer Graphics Laboratory. A related project is in studying schistosome proteolytic enzymes used for digestion of the host hemoglobin and other plasma proteins and for propagation of infection. Specifically, we are interested in mapping out the biochemical activation cascade for the schistosome digestive proteases. For these projects, we use CGL resources for structural modeling and sequence analysis of the proteolytic enzymes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 血吸虫入侵宿主的分子机制的表征将是制定预防或控制血吸虫病的合理方法的重要一步。我们的兴趣是研究血吸虫幼虫响应脂质而产生的蛋白水解混合物的生化和分子机制，通过该机制促进宿主细胞外基质的降解和感染性幼虫进入宿主血液。 该项目将化学蛋白质组学技术与最近可用的大量血吸虫基因组序列数据相结合，以检查促进宿主入侵的关键蛋白质成分。我们的方法包括蛋白质分离技术和蛋白质质谱分析。 然后，我们使用计算机图形实验室提供的生物信息学和序列分析工具分析衍生的蛋白质序列。 一个相关的项目是研究用于消化宿主血红蛋白和其他血浆蛋白以及传播感染的血吸虫蛋白水解酶。 具体来说，我们有兴趣绘制血吸虫消化蛋白酶的生化激活级联。 对于这些项目，我们使用 CGL 资源进行蛋白水解酶的结构建模和序列分析。