Morphology Core Facility

形态学核心设施

• 批准号: 7904821
• 负责人: JAMES E DENNIS
• 金额: $ 7.53万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7904821
• 关键词: Area; Bioreactors; Cartilage; Collaborations; Core Facility; Data; Equipment; Freezing; Frozen Sections; Gadolinium; Glycosaminoglycans; Goals; Histologic; Histology; Image; Image Analysis; Immunochemistry; Individual; Internet; Laboratories; Location; Magnetic Resonance Imaging; Measurement; Methodology; Methods; Microscope; Microscopic; Microtomy; Morphology; Plastics; Preparation; Process; Qualifying; Research; Research Project Grants; Resources; Sampling; Schedule; Services; Specimen; Specimen Handling; Staining method; Stains; Technical Expertise; Techniques; Time; Tissue Engineering; cost; experience; instrumentation; molecular imaging; programs; repaired; tissue culture

Morphology Core Facility All of the projects proposed in this Program Project rely on the use of morphological examinations and measurements as part of the analysis of their data. The goal of the Morphology Core is to provide the equipment and expertise required to process and deliver this high-quality histologic data as efficiently, and to provide expertise on the methods of sample preparation and analysis that is geared towards each particular research project. The Morphology Core is fully capable of processing specimens for a wide array of morphologic techniques including, rapid automated processing of standard histology samples, sectioning of those samples, preparation of samples tor frozen and plastic sections, immunochemistry, microscopic examination, image capture, and image analysis. The services to be provided by the Imaging Core are summarized by the following Specific Aims: Specific Aim 1: To provide a centralized processing area for the embedding, sectioning and staining of standard histologic samples. A well-equipped centralized histology laboratory with qualified technicians will prepare histologic samples using automated embedding equipment, high-end microtomy equipment for standard and frozen sections, with technical expertise, and standardized and immunohistochemical staining methodologies through the use of an experienced staff. Specific Aim 2: To provide a central laboratory for imaging and image analysis. Imaging facilities will center on a Leica upright research fluorescent and brightfield microscope and an Olympus inverted microscope for tissue culture observation plus a workstation for image analysis using Image Pro-Plus. Scheduling will be organized through the internet, as will image storage and downloading to individual laboratories. Expertise on imaging, image analysis and interpretation will be provided by the staff. Specific Aim 3: To apply non-invasive MRI for microstructral & molecular imaging of intact specimens. The Imaging Core will employ the resources of the Case Center for Imaging Research (CCIR) for routine analysis of specimens with MRI. These specialized and developing techniques include delayed Gadolinium Enhanced Magnetic Resonance Imaging of Cartilage (dGEMRIC) to assess glycosaminoglycan contents. These non-invasive methods can be applied to specimens at several time points without adversely disturbing the specimen, such as analysis of specimens in customized bioreactors. Expertise on MRI will be provided by the staff of the Imaging Core and the staff in collaboration with the staff of the CCIR. The advantages of having the Imaging Core are that the processing of samples can be conducted in one central location more cost-efficiently than in the individual labs and the centralization of the facilities allows all laboratories access to excellent equipment that can be maintained by an experienced staff. The extra advantage of this Imaging Core is the availability of experts in the field who have access to other professionals and imaging instrumentation on and off campus that may provide alternative approaches to the analysis of their specimens.

形态学核心设施 本计划项目中提出的所有项目都依赖于形态学检查的使用， 作为数据分析的一部分。Morphology Core的目标是提供 高效处理和提供高质量组织学数据所需的设备和专业知识， 提供专门知识的样品制备和分析的方法，是针对每一个特定的 研究项目。 Morphology Core完全能够处理各种形态学的标本。 这些技术包括标准组织学样品的快速自动化处理，这些样品的切片， 冰冻切片和塑料切片的样品制备，免疫化学，显微镜检查，图像 捕获和图像分析。成像核心提供的服务由 具体目标： 具体目标1：提供一个集中处理区域，用于包埋、切片和染色 标准组织学样本。 配备合格技术人员的设备齐全的中央组织学实验室将准备组织学检查。 样品使用自动包埋设备，高端切片设备的标准和冷冻 切片，具有技术专长，标准化和免疫组织化学染色方法， 使用经验丰富的工作人员。 具体目标2：为成像和图像分析提供中心实验室。 成像设备将集中在徕卡直立研究荧光和明场显微镜和 Olympus倒置显微镜用于组织培养观察，加上一个工作站用于使用Image进行图像分析 专业+。日程安排将通过互联网组织，图像存储和下载也将通过互联网组织。 个别实验室。工作人员将提供成像、图像分析和判读方面的专门知识。 具体目标3：应用非侵入性MRI对完整标本进行微结构和分子成像。 成像核心将利用成像研究案例中心（CCIR）的资源， 使用MRI对标本进行常规分析。这些专门的和发展中的技术包括延迟 钆增强磁共振成像（dGEMRIC）用于评估糖胺聚糖 内容.这些非侵入性方法可以在几个时间点应用于标本，而不会对细胞产生不利影响。 干扰样品，例如在定制的生物反应器中分析样品。MRI的专业知识将是 由成像中心的工作人员和与CCIR工作人员合作的工作人员提供。 拥有成像核心的优点是，可以在 一个中心位置比单个实验室更具成本效益，并且设施的集中化允许 所有实验室都可以使用由经验丰富的工作人员维护的优秀设备。额外的 该成像核心的优势是该领域的专家可以访问其他专业人员 以及校园内外的成像仪器，这些仪器可以为分析 他们的标本。