Morphology Core Facility

形态学核心设施

• 批准号: 8118208
• 负责人: JAMES E DENNIS
• 金额: $ 6.6万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8118208
• 关键词: Area; Bioreactors; Cartilage; Collaborations; Core Facility; Data; Equipment; Freezing; Frozen Sections; Gadolinium; Glycosaminoglycans; Goals; Histologic; Histology; Image; Image Analysis; Immunochemistry; Individual; Internet; Laboratories; Location; Magnetic Resonance Imaging; Measurement; Methodology; Methods; Microscope; Microscopic; Microtomy; Morphology; Plastics; Preparation; Process; Qualifying; Research; Research Project Grants; Resources; Sampling; Schedule; Services; Specimen; Specimen Handling; Staining method; Stains; Technical Expertise; Techniques; Time; cost; experience; instrumentation; molecular imaging; programs; tissue culture

Morphology Core Facility All of the projects proposed in this Program Project rely on the use of morphological examinations and measurements as part of the analysis of their data. The goal of the Morphology Core is to provide the equipment and expertise required to process and deliver this high-quality histologic data as efficiently, and to provide expertise on the methods of sample preparation and analysis that is geared towards each particular research project. The Morphology Core is fully capable of processing specimens for a wide array of morphologic techniques including, rapid automated processing of standard histology samples, sectioning of those samples, preparation of samples tor frozen and plastic sections, immunochemistry, microscopic examination, image capture, and image analysis. The services to be provided by the Imaging Core are summarized by the following Specific Aims: Specific Aim 1: To provide a centralized processing area for the embedding, sectioning and staining of standard histologic samples. A well-equipped centralized histology laboratory with qualified technicians will prepare histologic samples using automated embedding equipment, high-end microtomy equipment for standard and frozen sections, with technical expertise, and standardized and immunohistochemical staining methodologies through the use of an experienced staff. Specific Aim 2: To provide a central laboratory for imaging and image analysis. Imaging facilities will center on a Leica upright research fluorescent and brightfield microscope and an Olympus inverted microscope for tissue culture observation plus a workstation for image analysis using Image Pro-Plus. Scheduling will be organized through the internet, as will image storage and downloading to individual laboratories. Expertise on imaging, image analysis and interpretation will be provided by the staff. Specific Aim 3: To apply non-invasive MRI for microstructral & molecular imaging of intact specimens. The Imaging Core will employ the resources of the Case Center for Imaging Research (CCIR) for routine analysis of specimens with MRI. These specialized and developing techniques include delayed Gadolinium Enhanced Magnetic Resonance Imaging of Cartilage (dGEMRIC) to assess glycosaminoglycan contents. These non-invasive methods can be applied to specimens at several time points without adversely disturbing the specimen, such as analysis of specimens in customized bioreactors. Expertise on MRI will be provided by the staff of the Imaging Core and the staff in collaboration with the staff of the CCIR. The advantages of having the Imaging Core are that the processing of samples can be conducted in one central location more cost-efficiently than in the individual labs and the centralization of the facilities allows all laboratories access to excellent equipment that can be maintained by an experienced staff. The extra advantage of this Imaging Core is the availability of experts in the field who have access to other professionals and imaging instrumentation on and off campus that may provide alternative approaches to the analysis of their specimens.

形态核心设备 本计划项目中提出的所有项目都依赖于形态检查和 将测量作为其数据分析的一部分。形态学核心的目标是提供 需要设备和专业知识，以同样有效地处理和提供这些高质量的组织学数据，并 提供针对每个特定情况的样品制备和分析方法方面的专业知识 研究项目。 形态学核心完全有能力处理各种形态的标本 技术包括，标准组织学样本的快速自动处理，这些样本的切片， 冰冻切片和塑料切片的样品制备、免疫化学、显微镜检查和图像 捕捉和图像分析。成像核心将提供的服务由 以下是具体目标： 具体目标1：为包埋、切片和染色提供一个集中加工区 标准组织学样本。 配备齐全的集中式组织学实验室和合格的技术人员将准备组织学 样品采用自动包埋设备，高端显微切割机用于标准和冷冻 具有技术专长的切片，以及标准化和免疫组织化学染色方法 使用经验丰富的员工。 具体目标2：为成像和图像分析提供一个中心实验室。 成像设施将集中在徕卡直立式研究荧光和明场显微镜以及 用于组织培养观察的奥林巴斯倒置显微镜和用于图像分析的工作站 Pro-Plus。日程安排将通过互联网进行，图像存储和下载也将通过互联网进行 单独的实验室。工作人员将提供成像、图像分析和解释方面的专业知识。 具体目的3：将无创性磁共振应用于完整标本的微结构和分子成像。 成像核心将利用案例成像研究中心(CCIR)的资源 对标本进行常规MRI分析。这些专门的和正在开发的技术包括延迟 Gd增强软骨磁共振成像(DGEMRIC)评价糖胺多聚糖 内容。这些非侵入性方法可以在几个时间点上应用于标本，而不会产生不利影响 干扰样本，例如在定制的生物反应器中分析样本。核磁共振方面的专业知识将是 由成像核心工作人员和工作人员与CCIR工作人员合作提供。 拥有成像核心的优势在于，样品处理可以在 一个中心位置比单独的实验室更具成本效益，并且设施的集中化允许 所有实验室都可以获得优秀的设备，这些设备可以由经验丰富的员工进行维护。临时演员 该成像核心的优势是可以获得该领域的专家，他们可以联系其他专业人员 以及校内外的成像仪器，这些仪器可以为分析 他们的标本。