Rapid and cost-efficient genotyping tools for stuides of threespine stickleback

描述（申请人提供）：三刺鱼是研究数量性状发育遗传基础的新兴模式系统。社区内的研究人员一致认为，利用和增强最近完成的基因组序列的工具是必不可少的。我们建议通过向社区提供一种基于微阵列的高效和经济的基因分型系统，帮助研究人员了解棘鱼自然变化特征的潜在遗传学。我们开发的高通量基因分型系统，RAD图谱，将能够快速识别控制从行为到骨骼形状等复杂性状的基因。为了方便使用这个工具，将提供简单的网络阵列分析工具和实践课程，以培训基因组学新研究人员。棘鱼是许多实验室生物的补充模型系统，因为许多孤立和分化的自然种群已经进化出成人特征，这些特征很难或不可能通过传统的诱变方法进行研究。重要的是，许多这些性状是定量的，受遗传疾病等位基因的影响，而棘鱼是检验这些定量性状的独特脊椎动物模型。我们在这里提出的基因分型工具将极大地加速这项重要的研究。来自果蝇、斑马鱼和老鼠等其他研究群体的研究人员表示，如果这些提议的工具可用，他们将对棘鱼进行研究。因此，我们的基因组工具将通过协助目前的棘鱼研究人员，增加发现最常见人类疾病的遗传基础定量变异的速度；通过吸引更多的研究人员来研究这种新兴的模式生物。相关性：大多数常见的人类疾病，如心脏病、高血压和骨关节炎，都是个体基因与其环境复杂相互作用的结果。三刺棘鱼是这些特征的极好模型，它们有许多相似的特征，由许多基因和它们所生活的特定环境控制。我们建议创建研究工具，以使快速发现控制这些复杂性状的基因，并帮助进一步出现刺鱼作为一个重要的模型，特别是人类共同性状，以及一般的复杂性状。

DESCRIPTION (provided by applicant): Threespine stickleback fish is an emerging model system for studying the developmental genetic basis of quantitative traits. Researchers within the community agree that tools to leverage and enhance the recently completed genomic sequence are essential. We propose to help investigators understand the underlying genetics of the naturally varing traits seen in stickleback by providing to the community a microarray-based system for efficient and cost effective genotyping. The high-throughput genotyping system that we have developed, RAD mapping, will enable the rapid identification of the genes controlling complex traits ranging from behavior to bone shape. In order to facilitate the use of this tool, simple web-accessible array analysis tools and a hands-on course to train researchers new to genomics will be provided. Stickleback are a complementary model system for many laboratory-based organisms, in that the numerous isolated and divergent natural populations have evolved adult traits that are a difficult or impossible to study by traditional mutagenesis approaches. Importantly, many of these traits are quantitative, influenced by alleles at genetic diseases, and stickleback are a unique vertebrate model for examining these quantitiative traits. The genotyping tools that we propose here will greatly accelerate this important research. Investigators from other research communities such as Drosophila, zebrafish, and mouse have expressed enthusiasm for initiating work in stickleback if these proposed tools were available. Thus, our genomic tools will increase the rate of discoveries of the genetic basis quantitative variation that underlies most common human diseases both by assisting present stickleback researchers; and by drawing additional researchers to work on this emerging model organism. Relevance: Most common human diseases, such as heart disease, high blood pressure, and osteoarthritis, are the result of complex interactions between an individual's genes and her or his environment. Threespine stickleback fish are an excellent model for these kinds of traits, and have numerous traits that are similarly controlled by many genes and the particular environment in which they live. We propose to create research tools to enable the rapid discovery of the genes controlling these complex traits, and help further the emergence of stickleback as an important model for common human traits specifically, and complex traits in general.