Rapid and cost-efficient genotyping tools for stuides of threespine stickleback

描述（申请人提供）：三棘鱼是一种新兴的模式系统，用于研究数量性状的发育遗传基础。社区内的研究人员一致认为，利用和增强最近完成的基因组序列的工具至关重要。我们建议通过向社区提供基于微阵列的系统进行高效和具有成本效益的基因分型，帮助研究人员了解棘鱼中自然变化特征的潜在遗传学。我们开发的高通量基因分型系统，RAD作图，将能够快速识别控制从行为到骨骼形状的复杂性状的基因。为了促进这一工具的使用，将提供简单的网络访问阵列分析工具和实践课程，以培训新的基因组学研究人员。Stickleback是许多基于实验室的生物体的补充模型系统，因为许多孤立的和不同的自然群体已经进化出难以或不可能通过传统诱变方法研究的成年性状。重要的是，这些性状中的许多是数量的，受遗传疾病的等位基因的影响，棘鱼是一个独特的脊椎动物模型，用于检查这些数量性状。我们在这里提出的基因分型工具将大大加速这一重要研究。来自其他研究团体的研究人员，如果蝇、斑马鱼和小鼠，都表示如果这些拟议的工具可用的话，他们对开始在棘鱼中工作的热情。因此，我们的基因组工具将通过帮助现有的刺鱼研究人员和吸引更多的研究人员来研究这种新兴的模式生物，来提高发现大多数常见人类疾病的遗传基础数量变异的速度。相关性：大多数常见的人类疾病，如心脏病、高血压和骨关节炎，都是个体基因与环境之间复杂相互作用的结果。三棘鱼是这些性状的一个很好的模型，并且具有许多类似的由许多基因和它们所生活的特定环境控制的性状。我们建议创建研究工具，使控制这些复杂性状的基因的快速发现，并帮助进一步出现棘鱼作为一个重要的模型，为人类共同的特征，特别是复杂的性状一般。

DESCRIPTION (provided by applicant): Threespine stickleback fish is an emerging model system for studying the developmental genetic basis of quantitative traits. Researchers within the community agree that tools to leverage and enhance the recently completed genomic sequence are essential. We propose to help investigators understand the underlying genetics of the naturally varing traits seen in stickleback by providing to the community a microarray-based system for efficient and cost effective genotyping. The high-throughput genotyping system that we have developed, RAD mapping, will enable the rapid identification of the genes controlling complex traits ranging from behavior to bone shape. In order to facilitate the use of this tool, simple web-accessible array analysis tools and a hands-on course to train researchers new to genomics will be provided. Stickleback are a complementary model system for many laboratory-based organisms, in that the numerous isolated and divergent natural populations have evolved adult traits that are a difficult or impossible to study by traditional mutagenesis approaches. Importantly, many of these traits are quantitative, influenced by alleles at genetic diseases, and stickleback are a unique vertebrate model for examining these quantitiative traits. The genotyping tools that we propose here will greatly accelerate this important research. Investigators from other research communities such as Drosophila, zebrafish, and mouse have expressed enthusiasm for initiating work in stickleback if these proposed tools were available. Thus, our genomic tools will increase the rate of discoveries of the genetic basis quantitative variation that underlies most common human diseases both by assisting present stickleback researchers; and by drawing additional researchers to work on this emerging model organism. Relevance: Most common human diseases, such as heart disease, high blood pressure, and osteoarthritis, are the result of complex interactions between an individual's genes and her or his environment. Threespine stickleback fish are an excellent model for these kinds of traits, and have numerous traits that are similarly controlled by many genes and the particular environment in which they live. We propose to create research tools to enable the rapid discovery of the genes controlling these complex traits, and help further the emergence of stickleback as an important model for common human traits specifically, and complex traits in general.