Rapid and cost-efficient genotyping tools for stuides of threespine stickleback

描述(申请人提供)：三松刺鱼是一种研究数量性状发育遗传基础的新兴模式系统。社区内的研究人员一致认为，利用和增强最近完成的基因组序列的工具是必不可少的。我们建议通过向社区提供一个基于微阵列的系统来进行高效和成本效益的基因分型，以帮助研究人员了解刺鱼自然变异特征的潜在遗传学。我们开发的高通量基因分型系统RAD图谱将使快速识别控制从行为到骨骼形状等复杂特征的基因成为可能。为了便利这一工具的使用，将提供简单的网络可用的阵列分析工具和培训新入门的基因组学研究人员的实践课程。对于许多实验室生物来说，刺背是一个互补的模型系统，因为无数孤立和不同的自然种群进化出了成体特征，这些特征很难或不可能通过传统的诱变方法进行研究。重要的是，这些特征中的许多都是量化的，受遗传病的等位基因影响，而刺背是检验这些量化特征的独特脊椎动物模型。我们在这里提出的基因分型工具将极大地加速这项重要的研究。来自果蝇、斑马鱼和老鼠等其他研究社区的研究人员已经表示，如果这些拟议的工具可用，他们对启动刺鱼的工作充满热情。因此，我们的基因组工具将通过帮助现有的刺鱼研究人员，以及通过吸引更多的研究人员来研究这种新兴的模式生物，来提高发现最常见的人类疾病背后的遗传基础数量变异的速度。相关性：大多数常见的人类疾病，如心脏病、高血压和骨性关节炎，都是个体基因和环境之间复杂相互作用的结果。三刺鱼是这类特征的极好模型，它们拥有许多类似于许多基因和它们生活的特定环境控制的特征。我们建议创建研究工具，使控制这些复杂特征的基因能够快速发现，并有助于进一步使刺鱼成为人类共同特征的重要模型，特别是一般的复杂特征。

DESCRIPTION (provided by applicant): Threespine stickleback fish is an emerging model system for studying the developmental genetic basis of quantitative traits. Researchers within the community agree that tools to leverage and enhance the recently completed genomic sequence are essential. We propose to help investigators understand the underlying genetics of the naturally varing traits seen in stickleback by providing to the community a microarray-based system for efficient and cost effective genotyping. The high-throughput genotyping system that we have developed, RAD mapping, will enable the rapid identification of the genes controlling complex traits ranging from behavior to bone shape. In order to facilitate the use of this tool, simple web-accessible array analysis tools and a hands-on course to train researchers new to genomics will be provided. Stickleback are a complementary model system for many laboratory-based organisms, in that the numerous isolated and divergent natural populations have evolved adult traits that are a difficult or impossible to study by traditional mutagenesis approaches. Importantly, many of these traits are quantitative, influenced by alleles at genetic diseases, and stickleback are a unique vertebrate model for examining these quantitiative traits. The genotyping tools that we propose here will greatly accelerate this important research. Investigators from other research communities such as Drosophila, zebrafish, and mouse have expressed enthusiasm for initiating work in stickleback if these proposed tools were available. Thus, our genomic tools will increase the rate of discoveries of the genetic basis quantitative variation that underlies most common human diseases both by assisting present stickleback researchers; and by drawing additional researchers to work on this emerging model organism. Relevance: Most common human diseases, such as heart disease, high blood pressure, and osteoarthritis, are the result of complex interactions between an individual's genes and her or his environment. Threespine stickleback fish are an excellent model for these kinds of traits, and have numerous traits that are similarly controlled by many genes and the particular environment in which they live. We propose to create research tools to enable the rapid discovery of the genes controlling these complex traits, and help further the emergence of stickleback as an important model for common human traits specifically, and complex traits in general.