Project 2

项目2

• 批准号: 8151958
• 负责人: JAMES Jeiwen CHOU
• 金额: $ 35.31万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8151958
• 关键词: Amino Acids; Cell-Free System; Cells; Collaborations; Complex; Detergents; Development; Environment; Escherichia coli; G-Protein-Coupled Receptors; Isotope Labeling; Label; Lipids; Measurement; Membrane; Metabolic; Methods; Micelles; NMR Spectroscopy; Phospholipids; Physiologic pulse; Positioning Attribute; Process; Proteins; Protocols documentation; Resolution; Sampling; Scheme; Solutions; Structure; Technology; data acquisition; interest; microbial; mimetics; permease; reconstitution; research study; restraint

2.1. PROJECT 2 (Wagner) ¿ New Labeling Schemes, NMR Technology, and MP Structure and Function The major objectives of this project are to develop MP reconstitution and isotope labeling methods and new NMR experiments for the proposed pipeline to facilitate MP structure determination by solution NMR. These new developments, in combination with methods to be established in other projects of the Center, will enable routine NMR structure determination of MPs and characterization of MP complexes.

2.1.项目2（瓦格纳）新标记方案、NMR技术和MP结构与功能 该项目的主要目标是开发MP重构和同位素标记方法以及用于拟建管道的新NMR实验，以促进通过溶液NMR确定MP结构。这些新的发展，与该中心的其他项目中建立的方法相结合，将使常规的核磁共振结构测定MP和MP复合物的表征。