George M. O'Brien Kidney Center at Yale

项目概述（见说明）：这是耶鲁大学乔治·m·奥布莱恩肾脏中心的更新申请。该中心成立的首要目标是促进基础、转化和临床研究，以推进肾脏疾病的预防和治疗。主要研究方向为肾上皮细胞生物学和生理学；遗传性肾脏疾病与肾脏发育；急性肾损伤（AKI）和慢性肾病（CKD）；血管生物学，炎症和肾小球疾病。该中心的一个至关重要的好处是为耶鲁大学和全国各地的肾脏研究人员提供了高度专业化的服务，而不是常规的服务来支持他们的研究。为此，该中心包括三个核心，提供小动物生理学和表型服务，以便在正常动物和肾损伤和肾脏疾病动物模型的小管，肾脏和完整生物体水平上详细表征肾功能；提供小鼠遗传学和细胞系服务，开发新的动物模型和肾脏细胞系，阐明正常肾功能的分子机制和肾脏疾病的病理生理；并提供人类遗传学和临床研究服务，以提高肾脏研究人员将先进的遗传学和基因组技术应用于人类研究的能力。我们的核心目前有大约90名调查人员，其中包括40多名外部机构的用户。试点和可行性计划的目标是为年轻研究者提供初始项目资金，吸引新的研究者进入肾脏疾病研究领域，并促进与肾脏疾病直接相关的转化和临床研究。此外，通过最大化肾脏研究者和受训者之间的互动和信息共享，并通过提供活动来加强从本科生到博士后的各级受训者的招聘和教育，强化肾脏疾病研究。

PROJECT SUMMARY (See instructions): This is an application for renewal of the George M. O'Brien Kidney Center at Yale. This center was established with the overarching goal to facilitate basic, translational and clinical research that will advance the prevention and treatment of kidney diseases. Major research areas of emphasis are renal epithelial cell biology and physiology; inherited kidney disease and kidney development; acute kidney injury (AKI) and chronic kidney disease (CKD); and vascular biology, inflammation and glomerular disease. A critically important benefit of the Center is to provide renal investigators both at Yale and across the country with access to highly specialized services not otherwise routinely available to support their research. To this end, the Center includes three cores to provide small animal physiology and phenotyping services to enable detailed characterization of renal function at the level of the tubule, the kidney, and the intact organism in normal animals and in animal models of renal injury and kidney disease; provide mouse genetics and cell line services to develop novel animal models and kidney cell lines to elucidate the molecular mechanisms underlying normal renal function and the pathophysiology of kidney diseases; and provide human genetics and clinical research services to enhance the ability of renal investigators to apply advanced genetic and genomic technologies to human investigation. Our cores currently have a combined user base of approximately 90 investigators, including over 40 at outside institutions. A Pilot and Feasibility Program has the goals of providing initial project funding for young investigators, attracting new investigators into the field of kidney disease research, and fostering translational and clinical studies directly related to kidney diseases. In addition, an Enrichment Program enhances kidney disease research by maximizing interaction and information sharing among renal investigators and trainees, and by providing activities to enhance recruitment and education of all levels of trainees from undergraduate students to postdoctoral fellows.