Metabolic Research Core: energy balance and obesity studies

代谢研究核心：能量平衡和肥胖研究

• 批准号: 8149685
• 负责人: Kong Chen
• 金额: $ 39.87万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8149685
• 关键词: Metabolic; Obesity; Research; energy balance

We discovered a systemic problem with our whole-room indirect calorimetry suites (commissioned in August 2008). During the winter of this year, we detected significant artifacts in all three chambers that lead to unstable performances. After rigorous testing, we traced the issue to extreme low relative humidity of the building supply air as it introduced high differential partial pressure (of water vapor) between the chamber and reference air. We engaged with building engineers and officials from Office of Facility Management, Office of Research Facilities, and the Clinical Center. We confirmed our findings of low humidity and are currently working towards a three-prong approach to rectify the issue: (1) tighter humidity control of the Metabolic Unit supply air to provide a more stable environment by the OFM, (2) use the Clinical Center medical air as supply air for the calorimetry suites, and (3) revamp the calorimetry air sample and condition equipment, calibration gas mixing, and analyzer upgrades. With the strong support from NIDDK leadership and Clinical Center, we are making steady progress in these critical renovations. Research findings: In 25 young and normal-weight healthy volunteers, Dr. Celi and his colleagues have found that a 12-hr environmental temperature change (from 24 to 19 C) produced a 6% increase in resting energy expenditure as measured by our metabolic suites. This increase was likely a result of the activation of brown adipose tissue via the sympathetic nervous system, but less of an influence by the thyroid hormone. While expanded studies are underway to study obese and elderly individuals and to further quantify the brown adipose tissue activity by FDG-PET imaging, we have published the current findings. We are continuing our intra/extramural collaboration project with Dr. Naji Abumrad from Vanderbilt University to study the changes in energy metabolism, protein turnover, insulin action, and associated weight loss before and after Roux-en-Y gastric bypass surgery. The Vanderbilt study has stopped new recruitment but we following up the patients that we studied on whole-body metabolism during rapid weight loss. We have published a manuscript in Obesity recently. Services rendered (FY09 activities): 24-hr energy expenditure by whole-room respiratory chambers (299), resting energy expenditure by metabolic carts (364), exercise and physical function testing (144), body composition (581 Bod Pod measurements, 388 iDXA scans), objective physical activity monitoring (588), food-array experimental dinning (146), muscle biopsies (28), subcutaneous fat biopsies (79), and Health and Physical Exams (75). All activities are significantly up from FY08, despite of the humidity issue with the metabolic chambers.

我们在整个房间间接的量热法套件中发现了一个系统性问题（2008年8月委托）。在今年的冬季，我们在所有三个腔室中都发现了大量的伪影，这导致表演不稳定。经过严格的测试后，我们将问题追溯到建筑物的极低相对湿度，因为它在腔室和参考空气之间引入了高差分压（水蒸气）。我们与设施管理办公室，研究设施办公室和临床中心的建筑工程师和官员互动。我们确认了低湿度的发现，目前正在努力纠正该问题：（1）对代谢单位的湿度更严格供应空气，以提供更稳定的环境，（（2）使用临床中心医疗空气作为量热仪的供应空气，用于量热计套件，以及（3）对升温的空气量和调节设备进行量化量和分析量和分析量和分析量和分析量和分析。在NIDDK领导力和临床中心的大力支持下，我们在这些关键的翻新中取得了稳步的进步。 研究结果：在25名年轻和正常的健康志愿者中，Celi博士及其同事发现，通过我们的代谢套件来衡量的静息能量消耗增加了12小时的环境温度变化（从24到19 C）增加了6％。这种增加可能是由于交感神经系统激活棕色脂肪组织的结果，但甲状腺激素的影响较小。尽管正在进行扩大的研究研究肥胖和老年人，并通过FDG-PET成像进一步量化了棕色脂肪组织活性，但我们已经发表了当前的发现。 我们正在与范德比尔特大学的Naji Abumrad博士继续我们的壁外协作项目，以研究能量代谢，蛋白质更新，胰岛素作用，胰岛素作用以及相关的胃胃旁路旁路手术前后相关的体重减轻的变化。范德比尔特（Vanderbilt）的研究已经停止了新的招聘，但是我们跟进了快速减肥期间研究全身代谢的患者。我们最近发表了肥胖症的手稿。 渲染的服务（09财年）：全室呼吸器室的24小时能量消耗（299），代谢推车的静息能量支出（364）（364），运动和身体功能测试（144），身体组成，身体组成，581 BOD POD测量值（388 IDXA SCANS，388 IDXA SCANS，388 IDXA SCANS），客观的体育活动监控（588），DIT（588），DIT（588），食品，妈妈，妈妈，588 （28），皮下脂肪活检（79），以及健康与身体检查（75）。尽管代谢室存在湿度问题，但所有活动都比08财年大大提高。