CONFORMATIONAL DYNAMICS IN ION CHANNEL SELECTIVITY AND GATING

离子通道选择性和门控中的构象动力学

基本信息

项目摘要

 DESCRIPTION (provided by applicant): Ion channels control both the resting membrane potential and the action potential in cells. As a result, improper physiological function of ion channels leads to a wide variety of disease states, including cardiac and neurological dysfunction, epilepsy, asthma, hearing loss, and others. Proper ion channel function requires appropriate ion selectivity and channel gating to control ion flux across biological membranes. However, our understanding of these fundamental properties of ion channels is still incomplete because they arise from a complex interplay of structure and dynamics. We will use solution NMR to experimentally test the role of protein dynamics in ion selectivity and gating of the NaK channel from Bacillus cereus solubilized in isotropic bicelles. NaK is an ideal model system because small mutations can shift its selectivity; its structure is similar to eukaryotic potassium channel pore domains, particularly biomedically important cyclic nucleotide gated and HERG potassium channels; and it is highly stable and amenable to structural and biophysical characterization. NMR provides a unique method to simultaneously and quantitatively probe ion channel structure and dynamics with site-specific resolution. The ability of NMR to measure dynamics on diverse timescales and detect transient and lowly populated states allows us to experimentally evaluate the role of protein dynamics on processes ranging from ion selectivity to channel gating. In this proposal we will use NMR studies of NaK to test three aims: Does selectivity arise from cooperative effects of ion binding on backbone dynamics? How does the N-terminal M0 helix regulate NaK gating? What structural and dynamic features allow allosteric communication between the selectivity filter and inner gate in potassium channels?


项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Katherine Anne Henzler-Wildman其他文献

Katherine Anne Henzler-Wildman的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Katherine Anne Henzler-Wildman', 18)}}的其他基金

Expanding the Scope of NMR Sample Preparation
扩大 NMR 样品制备的范围
  • 批准号:
    10089600
  • 财政年份:
    2021
  • 资助金额:
    $ 29.72万
  • 项目类别:
Expanding the Scope of NMR Sample Preparation
扩大 NMR 样品制备的范围
  • 批准号:
    10573324
  • 财政年份:
    2021
  • 资助金额:
    $ 29.72万
  • 项目类别:
NMR Technologies for Integrating Structure, Function and Disease
整合结构、功能和疾病的 NMR 技术
  • 批准号:
    10089598
  • 财政年份:
    2021
  • 资助金额:
    $ 29.72万
  • 项目类别:
Molecular Mechanisms of Channels and Transporters
通道和转运蛋白的分子机制
  • 批准号:
    10608951
  • 财政年份:
    2021
  • 资助金额:
    $ 29.72万
  • 项目类别:
NMR Technologies for Integrating Structure, Function and Disease
整合结构、功能和疾病的 NMR 技术
  • 批准号:
    10323282
  • 财政年份:
    2021
  • 资助金额:
    $ 29.72万
  • 项目类别:
Molecular Mechanisms of Channels and Transporters
通道和转运蛋白的分子机制
  • 批准号:
    10394922
  • 财政年份:
    2021
  • 资助金额:
    $ 29.72万
  • 项目类别:
Expanding the Scope of NMR Sample Preparation
扩大 NMR 样品制备的范围
  • 批准号:
    10323284
  • 财政年份:
    2021
  • 资助金额:
    $ 29.72万
  • 项目类别:
Molecular Mechanisms of Channels and Transporters
通道和转运蛋白的分子机制
  • 批准号:
    10204502
  • 财政年份:
    2021
  • 资助金额:
    $ 29.72万
  • 项目类别:
NMR Technologies for Integrating Structure, Function and Disease
整合结构、功能和疾病的 NMR 技术
  • 批准号:
    10573321
  • 财政年份:
    2021
  • 资助金额:
    $ 29.72万
  • 项目类别:
CONFORMATIONAL DYNAMICS IN ION CHANNEL SELECTIVITY AND GATING
离子通道选择性和门控中的构象动力学
  • 批准号:
    9133430
  • 财政年份:
    2015
  • 资助金额:
    $ 29.72万
  • 项目类别:

相似海外基金

Positive Affect and Pediatric Asthma: An Innovative Positive Psychology Model to Improve Asthma Management and Health
积极情绪与小儿哮喘:改善哮喘管理和健康的创新积极心理学模型
  • 批准号:
    10712703
  • 财政年份:
    2023
  • 资助金额:
    $ 29.72万
  • 项目类别:
How the immune response can affect influenza virus and asthma
免疫反应如何影响流感病毒和哮喘
  • 批准号:
    nhmrc : GNT1054081
  • 财政年份:
    2013
  • 资助金额:
    $ 29.72万
  • 项目类别:
    Early Career Fellowships
How do thick airway walls affect airway hyperresponsiveness in asthma?
气道壁厚如何影响哮喘气道高反应性?
  • 批准号:
    nhmrc : 512387
  • 财政年份:
    2008
  • 资助金额:
    $ 29.72万
  • 项目类别:
    NHMRC Project Grants
WHAT FAMILY PROCESSES AFFECT CHILDHOOD ASTHMA OUTCOME?
哪些家庭过程会影响儿童哮喘的结局?
  • 批准号:
    6566335
  • 财政年份:
    2000
  • 资助金额:
    $ 29.72万
  • 项目类别:
WHAT FAMILY PROCESSES AFFECT CHILDHOOD ASTHMA OUTCOME?
哪些家庭过程会影响儿童哮喘的结局?
  • 批准号:
    6504483
  • 财政年份:
    2000
  • 资助金额:
    $ 29.72万
  • 项目类别:
WHAT FAMILY PROCESSES AFFECT CHILDHOOD ASTHMA OUTCOME?
哪些家庭过程会影响儿童哮喘的结局?
  • 批准号:
    6304309
  • 财政年份:
    1999
  • 资助金额:
    $ 29.72万
  • 项目类别:
WHAT FAMILY PROCESSES AFFECT CHILDHOOD ASTHMA OUTCOME?
哪些家庭过程会影响儿童哮喘的结局?
  • 批准号:
    6114202
  • 财政年份:
    1998
  • 资助金额:
    $ 29.72万
  • 项目类别:
The way in which newly discovered protein messengers in the lung affect asthma
肺部新发现的蛋白质信使影响哮喘的方式
  • 批准号:
    nhmrc : 970947
  • 财政年份:
    1997
  • 资助金额:
    $ 29.72万
  • 项目类别:
    NHMRC Project Grants
WHAT FAMILY PROCESSES AFFECT CHILDHOOD ASTHMA OUTCOME?
哪些家庭过程会影响儿童哮喘的结局?
  • 批准号:
    6275437
  • 财政年份:
    1997
  • 资助金额:
    $ 29.72万
  • 项目类别:
WHAT FAMILY PROCESSES AFFECT CHILDHOOD ASTHMA OUTCOME
哪些家庭过程会影响儿童哮喘的结局
  • 批准号:
    6245226
  • 财政年份:
    1997
  • 资助金额:
    $ 29.72万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了