Developing New Inbred Zebrafish Lines to Enhance Cell Transplantation Models
开发新的近交斑马鱼系以增强细胞移植模型
基本信息
- 批准号:9010991
- 负责人:
- 金额:$ 18.8万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-02-10 至 2018-01-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAgingAnimal ModelAnimal TestingAnimalsAutologousBone MarrowCaenorhabditis elegansCardiovascular DiseasesCell TherapyCell TransplantationCell TransplantsCellsCommunitiesComplexCongenital AbnormalityDevelopmentDevelopmental BiologyDiseaseDrosophila genusFishesGenerationsGenesGoalsHealthHumanImmuneImmunosuppressionInbreedingIndividualInjuryLengthLibrariesLongevityMalignant NeoplasmsMental DepressionMethodsModelingMonitorMusOpportunistic InfectionsOrganOrthologous GenePartner in relationshipPhysiologyProblem SolvingReportingSkinTestingTimeTissuesTransplantationTransplanted tissueZebrafishcostdesigngene environment interactiongene functiongraft vs host diseasehuman diseasemeetingsmutantnervous system disordernovelnovel strategiesnovel therapeuticspreclinical studyrepairedresearch studysexsomatic cell nuclear transferstem cell biologysuccess
项目摘要
DESCRIPTION (provided by applicant): The long-term goal of this proposal is to facilitate the implementation of novel human cell therapies. The success of such treatments much depends on having reliable animal models to perform proof of principle experiments and preclinical studies. Zebrafish have been tested in transplantation experiments such as bone marrow and skin, however, the lack of immune-compatible fish seriously hinders its broad use. We propose to develop a new approach to generate transplantation-compatible zebrafish lines. This new method should be capable of deploying rapidly, at low cost; potentially capitalizing on the extensive and rapidly-expanding library of mutants modeling human disease. Our specific aims are: Aim 1, to develop MHC compatible zebrafish for three strains of fish used by the biomedical community i.e. Tubingen, AB and Casper lines. We will accomplish this aim using somatic cell nuclear transfer. We will produce clones of both sexes from an individual fish that will be subsequently crossed; Aim 2, to demonstrate, using functional studies, the capacity of these animals to tolerate transplanted cells from matched donors without the use of immunosuppression; we will test animals of each generation for their immune compatibility using reciprocal skin transplants. We are addressing a critical barrier in the field by solving the
problem of reliably generating immune- compatible zebrafish. In addition, the same strategy can be used for the generation of homozygous zebrafish lines in a considerably shorter period of time. New and already developed mutant zebrafish lines could be re-derived on a homozygous background enhancing their value as model organism.
描述(由申请人提供):本提案的长期目标是促进新型人类细胞疗法的实施。此类治疗的成功在很大程度上取决于是否有可靠的动物模型来进行原理验证实验和临床前研究。斑马鱼已经在骨髓和皮肤等移植实验中进行了测试,然而,缺乏免疫相容性鱼类严重阻碍了其广泛应用。我们建议开发一种新的方法来产生移植兼容的斑马鱼线。这种新方法应该能够以低成本快速部署;潜在地利用广泛和快速扩展的突变体库来模拟人类疾病。我们的具体目标是:目的1,为生物医学界使用的三种鱼类(即Tubingen系、AB系和Casper系)开发MHC相容性斑马鱼。我们将通过体细胞核移植来实现这一目标。我们将从随后杂交的个体鱼中产生两性克隆;目的2,使用功能研究来证明这些动物耐受来自匹配供体的移植细胞而不使用免疫抑制的能力;我们将使用相互皮肤移植来测试每一代动物的免疫相容性。我们正在解决该领域的一个关键障碍,
可靠地产生免疫相容的斑马鱼的问题。此外,相同的策略可以用于在相当短的时间内产生纯合斑马鱼系。新的和已经开发的突变斑马鱼品系可以在纯合背景上重新衍生,提高它们作为模式生物的价值。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A 13-plex of tetra- and penta-STRs to identify zebrafish.
用于识别斑马鱼的 13 重四重和五重 STR。
- DOI:10.1038/s41598-020-60842-5
- 发表时间:2020
- 期刊:
- 影响因子:4.6
- 作者:Venta,PatrickJ;Nguyen,AnthonyK;Senut,Marie-Claude;Poulos,WilliamG;Prukudom,Sukumal;Cibelli,JoseB
- 通讯作者:Cibelli,JoseB
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JOSE B CIBELLI其他文献
JOSE B CIBELLI的其他文献
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{{ truncateString('JOSE B CIBELLI', 18)}}的其他基金
Establishing an Integrated Platform for Diploid Germplasm Conservation in Zebrafish (Danio rerio)
建立斑马鱼二倍体种质保护综合平台
- 批准号:
10556907 - 财政年份:2023
- 资助金额:
$ 18.8万 - 项目类别:
Establishing an Integrated Platform for Diploid Germplasm Conservation in Zebrafish (Danio rerio) – A&R
建立斑马鱼(Danio rerio)二倍体种质保护综合平台 – A
- 批准号:
10808534 - 财政年份:2023
- 资助金额:
$ 18.8万 - 项目类别:
Cryopreservation and Cloning of Somatic Cells to Preserve Zebrafish Germplasm
体细胞的冷冻保存和克隆以保存斑马鱼种质
- 批准号:
9048319 - 财政年份:2016
- 资助金额:
$ 18.8万 - 项目类别:
CREATION OF CLONED CATTLE LACKING THE PRION GENE
缺乏朊病毒基因的克隆牛的创造
- 批准号:
2792696 - 财政年份:1999
- 资助金额:
$ 18.8万 - 项目类别:
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