Distribution, Provenance, and Human Health Implications of Marine Polybrominated

海洋多溴化合物的分布、来源和人类健康影响

• 批准号: 8708085
• 负责人: Lihini Aluwihare
• 金额: $ 10.71万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8708085
• 关键词: Age; Aquaculture; Area; Biological; Biota; Body Burden; California; Cattle; Collaborations; Consumption; Cyanobacterium; Data; Development; Diet; Dietary intake; Dolphins; Domestic Fowls; Embryo; Environment; Epidemiology; Exposure to; Farming environment; Fisheries; Fishes; Food Webs; Gas Chromatography; Habitats; Habits; Hake; Head; Health; Human; Human Milk; Human body; Invertebrates; Investigation; Isotopes; Link; Liver; Mammals; Marines; Marketing; Mass Spectrum Analysis; Measurement; Meat; Metabolic Biotransformation; Methods; Modeling; Oceans; Online Systems; Organism; Participant; Perch; Phase; Populations at Risk; Questionnaires; Recording of previous events; Red Algae; Research; Research Design; Resources; Risk; Role; Route; Sample Size; Sampling; Scombridae; Seafood; Source; Squid; Stomach Content; Study Section; Testing; Time; Toxic effect; Tuna; Work; Zebrafish; analog; analytical method; anthropogenesis; base; coastal water; design; dibenzo(1,4)dioxin; exposed human population; food consumption; interest; novel; pentabromodiphenyl ether; phenyl ether; pollutant; sex; stable isotope

Recently the distribution of polybrominated diphenyl ethers (PBDE analogs) - the methoxylated and hydroxylated derivatives (MeO-BDE and OH-BDE) and polybrominated dibenzo-p-dioxins (PBDDs)- as well as congeners of the halogenated methyl bipyrroles (MBP) and dimethyl bipyrroles (DMBP) have been examined in a variety of wildlife and humans, where they can often be present at higher concentrations than anthropogenic pollutants. Like anthropogenic pollutants they also bioaccumulate, seem to biomagnify, and toxicity has been demonstrated for at least a subset! However, systematic studies aimed at linking the distribution of HOCs present in apex marine predators to potential source environments through examination of prey and primary producers (a focus of PI and P2) are rare. In this proposal, Aluwihare and Hoh build on an existing collaboration to combine a non-targeted analytical method with a food web-based research approach to assess sources and fates of the entire suite of HOCs in the Southern California Bight Region. In the initial phase of this project the relatively high concentration and "unlimited" sample size represented by stranded marine mammals will be leveraged to identify and definitively characterize the suite of HOCs (> 200) bioaccumulating in wildlife. This non-targeted approach is facilitated by a novel application of comprehensive wo-dimensional gas chromatography with time- of-flight mass spectrometry (GCxGC/TOF-MS), and relies on collaboration with NOAA and the Southern California Coastal Water Resources Project (SCCWRP). Discovery of new compounds identified by the non-targeted method, will be aided by a variety of authentic standards provided for us by PI and P2. To definitively identify HOC contributions from marine biogenic sources a compound specific radiocarbon and stable isotope approach will also be applied. A recently designed 2D preparative GC will make this research possible. To further establish source, collaborations with PI and P2 will screen a variety of habitat specific primary producers, lower trophic level organisms and pure bacterial cultures for their ability to make HOCs of interest. These major areas of research are tightly integrated with the Analytical Core. To assess human exposure, relevant HOCs will be examined in breast milk samples, and epidemiological investigations will assist in the interpretation of these results with respect to participants' seafood consumption habits. This work will be carried out in collaboration with Christina Chambers, Michelle Leff and Jae Kim (all at UCSD), These data will further inform a study design to examine HOC distributions in relevant seafood products available to consumers. The exposure studies are planned to ultimately assess HOC dietary intake by age and sex from food consumption, and will be done in collaboration with Melbourne Hovell and Jenny Quintana (SDSU). A final component, headed by Kristin Pangallo (Colgate) will begin to interrogate the toxicity of a subset of natural HOCs synthesized and/or isolated by PI and P2. Delineating sources and fates of marine natural HOCs with respect to human health will better inform fisheries management and aquaculture practices, assist with quantifying risks associated with diet, and help to design relevant multiple exposure studies with respect to HOC toxicity.

最近，多溴联苯醚(PBDE类似物)-甲氧基化和羟化衍生物(MeO-BDE和OH-BDE)和多溴联苯并对二恶英(PBDDs)-以及卤化甲基联吡咯(MBP)和二甲基联吡咯(DMBP)的同系物在各种野生动物和人类中的分布被研究，它们的存在浓度往往高于人为污染物。就像人为污染物一样，它们也会生物积累，似乎会生物放大， 而且至少有一个子集的毒性已经被证明！然而，旨在通过检查猎物和初级生产者(PI和P2的重点)将顶端海洋捕食者体内存在的HOCs的分布与潜在来源环境联系起来的系统研究很少。在这项提案中，Aluwihare和Hoh建立在现有合作的基础上，将非定向分析方法与基于食物网络的研究方法相结合，以评估南加州海湾整套HOC的来源和命运 地区。在该项目的初始阶段，将利用相对较高的浓度和以搁浅的海洋哺乳动物为代表的“无限”样本大小来识别和确定在野生动物中生物积累的HOCs(&gt；200)套件。这种非靶向的方法是通过一种新的应用-飞行时间质谱仪的综合二维气相色谱来促进的 (GCxGC/TOF-MS)，并依赖于与NOAA和南加州沿海水资源项目(SCCWRP)的合作。通过非靶向方法鉴定的新化合物的发现，将得到PI和P2为我们提供的各种真实标准的帮助。为了最终确定海洋生物来源的特殊贡献，还将采用化合物、特定放射性碳和稳定同位素方法。最近设计的2D制备型GC将使这一研究成为可能。为了进一步确定源头，与PI和P2的合作将筛选各种特定于生境的初级生产者， 低营养水平的生物和纯细菌培养，因为它们有能力产生感兴趣的HOC。这些主要研究领域与分析核心紧密结合。为了评估人类接触情况，将在母乳样本中检查相关的HOC，流行病学调查将有助于解释这些结果与参与者的海鲜消费习惯有关。这项工作将与克里斯蒂娜·钱伯斯、米歇尔·莱夫和Jae Kim(都在加州大学圣地亚哥分校)合作进行，这些数据将进一步为研究设计提供信息，以检查相关海鲜产品的特殊分布。 消费者。暴露研究计划最终评估饮食消费中年龄和性别的特殊饮食摄入量，并将与墨尔本霍维尔和珍妮·昆塔纳(SDSU)合作完成。由Kristin Pangallo(高露洁)领导的最后一个组件将开始询问由PI和P2合成和/或分离的天然HOCs的子集的毒性。从人类健康的角度描述海洋天然HOCs的来源和命运将更好地为渔业管理和水产养殖实践提供信息，帮助量化与饮食有关的风险，并有助于设计与特定毒性有关的相关多重暴露研究。