Facility Core: Small Molecule Mass Spectrometry Facility for Analytical & Prep.

设施核心：小分子质谱分析设施

• 批准号: 8413093
• 负责人: Lihini Aluwihare
• 金额: $ 3.22万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8413093
• 关键词: Advisory Committees; Analytical Chemistry; Appointment; Budgets; Businesses; California; Collection; Communities; Consumption; Core Facility; Detection; Electrons; Environment; Fermentation; Funding; Gases; Goals; Habitats; Hand; Health; High Pressure Liquid Chromatography; Housing; Human; Human Resources; Individual; Institution; Investigation; Ions; Isotopes; Liquid Chromatography; Maintenance; Marines; Mass Fragmentography; Mass Spectrum Analysis; Measurement; Minor; Mission; Monitor; Oceanography; Oceans; Organism; Pathway interactions; Performance; Population; Postdoctoral Fellow; Preparation; Prevalence; Production; Recording of previous events; Research; Research Infrastructure; Research Personnel; Research Support; Resolution; Resources; Role; Sampling; Schedule; Screening procedure; Seafood; Services; Source; Students; Temperature; Time; Tissue Extracts; Training; Vaporizer; Visit; base; detector; experience; graduate student; instrument; instrumentation; interdisciplinary collaboration; interest; liquid chromatography mass spectrometry; meetings; member; multidisciplinary; operation; small molecule; stable isotope; success; theories

Facility Core Summary The Facility Core for Small Molecule Mass Spectrometry (SMMS) is integral to the Center's research mission by enabling high-resolution detection and isolation of halogenated organic compounds (HQCs). This Core does not replicate any analytical services or facilities currently available at the Scripps Institution of Oceanography (SIO). The SMMS Facility Core will be directed by P3 Leader Aluwihare, and technical oversight for the facility will be provided Co-Director Dr. Yongxuan Su. Daily operation and routine maintenance will be the responsibility of Mr. Matthew Woolery. The facility will include several uniquely configured gas chromatography-mass spectrometry (GC-MS) instruments that are intimately integrated with the Center's research mission to identify the suite of HOCs accumulating in the marine environment, discover new HOCs of concern in wildlife and humans, and evaluate environmental sources of HOCs. The overall goal of PI is to identify organisms and habitats of interest with respect to HOC production in the Southern California Bight. A GC Electron Capture Detector (|JECD)/MS capable of screening these samples for relevant HOC production will be available through the Core. Project 2 aims to identify and characterize the prevalence of polybrominated HOC biosynthetic pathways in the marine environment. As such, P2 will rely on the same instrumentation to screen cultures and insure that organisms under investigation are producing compounds of interest. The overall goalof P3 is to identify the suite of small, natural HOCs available to enter human populations through seafood consumption. This research will be supported by the analytical capabilities of two GCXGC instruments integrated into the proposed Facility. The >200 HOCs that bioaccumulate in apex predators and humans cannot be adequately separated from co-eluting compounds for definitive identification and quantification without comprehensive GCXGC separation. To establish the biogenic origin of HOCs of interest, P3 will also conduct stable isotope studies on pure compounds that are isolated from apex predators, purified and concentrated for isotope measurement. A uniquely configured, preparative GCXGC instrument that is included in the Facility will support this research. Two liquid chromatography-mass spectrometry (LC-MS) instruments will also be housed in the Core to analyze fermentation extracts and synthetic organic preparations. The Internal Advisory Committee will discuss Facility business during monthly meetings, and the External Advisory Committee will evaluate the Facility Core for its performance annually.

贷款核心摘要 小分子质谱（SMMS）设施核心是该中心的研究使命的组成部分，使高分辨率检测和卤代有机化合物（HQC）的分离。 该核心不复制斯克里普斯海洋研究所（SIO）目前提供的任何分析服务或设施。SMMS设施核心将由P3负责人Jumwihare指导，设施的技术监督将由联合主任Su Yongxuan博士提供。日常操作和日常维护将由Matthew Woolery先生负责。该设施将包括几个独特配置的气相色谱-质谱（GC-MS）仪器， 该中心的研究使命是确定海洋环境中积累的一套HOCs，发现野生动物和人类中新的HOCs，并评估HOCs的环境来源。PI的总体目标是确定南加州湾HOC生产相关的生物和栖息地。气相色谱电子捕获检测器（|能够筛选这些样本以进行相关HOC生产的JECD/MS将通过核心获得。项目2旨在确定和 描述海洋环境中多溴HOC生物合成途径的普遍性。因此，P2将依赖于相同的仪器来筛选培养物，并确保所研究的生物体正在产生感兴趣的化合物。P3的总体目标是确定一套小的，天然的HOCs可通过海鲜消费进入人群。这项研究将由两个GCXGC仪器的分析能力集成到拟议的设施的支持。 在没有全面的GCXGC分离的情况下，无法从共洗脱化合物中充分分离出在顶端捕食者和人类中生物累积的>200种HOCs，以进行明确的鉴定和定量。为了确定感兴趣的HOCs的生物来源，P3还将对从顶端捕食者中分离、纯化和浓缩以进行同位素测量的纯化合物进行稳定同位素研究。一个独特的配置，准备GCXGC仪器，包括在该设施将支持这项研究。两个液相色谱-质谱（LC-MS）仪器也将被安置在核心，以分析发酵提取物和合成有机制剂。内部咨询委员会将在月度会议上讨论贷款业务，外部咨询委员会将每年评估贷款核心的绩效。