Exploring synaptic remodeling with graphene optoelectronic probes

用石墨烯光电探针探索突触重塑

基本信息

  • 批准号:
    9025171
  • 负责人:
  • 金额:
    $ 23.01万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-04-01 至 2018-03-31
  • 项目状态:
    已结题

项目摘要

 DESCRIPTION (provided by applicant): The activity and plasticity of dendritic spines and synapses underlie normal cognitive processes, such as learning and memory and are the basis for the complex circuitry found in the brain. Dendritic spines, which are actin-rich protrusions that emanate from the dendrite shaft, comprise most postsynaptic terminals of excitatory synapses. Not surprisingly, abnormalities in dendritic spines are associated with a number of neurological disorders, including Fragile-X syndrome, Down's syndrome, Alzheimer's disease, autism, schizophrenia, and epilepsy. Despite the importance of spines and synapses in the central nervous system, the molecular mechanisms that regulate the activity and plasticity of these structures are not well understood largely because of the current lack of available technologies for probing these structures at single spine/synapse levels. Furthermore, the capability to study synaptic activity and plasticity in individual spines and synapses would provide significant insight into the function and molecular mechanisms that regulate these structures. We are developing novel neuron-glia co-culture microfluidic devices with integrated graphene sensors and electrodes and combining them with scanning photocurrent microscopy to detect and stimulate spine plasticity at sub- synaptic resolution (Specific Aim I). We will use this technology to record electrical properties at individual dendritic spines and synapses and to examine the effects of different electrical stimuli on these structures. Since reorganization of te actin cytoskeleton is thought to underlie the activity, plasticity, and function of dendritic spine and synapses, we will explore the role of actin-binding protein VASP in regulating synaptic activity and plasticity (Specific Aim II). We will alter the expression of VASP and determine the effect on the electrical properties of individual dendritic spines and synapses with the graphene probes. Moreover, we will determine the contribution of this protein to synaptic plasticity. The development of the proposed microfluidic platforms will be of great interest and benefit to neurobiologists by providing a powerful technology for investigating the mechanisms that underlie the electrical activity and plasticity of dendritic spines and synapses at a single synaps level.


项目成果

期刊论文数量(0)
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Deyu Li其他文献

Deyu Li的其他文献

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{{ truncateString('Deyu Li', 18)}}的其他基金

Mutational spectra of bulky DNA lesions
大体积 DNA 损伤的突变谱
  • 批准号:
    10312790
  • 财政年份:
    2018
  • 资助金额:
    $ 23.01万
  • 项目类别:
Mapping Brain Activity with High Spatiotemporal Resolution using Graphene Probes
使用石墨烯探针以高时空分辨率绘制大脑活动图
  • 批准号:
    10244939
  • 财政年份:
    2017
  • 资助金额:
    $ 23.01万
  • 项目类别:
Inhibitory Effect of Nitric Oxide on DNA Repair Enzymes
一氧化氮对DNA修复酶的抑制作用
  • 批准号:
    9232253
  • 财政年份:
    2017
  • 资助金额:
    $ 23.01万
  • 项目类别:
Exploring synaptic remodeling with graphene optoelectronic probes
用石墨烯光电探针探索突触重塑
  • 批准号:
    9234603
  • 财政年份:
    2016
  • 资助金额:
    $ 23.01万
  • 项目类别:
Imaging synapse formation using novel microfluidic platforms
使用新型微流体平台对突触形成进行成像
  • 批准号:
    8094187
  • 财政年份:
    2011
  • 资助金额:
    $ 23.01万
  • 项目类别:
Imaging synapse formation using novel microfluidic platforms
使用新型微流体平台对突触形成进行成像
  • 批准号:
    8306755
  • 财政年份:
    2011
  • 资助金额:
    $ 23.01万
  • 项目类别:

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