photo-initiated entrapping of chromatographic particles for high resolution microfluidic and capillary based seperation

用于高分辨率微流体和毛细管分离的色谱颗粒的光引发捕获

• 批准号: 345347-2007
• 负责人: Oleschuk, Richard
• 金额: $ 10.4万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=325959
• 关键词: photo; initiated; entrapping; chromatographic; particles

Proteomic and genomic studies have generated an enormous amount of samples for various types of analysis. Current analytical techniques are not able to keep up with the current rate of demand creating an analysis bottleneck. Microfluidics, the manipulation of extremely small volumes of fluids is seen as a possible solution to mounting analysis backlog. The handling of liquids on such a small scale has a number of advantages for the field of analytical chemistry including the ability to qualitatively and quantitatively analyze samples as small as a few picoliters at greatly enhanced speeds. Also as a result of miniaturization most processes involved with the analysis can be integrated within a single microchip. This proposal outlines research into the development of a novel light induced fabrication process to immobilize commercial reagent laden microspheres in capillaries and lab-on-a-chip devices. The microspheres, with various functionalized surfaces, will be used to perform chemical separations, reactions and enhance detection. The novel microsphere/polymer materials described within this proposal will enable microspheres to be photo-patterned anywhere within a device greatly simplifying construction and will ultimately increase the types of analyses conducted within a chip. The goal is to integrate the hybrid materials into low cost polymeric devices to develop a powerful, yet inexpensive proteomic tool.

蛋白质组学和基因组学研究已经产生了大量的样品用于各种类型的分析。当前的分析技术无法跟上当前的需求速度，从而造成分析瓶颈。微流体技术，即对极少量流体的操作，被视为解决分析积压问题的一种可能方案。在这种小规模上处理液体对于分析化学领域具有许多优点，包括以大大提高的速度定性和定量分析小至几皮升的样品的能力。此外，由于微型化，大多数与分析有关的过程可以集成在单个微芯片内。该提案概述了一种新型光诱导制造工艺的开发研究，以在毛细管和芯片实验室设备中将商业试剂负载微球包裹起来。具有各种功能化表面的微球将用于进行化学分离、反应和增强检测。本提案中描述的新型微球/聚合物材料将使微球能够在设备内的任何地方进行光图案化，从而大大简化了结构，并最终增加了在芯片内进行的分析类型。我们的目标是将混合材料整合到低成本的聚合物装置中，以开发一种功能强大但价格低廉的蛋白质组学工具。