photo-initiated entrapping of chromatographic particles for high resolution microfluidic and capillary based seperation

蛋白质组学和基因组学研究为各种类型的分析产生了大量的样本。目前的分析技术无法跟上当前的需求速度，造成了分析瓶颈。在微流控技术中，对极少量流体的处理被视为处理大量分析积压的一种可能解决方案。如此小规模的液体处理对分析化学领域有许多优势，包括能够以极大的提高速度对小至几皮升的样品进行定性和定量分析。此外，由于小型化的结果，与分析有关的大多数过程都可以集成在单个微芯片中。这项建议概述了开发一种新的光诱导制造工艺的研究，以将商业试剂负载的微球固定在毛细血管和芯片实验室设备中。具有各种功能化表面的微球将被用于进行化学分离、反应和增强检测。这项提案中描述的新型微球/聚合物材料将使微球能够在设备内的任何位置进行光图案化，极大地简化了结构，并最终将增加在芯片内进行的分析类型。其目标是将杂化材料集成到低成本的聚合设备中，以开发一种强大而廉价的蛋白质组学工具。

Proteomic and genomic studies have generated an enormous amount of samples for various types of analysis. Current analytical techniques are not able to keep up with the current rate of demand creating an analysis bottleneck. Microfluidics, the manipulation of extremely small volumes of fluids is seen as a possible solution to mounting analysis backlog. The handling of liquids on such a small scale has a number of advantages for the field of analytical chemistry including the ability to qualitatively and quantitatively analyze samples as small as a few picoliters at greatly enhanced speeds. Also as a result of miniaturization most processes involved with the analysis can be integrated within a single microchip. This proposal outlines research into the development of a novel light induced fabrication process to immobilize commercial reagent laden microspheres in capillaries and lab-on-a-chip devices. The microspheres, with various functionalized surfaces, will be used to perform chemical separations, reactions and enhance detection. The novel microsphere/polymer materials described within this proposal will enable microspheres to be photo-patterned anywhere within a device greatly simplifying construction and will ultimately increase the types of analyses conducted within a chip. The goal is to integrate the hybrid materials into low cost polymeric devices to develop a powerful, yet inexpensive proteomic tool.