Tissue culture facility expansion

几十年来，组织培养扩大了生物系统的研究。它为研究人员提供了一个解决基本问题的高度易处理的细胞生物学工具。最近几年，干细胞的研究几乎完全基于从生物体中提取的细胞在组织培养中生长的能力。在某些情况下，它消除了在研究中使用动物的需要。组织培养现在被研究人员用来研究一系列生物体，包括植物、昆虫和哺乳动物。我们的部门包括研究各种动物的研究人员，从果蝇(果蝇)到老鼠。我们中的几个人正在研究哺乳动物(Tropepe，Varmuza)和昆虫(Tepass，Godt，Ranguette，Romans)的组织培养系统，我们中的一个正在开发斑马鱼组织培养系统(Tropepe)。现有的组织培养设施最初是为一个实验室(Tropepe)使用而设计的，但随着更多研究人员(Varmuza、Godt、Tepass、Ranguette)的使用，已经经历了巨大的压力。此外，现有设施最近已被重新归类为生物安全遏制级别2(BL2)，以促进构建慢病毒载体，供两个实验室(Tropepe，Varmuza)用于哺乳动物细胞和胚胎。我们现在需要组装额外的组织培养能力，以供使用非哺乳动物细胞(Godt，Tepass，Ranguette，Tropepe，Romans)或BL1遏制水平的哺乳动物细胞(Tropepe，Varmuza)的其他研究人员使用。为此目的，该部门已提供空间安装生物安全柜、两个保温箱、一个冰箱、一个冰柜和一个液氮储存容器。进行高级细胞研究的能力将使我们能够为知识库和高素质人员(HQP)的培训做出重大贡献。

Tissue culture has augmented research in biological systems for several decades. It has provided investigators with a highly tractable cell biological tool for addressing basic questions. In more recent years, the study of stem cells has been based almost entirely on the ability of cells extracted from organisms to grow in tissue culture. In some cases, it has obviated the need to use animals in research. Tissue culture is now used by investigators working with a range of organisms, including plants, insects and mammals. Our department includes investigators working with a variety of animals, from Drosophila melanogaster (fruit flies) to mice. Several of us are working with mammalian (Tropepe, Varmuza) and insect (Tepass, Godt, Ringuette, Romans) tissue culture systems, and one of us is developing a zebrafish tissue culture system (Tropepe). The existing tissue culture facility, originally designed for use by one lab (Tropepe), has experienced tremendous pressure by the advent of use by additional investigators (Varmuza, Godt, Tepass, Ringuette). In addition, the existing facility has recently been reclassified to Biological Safety Containment Level 2 (BL2)  in order to facilitate the construction of lentivirus vectors for use in mammalian cells and embryos by two labs (Tropepe, Varmuza). We now need to assemble additional tissue culture capacity for use by other investigators using either non-mammalian cells (Godt, Tepass, Ringuette, Tropepe, Romans) or mammalian cells at BL1 containment levels (Tropepe, Varmuza). The department has provided space for the installation of a biosafety cabinet, two incubators, a refrigerator, a freezer and a liquid nitrogen storage container for this purpose. The ability to conduct advanced cell research will allow us to make significant contributions to the knowledge base, and to training of highly qualified personnel (HQP).