Atomic absorption spectrophotometer for acid-base and ion transport studies in fish

需要经费购买原子吸收分光光度计系统，用于分析阳离子（Na+、Ca2+、Mg2+和K+）。AAS是分析阳离子的首选方法，因为它是唯一一种足够灵敏的方法，可用于测量稀释样品（如淡水）中的阳离子，以及测量动物与其环境之间离子交换引起的离子浓度的微小变化。 此外，它是可靠的，适合于高通量，并具有相对低的运行成本。 研究淡水鱼体内的离子迁移和酸碱平衡，完全依赖于原子吸收光谱法对离子浓度的测定。 我们现有的AAS被大量使用，但已经有13年的历史了，不再得到制造商的支持，而且很快就失败了。 当它不再可操作时，渥太华大学生物系内几个人的研究计划将被严重削减。 因此，我们请求资金购买替代原子吸收分光光度计系统，以支持我们对鱼类离子转运和酸碱调节机制的研究。

Funds are requested for the purchase of an atomic absorption spectrophotometry (AAS) system for the analysis of cations (Na+, Ca2+, Mg2+ and K+). AAS is the method of choice for the analysis of cations because it is the only method that is sufficiently sensitive for the measurement of cations in dilute samples (such as freshwater), and for the measurement of small changes in ion concentrations resulting from ion exchange between the animal and its environment.  Moreover, it is reliable, amenable to high throughput, and has relatively low running costs.  Studies of ion transport and acid-base balance in freshwater fish rely absolutely on the measurement of ion concentrations by AAS.  Our existing AAS is heavily used, but is 13 years old, no longer supported by its manufacturer, and failing rapidly.  When it is no longer operable, the research programs of several individuals within the Department of Biology at the University of Ottawa will be severely curtailed.  Thus, we request funds for the purchase of a replacement atomic absorption spectrophotometry system to support our research into the mechanisms of ion transport and acid-base regulation in fish.