Atomic absorption spectrophotometer for acid-base and ion transport studies in fish

用于鱼类酸碱和离子传输研究的原子吸收分光光度计

• 批准号: 345254-2007
• 负责人: Gilmour, Kathleen
• 金额: $ 3.26万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326200
• 关键词: Atomic; absorption; spectrophotometer; acid; base

Funds are requested for the purchase of an atomic absorption spectrophotometry (AAS) system for the analysis of cations (Na+, Ca2+, Mg2+ and K+). AAS is the method of choice for the analysis of cations because it is the only method that is sufficiently sensitive for the measurement of cations in dilute samples (such as freshwater), and for the measurement of small changes in ion concentrations resulting from ion exchange between the animal and its environment.  Moreover, it is reliable, amenable to high throughput, and has relatively low running costs.  Studies of ion transport and acid-base balance in freshwater fish rely absolutely on the measurement of ion concentrations by AAS.  Our existing AAS is heavily used, but is 13 years old, no longer supported by its manufacturer, and failing rapidly.  When it is no longer operable, the research programs of several individuals within the Department of Biology at the University of Ottawa will be severely curtailed.  Thus, we request funds for the purchase of a replacement atomic absorption spectrophotometry system to support our research into the mechanisms of ion transport and acid-base regulation in fish.

需要资金购买用于分析阳离子（Na+、Ca2+、Mg2+ 和 K+）的原子吸收分光光度法 (AAS) 系统。 AAS 是分析阳离子的首选方法，因为它是唯一能够足够灵敏地测量稀释样品（例如淡水）中的阳离子以及测量动物与其环境之间的离子交换引起的离子浓度微小变化的方法。  此外，它是可靠的，适合高吞吐量，并且具有相对较低的运行成本。  淡水鱼中离子传输和酸碱平衡的研究完全依赖于原子吸收光谱法对离子浓度的测量。  我们现有的 AAS 被大量使用，但已有 13 年历史，制造商不再支持，并且很快就会失败。  当它不再运作时，渥太华大学生物系内几个人的研究项目将受到严重限制。  因此，我们请求资金购买替代原子吸收分光光度测定系统，以支持我们对鱼类离子传输和酸碱调节机制的研究。