Microplate luminometer

微孔板发光计

• 批准号: 359163-2008
• 负责人: Rivoal, Jean
• 金额: $ 2.26万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=362198
• 关键词: Microplate; luminometer

Funds are requested for the purchase of a microplate luminometer. This apparatus will be used to support research in plant metabolism, biological rhythms and signal transduction. The apparatus measures light emitted from biological samples.  It is the instrument of choice to measure ATP, an indicator of the energetic status of the cell.  It will also be used to measure luciferase, a marker widely used in bioassays and in the study of biological rhythms. The instrument is able to process up to 96 samples simultaneously. This feature reduces the time needed for analysis. The various applications for the instrument require a temperature control of the sample and 2 injectors to deliver reagents.   The requested equipment is not currently available in our Institute.  The development of the research programs of the 3 applicants depends heavily on the accessibility to a microplate luminometer.  This instrument will be heavily used and will become an additional resource to all the students and research personnel of our Institute.

请求提供经费购买一台微板光度计。该仪器将用于支持植物代谢、生物节律和信号转导的研究。该仪器测量生物样品发出的光，是测量ATP（细胞能量状态的指示器）的首选仪器，也将用于测量荧光素酶（生物测定和生物节律研究中广泛使用的标记物）。该仪器能够同时处理多达96个样品。此功能减少了分析所需的时间。该仪器的各种应用需要样品温度控制和2个注射器来输送试剂。 我们研究所目前没有所需的设备。三位申请人的研究计划的发展在很大程度上取决于对微板光度计的可及性。该仪器将被大量使用，并将成为我们研究所所有学生和研究人员的额外资源。