Multimode plate reader to support research in plant biochemistry, cell biology and ecology

请拨资金购买多模式车牌阅读器。所要求的仪器将用于执行各种实验，以支持植物生物化学、细胞生物学和生态学的研究计划。它专为高通量处理、分析和定量微量样品中的生物产品而设计。该仪器是多功能的，可利用吸光度、荧光和发光以微孔板格式分析样品。它还配备了一个细胞仪，可以进行基础显微镜和微孔板格式的细胞成像。所要求的仪器配置还包括盒式附件，其通过允许执行时间分辨荧光分析来扩展仪器的灵活性和分析能力，例如用于研究蛋白质之间相互作用的荧光共振能量转移、生物发光共振能量转移和荧光偏振检测。所要求的仪器配置还能够使用Eu标记的二次抗体对膜上的少量蛋白质进行定量。这一特征在复杂提取物中蛋白质的定量或蛋白质修饰的研究中是有用的。由于其多模式设计，所要求的平板读取器将取代两个不再起作用且过于昂贵而无法维修的平板读取器。它还将取代我们单位因最近一名研究人员离开另一所大学而丢失的一些显微镜设备。它将为我们研究所带来开发新研究项目所需的新的分析能力。所要求的设备将整合到我们研究所的蛋白质组学和生物化学平台中，该平台的功能是用户付费的。它将由一名合格的研究助理负责，负责新用户的培训和仪器的定期维护。多模平板阅读器将被所有四个共同申请者的实验室成员大量使用，因为他们的研究计划的许多方面依赖于功能强大的平板阅读器的可用性。它还将为我们研究所在分子和细胞生物学以及植物化学领域工作的学生和研究人员提供额外的资源。其他学术或行业研究人员也将根据平台的费用结构访问设备。受过这种仪器培训的高素质人员将获得宝贵的实践专业知识，拥有最先进的仪器，可用于就业市场上的各种基础和应用研究项目。

Funds are requested for the purchase of a multi-mode plate reader. The requested instrument will be used to perform a variety of experiments to support research programs in plant biochemistry, cell biology and ecology. It is designed for high-throughput processing, analysis and quantification of bio-products in micro-volumes of samples. This instrument is multifunctional and designed to analyze samples in microplate format using absorbance, fluorescence and luminescence. It is also equipped with a cytometer that allows to perform basic microscopy and cellular imaging in microplate format. The requested configuration of the instrument also includes cartridges add-ons that expend the flexibility and analytical capabilities of the instrument by allowing to perform time-resolved fluorescence assays for applications like Fluorescence Resonance Energy Transfer, Bioluminescence Resonance Energy Transfer and Fluorescence Polarization detection used for example in the study of interactions between proteins. The requested instrument configuration is also capable of quantifying small amounts of proteins on membranes using Europium-labeled secondary antibodies. This feature is useful in the quantification of proteins in complex extracts or in the study of protein modifications. Because of its multimode design, the requested plate reader will replace two plate readers that are no longer functional and too costly to repair. It will also replace some of the microscopy equipment lost by our unit due to the recent departure of a researcher to another university. It will bring to our Institute new analytical capabilities needed for the development of new research projects. The requested equipment will be integrated in the Proteomics and Biochemistry Platform of our Institute which functions on a user-fee basis. It will be under the responsibility of a qualified research assistant who will be in charge of the training of new users and the regular maintenance of the instrument. The multimode plate reader will be heavily used by the laboratory members of all four co-applicants because many aspects of their research programs depend on the availability of a functioning plate reader. It will also serve as an additional resource for the students and research personnel of other groups in our Institute who work in the areas of molecular and cellular biology and in phytochemistry. Other academic or industry researchers will also have access to the equipment according to the fee structure of the platform. Highly Qualified Personnel trained with this instrument will gain valuable hands-on expertise with a state of the art apparatus that can be used in a variety of fundamental and applied research projects on the job market.